LOCUS       BBa_I3312               2769 bp    DNA     linear       22-Nov-2024
DEFINITION  Test: Tar-EnvZ attractant receiver (I3302.E0422)
ACCESSION   BBa_I3312
VERSION     BBa_I3312.1  
FEATURES             Location/Qualifiers
     promoter        35..40
                     /label=-10
     promoter        15..20
                     /label=-35
     promoter        1..49
                     /label=BBa_R1075
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    498..499
                     /label=A
     misc_feature    1528..1560
                     /label=LVA
     CDS             76..840
                     /label=Tar (front)
     CDS             847..1527
                     /label=Envz (back)
     misc_feature    1567..1591
                     /label=Help:Barcodes
     CDS             76..1566
                     /label=BBa_C0082
     stem_loop       1611..1654
                     /label=stem_loop
     misc_feature    1600..1679
                     /label=BBa_B0010
     terminator      1600..1679
                     /label=BBa_B0010
     stem_loop       1695..1714
                     /label=T7 TE
     misc_feature    1721..1721
                     /label=stop
     polyA_site      1715..1728
                     /label=polya
     misc_feature    1688..1728
                     /label=BBa_B0012
     terminator      1688..1728
                     /label=BBa_B0012
     misc_feature    1747..1764
                     /label=F1 OmpR
     misc_feature    1767..1784
                     /label=F2 OmpR
     misc_feature    1787..1804
                     /label=F3 OmpR
     promoter        1809..1814
                     /label=-35
     promoter        1824..1829
                     /label=-10
     promoter        1737..1844
                     /label=BBa_R0084
     misc_feature    1857..1860
                     /label=conserved
     RBS             1853..1864
                     /label=BBa_B0034
     CDS             1871..2632
                     /label=CFP (LVA)
     misc_feature    2589..2626
                     /label=SsrA
     misc_feature    2627..2632
                     /label=2
     misc_feature    1939..1939
                     /label=A
     misc_feature    1871..2632
                     /label=BBa_E0022
     misc_feature    1871..2632
                     /label=BBa_E0022
     stem_loop       2652..2695
                     /label=stem_loop
     misc_feature    2641..2720
                     /label=BBa_B0010
     terminator      2641..2720
                     /label=BBa_B0010
     stem_loop       2736..2755
                     /label=T7 TE
     misc_feature    2762..2762
                     /label=stop
     polyA_site      2756..2769
                     /label=polya
     misc_feature    2729..2769
                     /label=BBa_B0012
     terminator      2729..2769
                     /label=BBa_B0012
ORIGIN
        1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa
       61 gaggagaaat actagatgat taaccgtatc cgcgtagtca cgctgttggt aatggtgctg
      121 ggggtattcg cactgttaca gcttatttcc ggcagtctgt ttttttcttc ccttcaccat
      181 agccagaaga gctttgtggt ttccaatcaa ttacgggaac agcagggcga gctgacgtca
      241 acctgggatt taatgctgca aacgcgcatt aacctgagtc gttcagcggt acggatgatg
      301 atggattcct ccaatcaaca aagtaacgcc aaagttgaat tgctcgatag cgccaggaaa
      361 acattggcgc aggcagcgac gcattataaa aaattcaaaa gcatggcacc gttacctgaa
      421 atggtcgcta ccagtcgtaa tattgatgaa aaatataaaa actattacac agcgttaact
      481 gaactgattg attatctcga ttatggcaat actggagctt atttcgctca gccaacccag
      541 ggaatgcaaa atgcaatggg cgaagcgttt gctcagtacg ccctcagcag tgaaaaactg
      601 tatcgcgata tcgtcactga caacgcagat gattaccgat ttgcccagtg gcaactggcg
      661 gttatcgcgc tggtggtggt attgattctg ctggtggcgt ggtacggcat tcgccgtatg
      721 ttgcttactc cgctggcaaa aattattgct cacattcgcg aaatcgccgg tggtaacctg
      781 gcgaataccc tgaccattga cgggcgcagt gaaatgggcg acctggcgca gagcgtttca
      841 catatggcgg ctggtgttaa gcaactggcg gatgaccgca cgctgctgat ggcgggggta
      901 agtcacgact tgcgcacgcc gctgacgcgt attcgcctgg cgactgagat gatgagcgag
      961 caggatggct atctggcaga atcgatcaat aaagatatcg aagagtgcaa cgccatcatt
     1021 gagcagttta tcgactacct gcgcaccggg caggagatgc cgatggaaat ggcggatctt
     1081 aatgcagtac tcggtgaggt gattgctgcc gaaagtggct atgagcggga aattgaaacc
     1141 gcgctttacc ccggcagcat tgaagtgaaa atgcacccgc tgtcgatcaa acgcgcggtg
     1201 gcgaatatgg tggtcaacgc cgcccgttat ggcaatggct ggatcaaagt cagcagcgga
     1261 acggagccga atcgcgcctg gttccaggtg gaagatgacg gtccgggaat tgcgccggaa
     1321 caacgtaagc acctgttcca gccgtttgtc cgcggcgaca gtgcgcgcac cattagcggc
     1381 acgggattag ggctggcaat tgtgcagcgt atcgtggata accataacgg gatgctggag
     1441 cttggcacca gcgagcgggg cgggctttcc attcgcgcct ggctgccagt gccggtaacg
     1501 cgggcgcagg gcacgacaaa agaaggggct gcaaacgacg aaaactacgc tttagtagct
     1561 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     1621 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1681 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagctta
     1741 aattttactt ttggttacat attttttctt tttgaaacca aatctttatc tttgtagcac
     1801 tttcacggta gcgaaacgtt agtttgaatg gaaagatgcc tgcatactag agaaagagga
     1861 gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     1921 cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     1981 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2041 ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga
     2101 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     2161 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     2221 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     2281 cctggggcac aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa
     2341 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt
     2401 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     2461 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     2521 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     2581 gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat aatactagag
     2641 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     2701 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     2761 gcgtttata
//