BBa_I3310
type: ComponentDefinition
persistent identity: BBa I3310
display id: BBa_I3310
version: 1
was generated by: iGEM to SBOL conversion
description: Test: Tar-EnvZ attractant receiver (I3300.E0422)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: Tests Tar-EnvZ attractant receiver device (ompC upstream version) by attaching TIPS output to CFP reporter.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000804
role: partType/Project
component: BBa_B0012
component: BBa_B0010
component: BBa_C0082
component: BBa_B0034
component: BBa_R1075
component: BBa_E0022
component: BBa_R0082
sequence annotation: BBa_R1075
sequence annotation: BBa_B0034
sequence annotation: BBa_C0082
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0082
sequence annotation: BBa_E0022
sequence: https://synbiohub.org/public/igem/BBa_I3310_sequence/1

BBa_B0012
type: Component
persistent identity: component968285
display id: component968285
version: 1
top level: BBa_I3310
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component968275
display id: component968275
version: 1
top level: BBa_I3310
definition: BBa_B0010
access: public

BBa_C0082
type: Component
persistent identity: component968269
display id: component968269
version: 1
top level: BBa_I3310
definition: tar-envZ
access: public

BBa_B0034
type: Component
persistent identity: component968253
display id: component968253
version: 1
top level: BBa_I3310
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component968248
display id: component968248
version: 1
top level: BBa_I3310
definition: const.
access: public

BBa_B0012
type: Component
persistent identity: component968340
display id: component968340
version: 1
top level: BBa_I3310
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component968330
display id: component968330
version: 1
top level: BBa_I3310
definition: BBa_B0010
access: public

BBa_E0022
type: Component
persistent identity: component968322
display id: component968322
version: 1
top level: BBa_I3310
definition: ECFP
access: public

BBa_B0034
type: Component
persistent identity: component968310
display id: component968310
version: 1
top level: BBa_I3310
definition: BBa_B0034
access: public

BBa_R0082
type: Component
persistent identity: component968305
display id: component968305
version: 1
top level: BBa_I3310
definition: OmpR
access: public

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation968248
display id: annotation968248
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968248/range968248/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I3310/annotation968248/range968248/1
type: Range
persistent identity: range968248
display id: range968248
version: 1
top level: BBa_I3310
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation968253
display id: annotation968253
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968253/range968253/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3310/annotation968253/range968253/1
type: Range
persistent identity: range968253
display id: range968253
version: 1
top level: BBa_I3310
direction: direction/forward
start: 58
end: 69
orientation: inline

BBa_C0082
type: SequenceAnnotation
persistent identity: annotation968269
display id: annotation968269
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968269/range968269/1
component: BBa_C0082

https://synbiohub.org/public/igem/BBa_I3310/annotation968269/range968269/1
type: Range
persistent identity: range968269
display id: range968269
version: 1
top level: BBa_I3310
direction: direction/forward
start: 76
end: 1566
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation968275
display id: annotation968275
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968275/range968275/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3310/annotation968275/range968275/1
type: Range
persistent identity: range968275
display id: range968275
version: 1
top level: BBa_I3310
direction: direction/forward
start: 1600
end: 1679
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation968285
display id: annotation968285
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968285/range968285/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3310/annotation968285/range968285/1
type: Range
persistent identity: range968285
display id: range968285
version: 1
top level: BBa_I3310
direction: direction/forward
start: 1688
end: 1728
orientation: inline

BBa_R0082
type: SequenceAnnotation
persistent identity: annotation968305
display id: annotation968305
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968305/range968305/1
component: BBa_R0082

https://synbiohub.org/public/igem/BBa_I3310/annotation968305/range968305/1
type: Range
persistent identity: range968305
display id: range968305
version: 1
top level: BBa_I3310
direction: direction/forward
start: 1737
end: 1844
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation968310
display id: annotation968310
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968310/range968310/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3310/annotation968310/range968310/1
type: Range
persistent identity: range968310
display id: range968310
version: 1
top level: BBa_I3310
direction: direction/forward
start: 1853
end: 1864
orientation: inline

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation968322
display id: annotation968322
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968322/range968322/1
component: BBa_E0022

https://synbiohub.org/public/igem/BBa_I3310/annotation968322/range968322/1
type: Range
persistent identity: range968322
display id: range968322
version: 1
top level: BBa_I3310
direction: direction/forward
start: 1871
end: 2632
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation968330
display id: annotation968330
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968330/range968330/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3310/annotation968330/range968330/1
type: Range
persistent identity: range968330
display id: range968330
version: 1
top level: BBa_I3310
direction: direction/forward
start: 2641
end: 2720
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation968340
display id: annotation968340
version: 1
top level: BBa_I3310
location: https://synbiohub.org/public/igem/BBa_I3310/annotation968340/range968340/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3310/annotation968340/range968340/1
type: Range
persistent identity: range968340
display id: range968340
version: 1
top level: BBa_I3310
direction: direction/forward
start: 2729
end: 2769
orientation: inline

tar-envZ
type: ComponentDefinition
persistent identity: BBa C0082
display id: BBa_C0082
version: 1
was generated by: iGEM to SBOL conversion
description: Receptor, tar-envZ
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: NC_000913 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding sequence for Tar-EnvZ fusion protein. Tar is the bacterial chemotaxis receptor for aspartate. EnvZ is an osmosensor kinase, which, through phosphorylation of OmpR, regulates transcription of porin genes. This fusion protein combines the extracellular and transmembrane domains of Tar with the intracellular domain of EnvZ. The fusion converts chemical binding of aspartate to production of transcription factors.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: A silent point mutation was made at base 423 (A -> C) to eliminate an XbaI restriction enzyme site. The mutation was designed to match codons with similar frequencies of usage in E. coli.
star: false
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Tar (front)
sequence annotation: A
sequence annotation: Envz (back)
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0082_sequence/1

