LOCUS       BBa_I3300               1844 bp    DNA     linear       22-Dec-2024
DEFINITION  Tar-EnvZ attractant receiver device (R1075.B0034.C0082.B0015.R0082)
ACCESSION   BBa_I3300
VERSION     BBa_I3300.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    498..499
                     /label=A
     misc_feature    1528..1560
                     /label=LVA
     CDS             76..840
                     /label=Tar (front)
     CDS             847..1527
                     /label=Envz (back)
     misc_feature    1567..1591
                     /label=Help:Barcodes
     CDS             76..1566
                     /label=BBa_C0082
     stem_loop       1611..1654
                     /label=stem_loop
     misc_feature    1600..1679
                     /label=BBa_B0010
     terminator      1600..1679
                     /label=BBa_B0010
     stem_loop       1695..1714
                     /label=T7 TE
     misc_feature    1721..1721
                     /label=stop
     polyA_site      1715..1728
                     /label=polya
     misc_feature    1688..1728
                     /label=BBa_B0012
     terminator      1688..1728
                     /label=BBa_B0012
     misc_feature    1749..1766
                     /label=C1 OmpR
     misc_feature    1770..1787
                     /label=C2 OmpR
     misc_feature    1790..1807
                     /label=C3 OmpR
     promoter        1811..1816
                     /label=-35
     promoter        1834..1839
                     /label=-10
     promoter        1737..1844
                     /label=BBa_R0082
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatgat taaccgtatc cgcgtagtca cgctgttggt aatggtgctg
      121 ggggtattcg cactgttaca gcttatttcc ggcagtctgt ttttttcttc ccttcaccat
      181 agccagaaga gctttgtggt ttccaatcaa ttacgggaac agcagggcga gctgacgtca
      241 acctgggatt taatgctgca aacgcgcatt aacctgagtc gttcagcggt acggatgatg
      301 atggattcct ccaatcaaca aagtaacgcc aaagttgaat tgctcgatag cgccaggaaa
      361 acattggcgc aggcagcgac gcattataaa aaattcaaaa gcatggcacc gttacctgaa
      421 atggtcgcta ccagtcgtaa tattgatgaa aaatataaaa actattacac agcgttaact
      481 gaactgattg attatctcga ttatggcaat actggagctt atttcgctca gccaacccag
      541 ggaatgcaaa atgcaatggg cgaagcgttt gctcagtacg ccctcagcag tgaaaaactg
      601 tatcgcgata tcgtcactga caacgcagat gattaccgat ttgcccagtg gcaactggcg
      661 gttatcgcgc tggtggtggt attgattctg ctggtggcgt ggtacggcat tcgccgtatg
      721 ttgcttactc cgctggcaaa aattattgct cacattcgcg aaatcgccgg tggtaacctg
      781 gcgaataccc tgaccattga cgggcgcagt gaaatgggcg acctggcgca gagcgtttca
      841 catatggcgg ctggtgttaa gcaactggcg gatgaccgca cgctgctgat ggcgggggta
      901 agtcacgact tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat gatgagcgag
      961 caggatggct atctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt
     1021 gagcagttta tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctt
     1081 aatgcagtac tcggtgaggt gattgctgcc gaaagtggct atgagcggga aattgaaacc
     1141 gcgctttacc ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa acgcgcggtg
     1201 gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct ggatcaaagt cagcagcgga
     1261 acggagccga atcgcgcctg gttccaggtg gaagatgacg gtccgggaat tgcgccggaa
     1321 caacgtaagc acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc
     1381 acgggattag ggctggcaat tgtgcagcgt atcgtggata accataacgg gatgctggag
     1441 cttggcacca gcgagcgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg
     1501 cgggcgcagg gcacgacaaa agaaggggct gcaaacgacg aaaactacgc tttagtagct
     1561 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     1621 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1681 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc
     1741 ttgcatttac attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat
     1801 catattcgtg ttggattatt ctgcattttt ggggagaatg gact
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