LOCUS BBa_I3420 10225 bp DNA linear 22-Dec-2024 DEFINITION Test: Switch functionality and bistability (I3410.E0422.I3411.E0432 .I3100.I3400.I3101.I3400.I3102.I3 ACCESSION BBa_I3420 VERSION BBa_I3420.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 814..819 /label=2 CDS 76..819 /label=cI HK022 misc_feature 781..813 /label=SsrA misc_feature 76..83 /label=OR3 partial misc_feature 76..819 /label=BBa_C0050 misc_feature 820..844 /label=Help:Barcodes CDS 76..819 /label=BBa_C0050 misc_feature 857..860 /label=conserved RBS 853..864 /label=BBa_B0034 CDS 871..1632 /label=CFP (LVA) misc_feature 1589..1626 /label=SsrA misc_feature 1627..1632 /label=2 misc_feature 939..939 /label=A misc_feature 871..1632 /label=BBa_E0022 misc_feature 871..1632 /label=BBa_E0022 stem_loop 1652..1695 /label=stem_loop misc_feature 1641..1720 /label=BBa_B0010 terminator 1641..1720 /label=BBa_B0010 stem_loop 1736..1755 /label=T7 TE misc_feature 1762..1762 /label=stop polyA_site 1756..1769 /label=polya misc_feature 1729..1769 /label=BBa_B0012 terminator 1729..1769 /label=BBa_B0012 promoter 1785..1790 /label=-35 RBS 1790..1804 /label=OR2 promoter 1807..1812 /label=-10 RBS 1814..1828 /label=OR1 misc_feature 1820..1820 /label=putative misc_feature 1778..1832 /label=BBa_R0050 promoter 1778..1832 /label=BBa_R0050 misc_feature 1845..1848 /label=conserved RBS 1841..1852 /label=BBa_B0034 CDS 1862..2569 /label=cI lambda misc_feature 2570..2602 /label=LVA misc_feature 2609..2633 /label=Help:Barcodes CDS 1859..2608 /label=BBa_C0051 misc_feature 2646..2649 /label=conserved RBS 2642..2653 /label=BBa_B0034 CDS 2660..3421 /label=YFP (LVA) misc_feature 3416..3421 /label=2 misc_feature 3377..3415 /label=SsrA misc_feature 2901..2901 /label=A (Q->L) misc_feature 2660..3421 /label=BBa_E0032 misc_feature 2660..3421 /label=BBa_E0032 stem_loop 3441..3484 /label=stem_loop misc_feature 3430..3509 /label=BBa_B0010 terminator 3430..3509 /label=BBa_B0010 stem_loop 3525..3544 /label=T7 TE misc_feature 3551..3551 /label=stop polyA_site 3545..3558 /label=polya misc_feature 3518..3558 /label=BBa_B0012 terminator 3518..3558 /label=BBa_B0012 promoter 3601..3606 /label=-10 promoter 3581..3586 /label=-35 promoter 3567..3615 /label=BBa_R1075 misc_feature 3628..3631 /label=conserved RBS 3624..3635 /label=BBa_B0034 misc_feature 4731..4769 /label=LVA CDS 3642..4769 /label=lacI-LVA misc_feature 3642..4769 /label=BBa_C0012 misc_feature 4770..4794 /label=Help:Barcodes CDS 3642..4769 /label=BBa_C0012 stem_loop 4814..4857 /label=stem_loop misc_feature 4803..4882 /label=BBa_B0010 terminator 4803..4882 /label=BBa_B0010 stem_loop 4898..4917 /label=T7 TE misc_feature 4924..4924 /label=stop polyA_site 4918..4931 /label=polya misc_feature 4891..4931 /label=BBa_B0012 terminator 4891..4931 /label=BBa_B0012 CDS 4940..5027 /label=end of LacI coding region (inactive) misc_feature 4940..5139 /label=BBa_R0010 misc_feature 5028..5065 /label=CAP binding site promoter 5076..5081 /label=-35 promoter 5100..5105 /label=-10 misc_feature 5105..5139 /label=LacI binding site misc_feature 5112..5112 /label=start promoter 4940..5139 /label=BBa_R0010 misc_feature 5152..5155 /label=conserved RBS 5148..5159 /label=BBa_B0034 misc_feature 5904..5909 /label=2 CDS 5166..5909 /label=cI HK022 misc_feature 5871..5903 /label=SsrA misc_feature 5166..5173 /label=OR3 partial misc_feature 5166..5909 /label=BBa_C0050 misc_feature 5910..5934 /label=Help:Barcodes CDS 5166..5909 /label=BBa_C0050 stem_loop 5954..5997 /label=stem_loop misc_feature 5943..6022 /label=BBa_B0010 terminator 5943..6022 /label=BBa_B0010 stem_loop 6038..6057 /label=T7 TE misc_feature 6064..6064 /label=stop polyA_site 6058..6071 /label=polya misc_feature 6031..6071 /label=BBa_B0012 terminator 6031..6071 /label=BBa_B0012 promoter 6114..6119 /label=-10 promoter 6094..6099 /label=-35 promoter 6080..6128 /label=BBa_R1075 misc_feature 6141..6144 /label=conserved RBS 6137..6148 /label=BBa_B0034 CDS 6158..6774 /label=tetR misc_feature 6775..6808 /label=SsrA misc_feature 6815..6839 /label=Help:Barcodes CDS 6155..6814 /label=BBa_C0040 stem_loop 6859..6902 /label=stem_loop misc_feature 6848..6927 /label=BBa_B0010 terminator 6848..6927 /label=BBa_B0010 stem_loop 6943..6962 /label=T7 TE misc_feature 6969..6969 /label=stop polyA_site 6963..6976 /label=polya misc_feature 6936..6976 /label=BBa_B0012 terminator 6936..6976 /label=BBa_B0012 misc_feature 6985..7003 /label=TetR 1 misc_feature 6985..7038 /label=BBa_R0040 