BBa_I3301
type: ComponentDefinition
persistent identity: BBa I3301
display id: BBa_I3301
version: 1
was generated by: iGEM to SBOL conversion
description: Tar-EnvZ attractant receiver device (R1075.B0034.C0082.B0015.R0083)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: Constitutive promoter makes Tar-EnvZ fusion protein, which binds aspartate to create TIPS.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_C0082
component: BBa_B0034
component: BBa_R1075
component: BBa_R0083
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_R1075
sequence annotation: BBa_B0034
sequence annotation: BBa_C0082
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0083
sequence: https://synbiohub.org/public/igem/BBa_I3301_sequence/1

BBa_C0082
type: Component
persistent identity: component955988
display id: component955988
version: 1
top level: BBa_I3301
definition: tar-envZ
access: public

BBa_B0034
type: Component
persistent identity: component955972
display id: component955972
version: 1
top level: BBa_I3301
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component955967
display id: component955967
version: 1
top level: BBa_I3301
definition: const.
access: public

BBa_R0083
type: Component
persistent identity: component956024
display id: component956024
version: 1
top level: BBa_I3301
definition: OmpR
access: public

BBa_B0012
type: Component
persistent identity: component956004
display id: component956004
version: 1
top level: BBa_I3301
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component955994
display id: component955994
version: 1
top level: BBa_I3301
definition: BBa_B0010
access: public

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation955967
display id: annotation955967
version: 1
top level: BBa_I3301
location: https://synbiohub.org/public/igem/BBa_I3301/annotation955967/range955967/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I3301/annotation955967/range955967/1
type: Range
persistent identity: range955967
display id: range955967
version: 1
top level: BBa_I3301
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation955972
display id: annotation955972
version: 1
top level: BBa_I3301
location: https://synbiohub.org/public/igem/BBa_I3301/annotation955972/range955972/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3301/annotation955972/range955972/1
type: Range
persistent identity: range955972
display id: range955972
version: 1
top level: BBa_I3301
direction: direction/forward
start: 58
end: 69
orientation: inline

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation955988
display id: annotation955988
version: 1
top level: BBa_I3301
location: https://synbiohub.org/public/igem/BBa_I3301/annotation955988/range955988/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_I3301/annotation955988/range955988/1
type: Range
persistent identity: range955988
display id: range955988
version: 1
top level: BBa_I3301
direction: direction/forward
start: 76
end: 1566
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation955994
display id: annotation955994
version: 1
top level: BBa_I3301
location: https://synbiohub.org/public/igem/BBa_I3301/annotation955994/range955994/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3301/annotation955994/range955994/1
type: Range
persistent identity: range955994
display id: range955994
version: 1
top level: BBa_I3301
direction: direction/forward
start: 1600
end: 1679
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation956004
display id: annotation956004
version: 1
top level: BBa_I3301
location: https://synbiohub.org/public/igem/BBa_I3301/annotation956004/range956004/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3301/annotation956004/range956004/1
type: Range
persistent identity: range956004
display id: range956004
version: 1
top level: BBa_I3301
direction: direction/forward
start: 1688
end: 1728
orientation: inline

BBa_R0083
type: SequenceAnnotation
persistent identity: annotation956024
display id: annotation956024
version: 1
top level: BBa_I3301
location: https://synbiohub.org/public/igem/BBa_I3301/annotation956024/range956024/1
component: BBa_R0083

https://synbiohub.org/public/igem/BBa_I3301/annotation956024/range956024/1
type: Range
persistent identity: range956024
display id: range956024
version: 1
top level: BBa_I3301
direction: direction/forward
start: 1737
end: 1814
orientation: inline

tar-envZ
type: ComponentDefinition
persistent identity: BBa C0082
display id: BBa_C0082
version: 1
was generated by: iGEM to SBOL conversion
description: Receptor, tar-envZ
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding sequence for Tar-EnvZ fusion protein. Tar is the bacterial chemotaxis receptor for aspartate. EnvZ is an osmosensor kinase, which, through phosphorylation of OmpR, regulates transcription of porin genes. This fusion protein combines the extracellular and transmembrane domains of Tar with the intracellular domain of EnvZ. The fusion converts chemical binding of aspartate to production of transcription factors.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A silent point mutation was made at base 423 (A -> C) to eliminate an XbaI restriction enzyme site. The mutation was designed to match codons with similar frequencies of usage in E. coli.
star: false
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Tar (front)
sequence annotation: A
sequence annotation: Envz (back)
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0082_sequence/1

