LOCUS       BBa_I3311               2739 bp    DNA     linear       22-Nov-2024
DEFINITION  Test: Tar-EnvZ attractant receiver (I3301.E0422)
ACCESSION   BBa_I3311
VERSION     BBa_I3311.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    498..499
                     /label=A
     misc_feature    1528..1560
                     /label=LVA
     CDS             76..840
                     /label=Tar (front)
     CDS             847..1527
                     /label=Envz (back)
     misc_feature    1567..1591
                     /label=Help:Barcodes
     CDS             76..1566
                     /label=BBa_C0082
     stem_loop       1611..1654
                     /label=stem_loop
     misc_feature    1600..1679
                     /label=BBa_B0010
     terminator      1600..1679
                     /label=BBa_B0010
     stem_loop       1695..1714
                     /label=T7 TE
     misc_feature    1721..1721
                     /label=stop
     polyA_site      1715..1728
                     /label=polya
     misc_feature    1688..1728
                     /label=BBa_B0012
     terminator      1688..1728
                     /label=BBa_B0012
     misc_feature    1749..1766
                     /label=C1 OmpR
     misc_feature    1773..1780
                     /label=linker
     promoter        1781..1786
                     /label=-35
     promoter        1804..1809
                     /label=-10
     misc_feature    1777..1777
                     /label=A
     promoter        1737..1814
                     /label=BBa_R0083
     misc_feature    1827..1830
                     /label=conserved
     RBS             1823..1834
                     /label=BBa_B0034
     CDS             1841..2602
                     /label=CFP (LVA)
     misc_feature    2559..2596
                     /label=SsrA
     misc_feature    2597..2602
                     /label=2
     misc_feature    1909..1909
                     /label=A
     misc_feature    1841..2602
                     /label=BBa_E0022
     misc_feature    1841..2602
                     /label=BBa_E0022
     stem_loop       2622..2665
                     /label=stem_loop
     misc_feature    2611..2690
                     /label=BBa_B0010
     terminator      2611..2690
                     /label=BBa_B0010
     stem_loop       2706..2725
                     /label=T7 TE
     misc_feature    2732..2732
                     /label=stop
     polyA_site      2726..2739
                     /label=polya
     misc_feature    2699..2739
                     /label=BBa_B0012
     terminator      2699..2739
                     /label=BBa_B0012
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatgat taaccgtatc cgcgtagtca cgctgttggt aatggtgctg
      121 ggggtattcg cactgttaca gcttatttcc ggcagtctgt ttttttcttc ccttcaccat
      181 agccagaaga gctttgtggt ttccaatcaa ttacgggaac agcagggcga gctgacgtca
      241 acctgggatt taatgctgca aacgcgcatt aacctgagtc gttcagcggt acggatgatg
      301 atggattcct ccaatcaaca aagtaacgcc aaagttgaat tgctcgatag cgccaggaaa
      361 acattggcgc aggcagcgac gcattataaa aaattcaaaa gcatggcacc gttacctgaa
      421 atggtcgcta ccagtcgtaa tattgatgaa aaatataaaa actattacac agcgttaact
      481 gaactgattg attatctcga ttatggcaat actggagctt atttcgctca gccaacccag
      541 ggaatgcaaa atgcaatggg cgaagcgttt gctcagtacg ccctcagcag tgaaaaactg
      601 tatcgcgata tcgtcactga caacgcagat gattaccgat ttgcccagtg gcaactggcg
      661 gttatcgcgc tggtggtggt attgattctg ctggtggcgt ggtacggcat tcgccgtatg
      721 ttgcttactc cgctggcaaa aattattgct cacattcgcg aaatcgccgg tggtaacctg
      781 gcgaataccc tgaccattga cgggcgcagt gaaatgggcg acctggcgca gagcgtttca
      841 catatggcgg ctggtgttaa gcaactggcg gatgaccgca cgctgctgat ggcgggggta
      901 agtcacgact tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat gatgagcgag
      961 caggatggct atctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt
     1021 gagcagttta tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctt
     1081 aatgcagtac tcggtgaggt gattgctgcc gaaagtggct atgagcggga aattgaaacc
     1141 gcgctttacc ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa acgcgcggtg
     1201 gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct ggatcaaagt cagcagcgga
     1261 acggagccga atcgcgcctg gttccaggtg gaagatgacg gtccgggaat tgcgccggaa
     1321 caacgtaagc acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc
     1381 acgggattag ggctggcaat tgtgcagcgt atcgtggata accataacgg gatgctggag
     1441 cttggcacca gcgagcgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg
     1501 cgggcgcagg gcacgacaaa agaaggggct gcaaacgacg aaaactacgc tttagtagct
     1561 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     1621 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1681 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtccc
     1741 ttgcatttac attttgaaac atctatagcg atctcttgag ttggattatt ctgcattttt
     1801 ggggagaatg gacttactag agaaagagga gaaatactag atggtgagca agggcgagga
     1861 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa
     1921 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     1981 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg
     2041 gggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     2101 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     2161 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     2221 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat
     2281 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa
     2341 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     2401 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     2461 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     2521 cgccgggatc actctcggca tggacgagct gtacaagagg cctgctgcaa acgacgaaaa
     2581 ctacgcttta gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt
     2641 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
     2701 acactggctc accttcgggt gggcctttct gcgtttata
//