promoter 7004..7009 /label=-35 misc_feature 7010..7028 /label=TetR 2 promoter 7027..7032 /label=-10 promoter 6985..7038 /label=BBa_R0040 misc_feature 7051..7054 /label=conserved RBS 7047..7058 /label=BBa_B0034 misc_feature 7803..7808 /label=2 CDS 7065..7808 /label=cI HK022 misc_feature 7770..7802 /label=SsrA misc_feature 7065..7072 /label=OR3 partial misc_feature 7065..7808 /label=BBa_C0050 misc_feature 7809..7833 /label=Help:Barcodes CDS 7065..7808 /label=BBa_C0050 stem_loop 7853..7896 /label=stem_loop misc_feature 7842..7921 /label=BBa_B0010 terminator 7842..7921 /label=BBa_B0010 stem_loop 7937..7956 /label=T7 TE misc_feature 7963..7963 /label=stop polyA_site 7957..7970 /label=polya misc_feature 7930..7970 /label=BBa_B0012 terminator 7930..7970 /label=BBa_B0012 promoter 8013..8018 /label=-10 promoter 7993..7998 /label=-35 promoter 7979..8027 /label=BBa_R1075 misc_feature 8040..8043 /label=conserved RBS 8036..8047 /label=BBa_B0034 CDS 8054..8929 /label=araC misc_feature 8930..8962 /label=LVA misc_feature 8969..8993 /label=Help:Barcodes CDS 8054..8968 /label=BBa_C0080 stem_loop 9013..9056 /label=stem_loop misc_feature 9002..9081 /label=BBa_B0010 terminator 9002..9081 /label=BBa_B0010 stem_loop 9097..9116 /label=T7 TE misc_feature 9123..9123 /label=stop polyA_site 9117..9130 /label=polya misc_feature 9090..9130 /label=BBa_B0012 terminator 9090..9130 /label=BBa_B0012 misc_feature 9142..9167 /label=c-amp2 misc_feature 9144..9182 /label=araO1 misc_feature 9181..9210 /label=c-amp1 misc_feature 9211..9239 /label=ara1 and ara2 promoter 9251..9256 /label=-35 promoter 9274..9279 /label=-10 promoter 9139..9287 /label=BBa_R0080 misc_feature 9300..9303 /label=conserved RBS 9296..9307 /label=BBa_B0034 CDS 9317..10024 /label=cI lambda misc_feature 10025..10057 /label=LVA misc_feature 10064..10088 /label=Help:Barcodes CDS 9314..10063 /label=BBa_C0051 stem_loop 10108..10151 /label=stem_loop misc_feature 10097..10176 /label=BBa_B0010 terminator 10097..10176 /label=BBa_B0010 stem_loop 10192..10211 /label=T7 TE misc_feature 10218..10218 /label=stop polyA_site 10212..10225 /label=polya misc_feature 10185..10225 /label=BBa_B0012 terminator 10185..10225 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt tcaacagaaa gagcgtgaaa ctttctcgca gaggcttgcg 121 ctggcctgtg ataaagcggg attacctttg catggtaggc aggctgattt agctgtcagg 181 cttaaggtca caccaaaagc cattagtaaa tggttcaacg gggagtcaat accaagaaaa 241 gacaagatgg aatctctggc ttcggtgctg ggaactactg ctgcatatct gcatggctat 301 gctgatgatg acggtatcac ggtaaatcat ctatcaagat caaatgatta ttatcgtgtt 361 gatgtattgg atgttcaggc gagcgccggg ccaggaacca tggtttccaa tgaatttata 421 gaaaagataa gagcaattga atatacgacc gagcaggcaa gaattttatt taatggaagg 481 ccacaggaaa gcgtaaaagt catcacggtt cgcggtgaca gcatggaggg aaccatcaat 541 ccgggagatg agatctttgt tgatgtatcc ataacctgtt ttgatggcga tggcatttat 601 gtgtttgtat acgggaaaac aatgcacgtt aagcgcctgc aaatgcaaaa gaacaggctt 661 gccgtcatct ctgacaatgc cgcttatgat cgatggtaca tagaagaagg tgaagaagag 721 caacttcaca ttctagccaa agtcctcatt aggcagtcaa tcgattacaa gcgattcgga 781 gctgcaaacg acgaaaacta cgctttagta gcttaataac cctgatagtg ctagtgtaga 841 tccctactag agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc 901 ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg 961 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 1021 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag 1081 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 1141 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 1201 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 1261 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat cagccacaac 1321 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac 1381 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 1441 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 1501 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 1561 actctcggca tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta 1621 gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1681 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1741 accttcgggt gggcctttct gcgtttatat actagagtta ggtattgact gtactatcag 1801 ttccgtcata atatgaacca taagttcacc actactagag aaagaggaga aatactagat 1861 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 1921 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 1981 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 2041 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 2101 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 2161 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac 2221 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 2281 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 2341 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 2401 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 2461 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 2521 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga 2581 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga 2641 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 2701 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 2761 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 2821 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg 2881 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt 2941 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 3001 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 3061 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 3121 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 3181 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 3241 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 3301 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 3361 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata 3421 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3481 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3541 ggcctttctg cgtttatata ctagagttaa atttcctctt ttcaggccgg aataactccc 3601 tataatgcgc caccatacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag 3661 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 3721 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 3781 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 3841 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 3901 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 3961 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 4021 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 4081 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 4141 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 4201 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 4261 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 4321 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 4381 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 4441 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 4501 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 4561 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 4621 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 4681 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg 4741 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 4801 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 4861 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 4921 ctgcgtttat atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 4981 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 5041 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 5101 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat 5161 actagatggt tcaacagaaa gagcgtgaaa ctttctcgca gaggcttgcg ctggcctgtg 5221 ataaagcggg attacctttg catggtaggc aggctgattt agctgtcagg cttaaggtca 5281 caccaaaagc cattagtaaa tggttcaacg gggagtcaat accaagaaaa gacaagatgg 5341 aatctctggc ttcggtgctg ggaactactg ctgcatatct gcatggctat gctgatgatg 5401 acggtatcac ggtaaatcat ctatcaagat caaatgatta ttatcgtgtt gatgtattgg 5461 atgttcaggc gagcgccggg ccaggaacca tggtttccaa tgaatttata gaaaagataa 5521 gagcaattga atatacgacc gagcaggcaa gaattttatt taatggaagg ccacaggaaa 5581 gcgtaaaagt catcacggtt cgcggtgaca gcatggaggg aaccatcaat ccgggagatg 5641 agatctttgt tgatgtatcc ataacctgtt ttgatggcga tggcatttat gtgtttgtat 5701 acgggaaaac aatgcacgtt aagcgcctgc aaatgcaaaa gaacaggctt gccgtcatct 5761 ctgacaatgc cgcttatgat cgatggtaca tagaagaagg tgaagaagag caacttcaca 5821 ttctagccaa agtcctcatt aggcagtcaa tcgattacaa gcgattcgga gctgcaaacg 5881 acgaaaacta cgctttagta gcttaataac cctgatagtg ctagtgtaga tccctactag 5941 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 6001 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 6061 ctgcgtttat atactagagt taaatttcct cttttcaggc cggaataact ccctataatg 6121 cgccaccata ctagagaaag aggagaaata ctagatgtcc agattagata aaagtaaagt 6181 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa 6241 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg 6301 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc 6361 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc 6421 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca 6481 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa 6541 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca 6601 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt 6661 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg 6721 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc 6781 aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact 6841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 6901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 6961 cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt 7021 gatagagata ctgagcacta ctagagaaag aggagaaata ctagatggtt caacagaaag 7081 agcgtgaaac tttctcgcag aggcttgcgc tggcctgtga taaagcggga ttacctttgc 7141 atggtaggca ggctgattta gctgtcaggc ttaaggtcac accaaaagcc attagtaaat 7201 ggttcaacgg ggagtcaata ccaagaaaag acaagatgga atctctggct tcggtgctgg 7261 gaactactgc tgcatatctg catggctatg ctgatgatga cggtatcacg gtaaatcatc 7321 tatcaagatc aaatgattat tatcgtgttg atgtattgga tgttcaggcg agcgccgggc 7381 caggaaccat ggtttccaat gaatttatag aaaagataag agcaattgaa tatacgaccg 7441 agcaggcaag aattttattt aatggaaggc cacaggaaag cgtaaaagtc atcacggttc 7501 gcggtgacag catggaggga accatcaatc cgggagatga gatctttgtt gatgtatcca 7561 taacctgttt tgatggcgat ggcatttatg tgtttgtata cgggaaaaca atgcacgtta 7621 agcgcctgca aatgcaaaag aacaggcttg ccgtcatctc tgacaatgcc gcttatgatc 7681 gatggtacat agaagaaggt gaagaagagc aacttcacat tctagccaaa gtcctcatta 7741 ggcagtcaat cgattacaag cgattcggag ctgcaaacga cgaaaactac gctttagtag 7801 cttaataacc ctgatagtgc tagtgtagat ccctactaga gccaggcatc aaataaaacg 7861 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 7921 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt 7981 aaatttcctc ttttcaggcc ggaataactc cctataatgc gccaccatac tagagaaaga 8041 ggagaaatac tagatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa 8101 cgcccatctg gtggcgggtt taacgccgat tgaggccaat ggttatctcg atttttttat 8161 cgaccgaccg ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggggt 8221 ggtgaaaaat cagggacgag aatttgtctg ccgaccgggt gatattttgc tgttcccgcc 8281 aggagagatt catcactacg gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt 8341 ttactttcgt ccgcgcgcct actggcatga atggcttaac tggccgtcaa tatttgccaa 8401 tacgggtttc tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca 8461 aatcattaac gccgggcaag gggaagggcg ctattcggag ctgctggcga taaatctgct 8521 tgagcaattg ttactgcggc gcatggaagc gattaacgag tcgctccatc caccgatgga 8581 taatcgggta cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gcaattttga 8641 tatcgccagc gtcgcacagc atgtttgcct gtcgccgtcg cgtctgtcac atcttttccg 8701 ccagcagtta gggattagcg tcttaagctg gcgcgaggac caacgcatca gccaggcgaa 8761 gctgcttttg agcactaccc ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga 8821 cgatcaactc tatttctcgc gagtatttaa aaaatgcacc ggggccagcc cgagcgagtt 8881 ccgtgccggt tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcag ctgcaaacga 8941 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga 9001 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 9061 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 9121 tgcgtttata tactagaggc gtaacaaaag tgtctataat cacggcagaa aagtccacat 9181 tgattatttg cacggcgtca cactttgcta tgccatagca tttttatcca taagattagc 9241 ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccattac tagagaaaga 9301 ggagaaatac tagatgagca caaaaaagaa accattaaca caagagcagc ttgaggacgc 9361 acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc 9421 tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta atggcatcaa 9481 tgcattaaat gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga 9541 atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta gtatgcagcc 9601 gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag ggatgttctc 9661 acctgagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa 9721 agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg caccaacagg 9781 ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc aggctgttga 9841 gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca agaaactgat 9901 cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa tgatcccatg 9961 caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg aagagacgtt 10021 tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat agtgctagtg 10081 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 10141 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 10201 tcgggtgggc ctttctgcgt ttata //