Tar (front)
type: SequenceAnnotation
persistent identity: annotation308352
display id: annotation308352
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1
type: Range
persistent identity: range308352
display id: range308352
version: 1
top level: tar-envZ
direction: direction/forward
start: 1
end: 765
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation300943
display id: annotation300943
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1
type: Range
persistent identity: range300943
display id: range300943
version: 1
top level: tar-envZ
direction: direction/forward
start: 423
end: 424
orientation: inline

Envz (back)
type: SequenceAnnotation
persistent identity: annotation308360
display id: annotation308360
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1
type: Range
persistent identity: range308360
display id: range308360
version: 1
top level: tar-envZ
direction: direction/forward
start: 772
end: 1452
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation301146
display id: annotation301146
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation301146/range301146/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0082/annotation301146/range301146/1
type: Range
persistent identity: range301146
display id: range301146
version: 1
top level: tar-envZ
direction: direction/forward
start: 1453
end: 1485
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213997
display id: annotation2213997
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation2213997/range2213997/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0082/annotation2213997/range2213997/1
type: Range
persistent identity: range2213997
display id: range2213997
version: 1
top level: tar-envZ
direction: direction/forward
start: 1492
end: 1516
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Promoter II
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
top level: const.
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
top level: const.
direction: direction/forward
start: 35
end: 40
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0083
display id: BBa_R0083
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown. The a at base 41 is mutated to t to avoid XbaI site. Speculation that this was a previous assembly site.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: C1 OmpR
sequence annotation: linker
sequence annotation: A
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0083_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301221
display id: annotation301221
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0083/annotation301221/range301221/1
type: Range
persistent identity: range301221
display id: range301221
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

linker
type: SequenceAnnotation
persistent identity: annotation301223
display id: annotation301223
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_R0083/annotation301223/range301223/1
type: Range
persistent identity: range301223
display id: range301223
version: 1
top level: OmpR
direction: direction/forward
start: 37
end: 44
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation306881
display id: annotation306881
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_R0083/annotation306881/range306881/1
type: Range
persistent identity: range306881
display id: range306881
version: 1
top level: OmpR
direction: direction/forward
start: 41
end: 41
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301224
display id: annotation301224
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0083/annotation301224/range301224/1
type: Range
persistent identity: range301224
display id: range301224
version: 1
top level: OmpR
direction: direction/forward
start: 45
end: 50
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301226
display id: annotation301226
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0083/annotation301226/range301226/1
type: Range
persistent identity: range301226
display id: range301226
version: 1
top level: OmpR
direction: direction/forward
start: 68
end: 73
orientation: inline

https://synbiohub.org/public/igem/BBa_I3301_sequence/1
type: Sequence
persistent identity: BBa I3301 sequence
display id: BBa_I3301_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgattaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtctgtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacgtcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatcaacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaattcaaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcgttaactgaactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatgcaatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatgattaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcattcgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccctgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0082_sequence/1
type: Sequence
persistent identity: BBa C0082 sequence
display id: BBa_C0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtctgtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacgtcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatcaacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaattcaaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcgttaactgaactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatgcaatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatgattaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcattcgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccctgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1075_sequence/1
type: Sequence
persistent identity: BBa R1075 sequence
display id: BBa_R1075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0083_sequence/1
type: Sequence
persistent identity: BBa R0083 sequence
display id: BBa_R0083_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgatctcttgagttggattattctgcatttttggggagaatggact
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z