Tar (front)
type: SequenceAnnotation
persistent identity: annotation308352
display id: annotation308352
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0082/annotation308352/range308352/1
type: Range
persistent identity: range308352
display id: range308352
version: 1
top level: tar-envZ
direction: direction/forward
start: 1
end: 765
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation300943
display id: annotation300943
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0082/annotation300943/range300943/1
type: Range
persistent identity: range300943
display id: range300943
version: 1
top level: tar-envZ
direction: direction/forward
start: 423
end: 424
orientation: inline

Envz (back)
type: SequenceAnnotation
persistent identity: annotation308360
display id: annotation308360
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0082/annotation308360/range308360/1
type: Range
persistent identity: range308360
display id: range308360
version: 1
top level: tar-envZ
direction: direction/forward
start: 772
end: 1452
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation301146
display id: annotation301146
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation301146/range301146/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0082/annotation301146/range301146/1
type: Range
persistent identity: range301146
display id: range301146
version: 1
top level: tar-envZ
direction: direction/forward
start: 1453
end: 1485
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213997
display id: annotation2213997
version: 1
top level: tar-envZ
location: https://synbiohub.org/public/igem/BBa_C0082/annotation2213997/range2213997/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0082/annotation2213997/range2213997/1
type: Range
persistent identity: range2213997
display id: range2213997
version: 1
top level: tar-envZ
direction: direction/forward
start: 1492
end: 1516
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Promoter II
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
top level: const.
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
top level: const.
direction: direction/forward
start: 35
end: 40
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

ECFP
type: ComponentDefinition
persistent identity: BBa E0022
display id: BBa_E0022
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced cyan fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0021</bb_part>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Cyan fluorescent protein (ECFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0022 cyan fluorescent protein is based on BioBrick part BBa_E0021. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: BBa_E0022
sequence annotation: CFP (LVA)
sequence annotation: A
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0022_sequence/1

BBa_E0022
type: SequenceAnnotation
persistent identity: annotation7040
display id: annotation7040
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0022/annotation7040/range7040/1
type: Range
persistent identity: range7040
display id: range7040
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

CFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2150
display id: annotation2150
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0022/annotation2150/range2150/1
type: Range
persistent identity: range2150
display id: range2150
version: 1
top level: ECFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation2155
display id: annotation2155
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_E0022/annotation2155/range2155/1
type: Range
persistent identity: range2155
display id: range2155
version: 1
top level: ECFP
direction: direction/forward
start: 69
end: 69
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2153
display id: annotation2153
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_E0022/annotation2153/range2153/1
type: Range
persistent identity: range2153
display id: range2153
version: 1
top level: ECFP
direction: direction/forward
start: 719
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2154
display id: annotation2154
version: 1
top level: ECFP
location: https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_E0022/annotation2154/range2154/1
type: Range
persistent identity: range2154
display id: range2154
version: 1
top level: ECFP
direction: direction/forward
start: 757
end: 762
orientation: inline

https://synbiohub.org/public/igem/BBa_I3310_sequence/1
type: Sequence
persistent identity: BBa I3310 sequence
display id: BBa_I3310_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccaccatactagagaaagaggagaaatactagatgattaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtctgtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacgtcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatcaacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaattcaaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcgttaactgaactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatgcaatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatgattaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcattcgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccctgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0082_sequence/1
type: Sequence
persistent identity: BBa C0082 sequence
display id: BBa_C0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgattaaccgtatccgcgtagtcacgctgttggtaatggtgctgggggtattcgcactgttacagcttatttccggcagtctgtttttttcttcccttcaccatagccagaagagctttgtggtttccaatcaattacgggaacagcagggcgagctgacgtcaacctgggatttaatgctgcaaacgcgcattaacctgagtcgttcagcggtacggatgatgatggattcctccaatcaacaaagtaacgccaaagttgaattgctcgatagcgccaggaaaacattggcgcaggcagcgacgcattataaaaaattcaaaagcatggcaccgttacctgaaatggtcgctaccagtcgtaatattgatgaaaaatataaaaactattacacagcgttaactgaactgattgattatctcgattatggcaatactggagcttatttcgctcagccaacccagggaatgcaaaatgcaatgggcgaagcgtttgctcagtacgccctcagcagtgaaaaactgtatcgcgatatcgtcactgacaacgcagatgattaccgatttgcccagtggcaactggcggttatcgcgctggtggtggtattgattctgctggtggcgtggtacggcattcgccgtatgttgcttactccgctggcaaaaattattgctcacattcgcgaaatcgccggtggtaacctggcgaataccctgaccattgacgggcgcagtgaaatgggcgacctggcgcagagcgtttcacatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctggatcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaagatgacggtccgggaattgcgccggaacaacgtaagcacctgttccagccgtttgtccgcggcgacagtgcgcgcaccattagcggcacgggattagggctggcaattgtgcagcgtatcgtggataaccataacgggatgctggagcttggcaccagcgagcggggcgggctttccattcgcgcctggctgccagtgccggtaacgcgggcgcagggcacgacaaaagaaggggctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1075_sequence/1
type: Sequence
persistent identity: BBa R1075 sequence
display id: BBa_R1075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0022_sequence/1
type: Sequence
persistent identity: BBa E0022 sequence
display id: BBa_E0022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctggggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacatcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z