LOCUS BBa_I3424 10074 bp DNA linear 29-Nov-2024 DEFINITION Test: Switch response to quorum (I3410.E0422.I3411.E0432.I3100.I340 0.I3101.I3400.I3510.I3401) ACCESSION BBa_I3424 VERSION BBa_I3424.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 814..819 /label=2 CDS 76..819 /label=cI HK022 misc_feature 781..813 /label=SsrA misc_feature 76..83 /label=OR3 partial misc_feature 76..819 /label=BBa_C0050 misc_feature 820..844 /label=Help:Barcodes CDS 76..819 /label=BBa_C0050 misc_feature 857..860 /label=conserved RBS 853..864 /label=BBa_B0034 CDS 871..1632 /label=CFP (LVA) misc_feature 1589..1626 /label=SsrA misc_feature 1627..1632 /label=2 misc_feature 939..939 /label=A misc_feature 871..1632 /label=BBa_E0022 misc_feature 871..1632 /label=BBa_E0022 stem_loop 1652..1695 /label=stem_loop misc_feature 1641..1720 /label=BBa_B0010 terminator 1641..1720 /label=BBa_B0010 stem_loop 1736..1755 /label=T7 TE misc_feature 1762..1762 /label=stop polyA_site 1756..1769 /label=polya misc_feature 1729..1769 /label=BBa_B0012 terminator 1729..1769 /label=BBa_B0012 promoter 1785..1790 /label=-35 RBS 1790..1804 /label=OR2 promoter 1807..1812 /label=-10 RBS 1814..1828 /label=OR1 misc_feature 1820..1820 /label=putative misc_feature 1778..1832 /label=BBa_R0050 promoter 1778..1832 /label=BBa_R0050 misc_feature 1845..1848 /label=conserved RBS 1841..1852 /label=BBa_B0034 CDS 1862..2569 /label=cI lambda misc_feature 2570..2602 /label=LVA misc_feature 2609..2633 /label=Help:Barcodes CDS 1859..2608 /label=BBa_C0051 misc_feature 2646..2649 /label=conserved RBS 2642..2653 /label=BBa_B0034 CDS 2660..3421 /label=YFP (LVA) misc_feature 3416..3421 /label=2 misc_feature 3377..3415 /label=SsrA misc_feature 2901..2901 /label=A (Q->L) misc_feature 2660..3421 /label=BBa_E0032 misc_feature 2660..3421 /label=BBa_E0032 stem_loop 3441..3484 /label=stem_loop misc_feature 3430..3509 /label=BBa_B0010 terminator 3430..3509 /label=BBa_B0010 stem_loop 3525..3544 /label=T7 TE misc_feature 3551..3551 /label=stop polyA_site 3545..3558 /label=polya misc_feature 3518..3558 /label=BBa_B0012 terminator 3518..3558 /label=BBa_B0012 promoter 3601..3606 /label=-10 promoter 3581..3586 /label=-35 promoter 3567..3615 /label=BBa_R1075 misc_feature 3628..3631 /label=conserved RBS 3624..3635 /label=BBa_B0034 misc_feature 4731..4769 /label=LVA CDS 3642..4769 /label=lacI-LVA misc_feature 3642..4769 /label=BBa_C0012 misc_feature 4770..4794 /label=Help:Barcodes CDS 3642..4769 /label=BBa_C0012 stem_loop 4814..4857 /label=stem_loop misc_feature 4803..4882 /label=BBa_B0010 terminator 4803..4882 /label=BBa_B0010 stem_loop 4898..4917 /label=T7 TE misc_feature 4924..4924 /label=stop polyA_site 4918..4931 /label=polya misc_feature 4891..4931 /label=BBa_B0012 terminator 4891..4931 /label=BBa_B0012 CDS 4940..5027 /label=end of LacI coding region (inactive) misc_feature 4940..5139 /label=BBa_R0010 misc_feature 5028..5065 /label=CAP binding site promoter 5076..5081 /label=-35 promoter 5100..5105 /label=-10 misc_feature 5105..5139 /label=LacI binding site misc_feature 5112..5112 /label=start promoter 4940..5139 /label=BBa_R0010 misc_feature 5152..5155 /label=conserved RBS 5148..5159 /label=BBa_B0034 misc_feature 5904..5909 /label=2 CDS 5166..5909 /label=cI HK022 misc_feature 5871..5903 /label=SsrA misc_feature 5166..5173 /label=OR3 partial misc_feature 5166..5909 /label=BBa_C0050 misc_feature 5910..5934 /label=Help:Barcodes CDS 5166..5909 /label=BBa_C0050 stem_loop 5954..5997 /label=stem_loop misc_feature 5943..6022 /label=BBa_B0010 terminator 5943..6022 /label=BBa_B0010 stem_loop 6038..6057 /label=T7 TE misc_feature 6064..6064 /label=stop polyA_site 6058..6071 /label=polya misc_feature 6031..6071 /label=BBa_B0012 terminator 6031..6071 /label=BBa_B0012 promoter 6114..6119 /label=-10 promoter 6094..6099 /label=-35 promoter 6080..6128 /label=BBa_R1075 misc_feature 6141..6144 /label=conserved RBS 6137..6148 /label=BBa_B0034 CDS 6158..6774 /label=tetR misc_feature 6775..6808 /label=SsrA misc_feature 6815..6839 /label=Help:Barcodes CDS 6155..6814 /label=BBa_C0040 stem_loop 6859..6902 /label=stem_loop misc_feature 6848..6927 /label=BBa_B0010 terminator 6848..6927 /label=BBa_B0010 stem_loop 6943..6962 /label=T7 TE misc_feature 6969..6969 /label=stop polyA_site 6963..6976 /label=polya misc_feature 6936..6976 /label=BBa_B0012 terminator 6936..6976 /label=BBa_B0012 misc_feature 6985..7003 /label=TetR 1 misc_feature 6985..7038 /label=BBa_R0040 promoter 7004..7009 /label=-35 misc_feature 7010..7028 /label=TetR 2 promoter 7027..7032 /label=-10 promoter 6985..7038 /label=BBa_R0040 misc_feature 7051..7054 /label=conserved RBS 7047..7058 /label=BBa_B0034 misc_feature 7803..7808 /label=2 CDS 7065..7808 /label=cI HK022 misc_feature 7770..7802 /label=SsrA misc_feature 7065..7072 /label=OR3 partial misc_feature 7065..7808 /label=BBa_C0050 misc_feature 7809..7833 /label=Help:Barcodes CDS 7065..7808 /label=BBa_C0050 stem_loop 7853..7896 /label=stem_loop misc_feature 7842..7921 /label=BBa_B0010 terminator 7842..7921 /label=BBa_B0010 stem_loop 7937..7956 /label=T7 TE misc_feature 7963..7963 /label=stop polyA_site 7957..7970 /label=polya misc_feature 7930..7970 /label=BBa_B0012 terminator 7930..7970 /label=BBa_B0012 misc_feature 7979..7998 /label=LuxR/HSL promoter 8093..8100 /label=-10 promoter 8067..8072 /label=-35 misc_feature 8106..8106 /label=start misc_feature 8108..8127 /label=Putative LuxR/HSL misc_feature 7979..8129 /label=BBa_R0063 promoter 7979..8129 /label=BBa_R0063 misc_feature 8142..8145 /label=conserved RBS 8138..8149 /label=BBa_B0034 misc_feature 8156..8157 /label=prefix misc_feature 8329..8329 /label=T misc_feature 8647..8647 /label=A CDS 8156..8905 /label=luxR misc_feature 8156..8911 /label=BBa_C0062 misc_feature 8912..8936 /label=Help:Barcodes CDS 8156..8911 /label=BBa_C0062 stem_loop 8956..8999 /label=stem_loop misc_feature 8945..9024 /label=BBa_B0010 terminator 8945..9024 /label=BBa_B0010 stem_loop 9040..9059 /label=T7 TE misc_feature 9066..9066 /label=stop polyA_site 9060..9073 /label=polya misc_feature 9033..9073 /label=BBa_B0012 terminator 9033..9073 /label=BBa_B0012 misc_feature 9082..9101 /label=LuxR/HSL promoter 9101..9106 /label=-35 promoter 9123..9128 /label=-10 misc_feature 9134..9134 /label=start misc_feature 9082..9136 /label=BBa_R0062 promoter 9082..9136 /label=BBa_R0062 misc_feature 9149..9152 /label=conserved RBS 9145..9156 /label=BBa_B0034 CDS 9166..9873 /label=cI lambda misc_feature 9874..9906 /label=LVA misc_feature 9913..9937 /label=Help:Barcodes CDS 9163..9912 /label=BBa_C0051 stem_loop 9957..10000 /label=stem_loop misc_feature 9946..10025 /label=BBa_B0010 terminator 9946..10025 /label=BBa_B0010 stem_loop 10041..10060 /label=T7 TE misc_feature 10067..10067 /label=stop polyA_site 10061..10074 /label=polya misc_feature 10034..10074 /label=BBa_B0012 terminator 10034..10074 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa 61 gaggagaaat actagatggt tcaacagaaa gagcgtgaaa ctttctcgca gaggcttgcg 121 ctggcctgtg ataaagcggg attacctttg catggtaggc aggctgattt agctgtcagg 181 cttaaggtca caccaaaagc cattagtaaa tggttcaacg gggagtcaat accaagaaaa 241 gacaagatgg aatctctggc ttcggtgctg ggaactactg ctgcatatct gcatggctat 301 gctgatgatg acggtatcac ggtaaatcat ctatcaagat caaatgatta ttatcgtgtt 361 gatgtattgg atgttcaggc gagcgccggg ccaggaacca tggtttccaa tgaatttata 421 gaaaagataa gagcaattga atatacgacc gagcaggcaa gaattttatt taatggaagg 481 ccacaggaaa gcgtaaaagt catcacggtt cgcggtgaca gcatggaggg aaccatcaat 541 ccgggagatg agatctttgt tgatgtatcc ataacctgtt ttgatggcga tggcatttat 601 gtgtttgtat acgggaaaac aatgcacgtt aagcgcctgc aaatgcaaaa gaacaggctt 661 gccgtcatct ctgacaatgc cgcttatgat cgatggtaca tagaagaagg tgaagaagag 721 caacttcaca ttctagccaa agtcctcatt aggcagtcaa tcgattacaa gcgattcgga 781 gctgcaaacg acgaaaacta cgctttagta gcttaataac cctgatagtg ctagtgtaga 841 tccctactag agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc 901 ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg 961 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 1021 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag 1081 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 1141 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 1201 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 1261 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat cagccacaac 1321 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac 1381 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 1441 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 1501 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 1561 actctcggca tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta 1621 gtagcttaat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 1681 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 1741 accttcgggt gggcctttct gcgtttatat actagagtta ggtattgact gtactatcag 1801 ttccgtcata atatgaacca taagttcacc actactagag aaagaggaga aatactagat 1861 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc 1921 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat 1981 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta 2041 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat 2101 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga 2161 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac 2221 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc 2281 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt 2341 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg 2401 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca 2461 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc 2521 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga 2581 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga 2641 gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 2701 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 2761 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 2821 gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat gcttcgcccg 2881 ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg aaggctacgt 2941 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 3001 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 3061 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 3121 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 3181 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 3241 gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga 3301 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 3361 ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata 3421 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 3481 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 3541 ggcctttctg cgtttatata ctagagttaa atttcctctt ttcaggccgg aataactccc 3601 tataatgcgc caccatacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag 3661 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg 3721 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg 3781 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga 3841 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta 3901 aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg 3961 tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca 4021 ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc 4081 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg 4141 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc 4201 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat 4261 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt 4321 ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg 4381 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg 4441 ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc 4501 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct 4561 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg 4621 tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 4681 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg 4741 acgaaaacta cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag 4801 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 4861 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 4921 ctgcgtttat atactagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat 4981 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt 5041 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt 5101 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat 5161 actagatggt tcaacagaaa gagcgtgaaa ctttctcgca gaggcttgcg ctggcctgtg 5221 ataaagcggg attacctttg catggtaggc aggctgattt agctgtcagg cttaaggtca 5281 caccaaaagc cattagtaaa tggttcaacg gggagtcaat accaagaaaa gacaagatgg 5341 aatctctggc ttcggtgctg ggaactactg ctgcatatct gcatggctat gctgatgatg 5401 acggtatcac ggtaaatcat ctatcaagat caaatgatta ttatcgtgtt gatgtattgg 5461 atgttcaggc gagcgccggg ccaggaacca tggtttccaa tgaatttata gaaaagataa 5521 gagcaattga atatacgacc gagcaggcaa gaattttatt taatggaagg ccacaggaaa 5581 gcgtaaaagt catcacggtt cgcggtgaca gcatggaggg aaccatcaat ccgggagatg 5641 agatctttgt tgatgtatcc ataacctgtt ttgatggcga tggcatttat gtgtttgtat 5701 acgggaaaac aatgcacgtt aagcgcctgc aaatgcaaaa gaacaggctt gccgtcatct 5761 ctgacaatgc cgcttatgat cgatggtaca tagaagaagg tgaagaagag caacttcaca 5821 ttctagccaa agtcctcatt aggcagtcaa tcgattacaa gcgattcgga gctgcaaacg 5881 acgaaaacta cgctttagta gcttaataac cctgatagtg ctagtgtaga tccctactag 5941 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg 6001 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt 6061 ctgcgtttat atactagagt taaatttcct cttttcaggc cggaataact ccctataatg 6121 cgccaccata ctagagaaag aggagaaata ctagatgtcc agattagata aaagtaaagt 6181 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa 6241 actcgcccag aagctaggtg tagagcagcc tacattgtat tggcatgtaa aaaataagcg 6301 ggctttgctc gacgccttag ccattgagat gttagatagg caccatactc acttttgccc 6361 tttagaaggg gaaagctggc aagatttttt acgtaataac gctaaaagtt ttagatgtgc 6421 tttactaagt catcgcgatg gagcaaaagt acatttaggt acacggccta cagaaaaaca 6481 gtatgaaact ctcgaaaatc aattagcctt tttatgccaa caaggttttt cactagagaa 6541 tgcattatat gcactcagcg ctgtggggca ttttacttta ggttgcgtat tggaagatca 6601 agagcatcaa gtcgctaaag aagaaaggga aacacctact actgatagta tgccgccatt 6661 attacgacaa gctatcgaat tatttgatca ccaaggtgca gagccagcct tcttattcgg 6721 ccttgaattg atcatatgcg gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc 6781 aaacgacgaa aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact 6841 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 6901 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 6961 cctttctgcg tttatatact agagtcccta tcagtgatag agattgacat ccctatcagt 7021 gatagagata ctgagcacta ctagagaaag aggagaaata ctagatggtt caacagaaag 7081 agcgtgaaac tttctcgcag aggcttgcgc tggcctgtga taaagcggga ttacctttgc 7141 atggtaggca ggctgattta gctgtcaggc ttaaggtcac accaaaagcc attagtaaat 7201 ggttcaacgg ggagtcaata ccaagaaaag acaagatgga atctctggct tcggtgctgg 7261 gaactactgc tgcatatctg catggctatg ctgatgatga cggtatcacg gtaaatcatc 7321 tatcaagatc aaatgattat tatcgtgttg atgtattgga tgttcaggcg agcgccgggc 7381 caggaaccat ggtttccaat gaatttatag aaaagataag agcaattgaa tatacgaccg 7441 agcaggcaag aattttattt aatggaaggc cacaggaaag cgtaaaagtc atcacggttc 7501 gcggtgacag catggaggga accatcaatc cgggagatga gatctttgtt gatgtatcca 7561 taacctgttt tgatggcgat ggcatttatg tgtttgtata cgggaaaaca atgcacgtta 7621 agcgcctgca aatgcaaaag aacaggcttg ccgtcatctc tgacaatgcc gcttatgatc 7681 gatggtacat agaagaaggt gaagaagagc aacttcacat tctagccaaa gtcctcatta 7741 ggcagtcaat cgattacaag cgattcggag ctgcaaacga cgaaaactac gctttagtag 7801 cttaataacc ctgatagtgc tagtgtagat ccctactaga gccaggcatc aaataaaacg 7861 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 7921 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac 7981 ctgtacgatc ctacaggtgc ttatgttaag taattgtatt cccagcgata caatagtgtg 8041 acaaaaatcc aatttattag aatcaaatgt caatccatta ccgttttaat gatatataac 8101 acgcaaaact tgcgacaaac aataggtaat actagagaaa gaggagaaat actagatgaa 8161 aaacataaat gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa 8221 taatgatatt aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt 8281 actcgcgatc atttatcctc attctatggt taaatctgat atttcaatcc tagataatta 8341 ccctaaaaaa tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt 8401 agattattct aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt 8461 aaataaaaaa tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt 8521 tagtttccct attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga 8581 aaaagacaac tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt 8641 tccttctcta gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga 8701 tttaaccaaa agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga 8761 tatttcaaaa atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca 8821 aatgaaactc aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc 8881 aattgattgc ccatacttta aaaattaata acactgatag tgctagtgta gatcactact 8941 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 9001 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 9061 ttctgcgttt atatactaga gacctgtagg atcgtacagg tttacgcaag aaaatggttt 9121 gttatagtcg aataaatact agagaaagag gagaaatact agatgagcac aaaaaagaaa 9181 ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag 9241 aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat ggggcagtca 9301 ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc cgcattgctt 9361 gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag agaaatctac 9421 gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga gtaccctgtt 9481 ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac caaaggtgat 9541 gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt 9601 gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga cggaatgtta 9661 attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc cagacttggg 9721 ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt tttacaacca 9781 ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt ggggaaagtt 9841 atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa ctacgcttta 9901 gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa 9961 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 10021 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //