BBa_I3425
type: ComponentDefinition
persistent identity: BBa I3425
display id: BBa_I3425
version: 1
was generated by: iGEM to SBOL conversion
description: Test: Switch aspartate excretion (I3410.I3200.I3411.E0432.I3100.I3400.I3101.I3400.I3102.I3401)
created: 2004-01-28T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: Tests ability of switch to output aspartate given particular inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the AspA attractant sender (I3410.I3200). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and TetR trigger controls the other (I3101.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
star: false
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_R0010
component: BBa_B0012
component: BBa_B0010
component: BBa_C0012
component: BBa_B0034
component: BBa_R1075
component: BBa_C0040
component: BBa_C0050
component: BBa_R0040
component: BBa_C0051
component: BBa_R0080
component: BBa_C0080
component: BBa_R0051
component: BBa_E0032
component: BBa_R0050
component: BBa_C0083
sequence annotation: BBa_R0051
sequence annotation: BBa_B0034
sequence annotation: BBa_C0050
sequence annotation: BBa_C0083
sequence annotation: BBa_R0050
sequence annotation: BBa_C0051
sequence annotation: BBa_E0032
sequence annotation: BBa_B0010

BBa_R0010
type: Component
persistent identity: component1006014
display id: component1006014
version: 1
top level: BBa_I3425
definition: LacI
access: public

BBa_B0012
type: Component
persistent identity: component1005998
display id: component1005998
version: 1
top level: BBa_I3425
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1005988
display id: component1005988
version: 1
top level: BBa_I3425
definition: BBa_B0010
access: public

BBa_C0012
type: Component
persistent identity: component1005981
display id: component1005981
version: 1
top level: BBa_I3425
definition: lacI
access: public

BBa_B0034
type: Component
persistent identity: component1005971
display id: component1005971
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component1005966
display id: component1005966
version: 1
top level: BBa_I3425
definition: const.
access: public

BBa_B0012
type: Component
persistent identity: component1005955
display id: component1005955
version: 1
top level: BBa_I3425
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1005945
display id: component1005945
version: 1
top level: BBa_I3425
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component1006089
display id: component1006089
version: 1
top level: BBa_I3425
definition: BBa_B0010
access: public

BBa_C0040
type: Component
persistent identity: component1006083
display id: component1006083
version: 1
top level: BBa_I3425
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component1006073
display id: component1006073
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component1006068
display id: component1006068
version: 1
top level: BBa_I3425
definition: const.
access: public

BBa_B0012
type: Component
persistent identity: component1006057
display id: component1006057
version: 1
top level: BBa_I3425
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1006047
display id: component1006047
version: 1
top level: BBa_I3425
definition: BBa_B0010
access: public

BBa_C0050
type: Component
persistent identity: component1006039
display id: component1006039
version: 1
top level: BBa_I3425
definition: cI HK022
access: public

BBa_B0034
type: Component
persistent identity: component1006024
display id: component1006024
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component1006171
display id: component1006171
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_R1075
type: Component
persistent identity: component1006166
display id: component1006166
version: 1
top level: BBa_I3425
definition: const.
access: public

BBa_B0012
type: Component
persistent identity: component1006155
display id: component1006155
version: 1
top level: BBa_I3425
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1006145
display id: component1006145
version: 1
top level: BBa_I3425
definition: BBa_B0010
access: public

BBa_C0050
type: Component
persistent identity: component1006137
display id: component1006137
version: 1
top level: BBa_I3425
definition: cI HK022
access: public

BBa_B0034
type: Component
persistent identity: component1006122
display id: component1006122
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1006114
display id: component1006114
version: 1
top level: BBa_I3425
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1006099
display id: component1006099
version: 1
top level: BBa_I3425
definition: BBa_B0012
access: public

BBa_B0012
type: Component
persistent identity: component1006251
display id: component1006251
version: 1
top level: BBa_I3425
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1006241
display id: component1006241
version: 1
top level: BBa_I3425
definition: BBa_B0010
access: public

BBa_C0051
type: Component
persistent identity: component1006235
display id: component1006235
version: 1
top level: BBa_I3425
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component1006225
display id: component1006225
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_R0080
type: Component
persistent identity: component1006220
display id: component1006220
version: 1
top level: BBa_I3425
definition: AraC
access: public

BBa_B0012
type: Component
persistent identity: component1006197
display id: component1006197
version: 1
top level: BBa_I3425
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1006187
display id: component1006187
version: 1
top level: BBa_I3425
definition: BBa_B0010
access: public

BBa_C0080
type: Component
persistent identity: component1006181
display id: component1006181
version: 1
top level: BBa_I3425
definition: araC
access: public

BBa_B0034
type: Component
persistent identity: component1005835
display id: component1005835
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_R0051
type: Component
persistent identity: component1005827
display id: component1005827
version: 1
top level: BBa_I3425
definition: cI lam
access: public

BBa_E0032
type: Component
persistent identity: component1005937
display id: component1005937
version: 1
top level: BBa_I3425
definition: YFP
access: public

BBa_B0034
type: Component
persistent identity: component1005925
display id: component1005925
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_C0051
type: Component
persistent identity: component1005919
display id: component1005919
version: 1
top level: BBa_I3425
definition: cI lam
access: public

BBa_B0034
type: Component
persistent identity: component1005909
display id: component1005909
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_R0050
type: Component
persistent identity: component1005899
display id: component1005899
version: 1
top level: BBa_I3425
definition: cI HK022
access: public

BBa_C0083
type: Component
persistent identity: component1005885
display id: component1005885
version: 1
top level: BBa_I3425
definition: aspA
access: public

BBa_B0034
type: Component
persistent identity: component1005858
display id: component1005858
version: 1
top level: BBa_I3425
definition: BBa_B0034
access: public

BBa_C0050
type: Component
persistent identity: component1005850
display id: component1005850
version: 1
top level: BBa_I3425
definition: cI HK022
access: public

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation1005827
display id: annotation1005827
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005827/range1005827/1
component: BBa_R0051

https://synbiohub.org/public/igem/BBa_I3425/annotation1005827/range1005827/1
type: Range
persistent identity: range1005827
display id: range1005827
version: 1
top level: BBa_I3425
direction: direction/forward
start: 1
end: 49
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1005835
display id: annotation1005835
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005835/range1005835/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1005835/range1005835/1
type: Range
persistent identity: range1005835
display id: range1005835
version: 1
top level: BBa_I3425
direction: direction/forward
start: 58
end: 69
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation1005850
display id: annotation1005850
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005850/range1005850/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_I3425/annotation1005850/range1005850/1
type: Range
persistent identity: range1005850
display id: range1005850
version: 1
top level: BBa_I3425
direction: direction/forward
start: 76
end: 819
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1005858
display id: annotation1005858
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005858/range1005858/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1005858/range1005858/1
type: Range
persistent identity: range1005858
display id: range1005858
version: 1
top level: BBa_I3425
direction: direction/forward
start: 853
end: 864
orientation: inline

BBa_C0083
type: SequenceAnnotation
persistent identity: annotation1005885
display id: annotation1005885
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005885/range1005885/1
component: BBa_C0083

https://synbiohub.org/public/igem/BBa_I3425/annotation1005885/range1005885/1
type: Range
persistent identity: range1005885
display id: range1005885
version: 1
top level: BBa_I3425
direction: direction/forward
start: 871
end: 2388
orientation: inline

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation1005899
display id: annotation1005899
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005899/range1005899/1
component: BBa_R0050

https://synbiohub.org/public/igem/BBa_I3425/annotation1005899/range1005899/1
type: Range
persistent identity: range1005899
display id: range1005899
version: 1
top level: BBa_I3425
direction: direction/forward
start: 2422
end: 2476
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1005909
display id: annotation1005909
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005909/range1005909/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1005909/range1005909/1
type: Range
persistent identity: range1005909
display id: range1005909
version: 1
top level: BBa_I3425
direction: direction/forward
start: 2485
end: 2496
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1005919
display id: annotation1005919
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005919/range1005919/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I3425/annotation1005919/range1005919/1
type: Range
persistent identity: range1005919
display id: range1005919
version: 1
top level: BBa_I3425
direction: direction/forward
start: 2503
end: 3252
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1005925
display id: annotation1005925
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005925/range1005925/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1005925/range1005925/1
type: Range
persistent identity: range1005925
display id: range1005925
version: 1
top level: BBa_I3425
direction: direction/forward
start: 3286
end: 3297
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation1005937
display id: annotation1005937
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005937/range1005937/1
component: BBa_E0032

https://synbiohub.org/public/igem/BBa_I3425/annotation1005937/range1005937/1
type: Range
persistent identity: range1005937
display id: range1005937
version: 1
top level: BBa_I3425
direction: direction/forward
start: 3304
end: 4065
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1005945
display id: annotation1005945
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005945/range1005945/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3425/annotation1005945/range1005945/1
type: Range
persistent identity: range1005945
display id: range1005945
version: 1
top level: BBa_I3425
direction: direction/forward
start: 4074
end: 4153
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1005955
display id: annotation1005955
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005955/range1005955/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3425/annotation1005955/range1005955/1
type: Range
persistent identity: range1005955
display id: range1005955
version: 1
top level: BBa_I3425
direction: direction/forward
start: 4162
end: 4202
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation1005966
display id: annotation1005966
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005966/range1005966/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I3425/annotation1005966/range1005966/1
type: Range
persistent identity: range1005966
display id: range1005966
version: 1
top level: BBa_I3425
direction: direction/forward
start: 4211
end: 4259
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1005971
display id: annotation1005971
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005971/range1005971/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1005971/range1005971/1
type: Range
persistent identity: range1005971
display id: range1005971
version: 1
top level: BBa_I3425
direction: direction/forward
start: 4268
end: 4279
orientation: inline

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation1005981
display id: annotation1005981
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005981/range1005981/1
component: BBa_C0012

https://synbiohub.org/public/igem/BBa_I3425/annotation1005981/range1005981/1
type: Range
persistent identity: range1005981
display id: range1005981
version: 1
top level: BBa_I3425
direction: direction/forward
start: 4286
end: 5413
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1005988
display id: annotation1005988
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005988/range1005988/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3425/annotation1005988/range1005988/1
type: Range
persistent identity: range1005988
display id: range1005988
version: 1
top level: BBa_I3425
direction: direction/forward
start: 5447
end: 5526
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1005998
display id: annotation1005998
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1005998/range1005998/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3425/annotation1005998/range1005998/1
type: Range
persistent identity: range1005998
display id: range1005998
version: 1
top level: BBa_I3425
direction: direction/forward
start: 5535
end: 5575
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1006014
display id: annotation1006014
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006014/range1006014/1
component: BBa_R0010

https://synbiohub.org/public/igem/BBa_I3425/annotation1006014/range1006014/1
type: Range
persistent identity: range1006014
display id: range1006014
version: 1
top level: BBa_I3425
direction: direction/forward
start: 5584
end: 5783
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1006024
display id: annotation1006024
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006024/range1006024/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1006024/range1006024/1
type: Range
persistent identity: range1006024
display id: range1006024
version: 1
top level: BBa_I3425
direction: direction/forward
start: 5792
end: 5803
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation1006039
display id: annotation1006039
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006039/range1006039/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_I3425/annotation1006039/range1006039/1
type: Range
persistent identity: range1006039
display id: range1006039
version: 1
top level: BBa_I3425
direction: direction/forward
start: 5810
end: 6553
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1006047
display id: annotation1006047
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006047/range1006047/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3425/annotation1006047/range1006047/1
type: Range
persistent identity: range1006047
display id: range1006047
version: 1
top level: BBa_I3425
direction: direction/forward
start: 6587
end: 6666
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1006057
display id: annotation1006057
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006057/range1006057/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3425/annotation1006057/range1006057/1
type: Range
persistent identity: range1006057
display id: range1006057
version: 1
top level: BBa_I3425
direction: direction/forward
start: 6675
end: 6715
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation1006068
display id: annotation1006068
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006068/range1006068/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I3425/annotation1006068/range1006068/1
type: Range
persistent identity: range1006068
display id: range1006068
version: 1
top level: BBa_I3425
direction: direction/forward
start: 6724
end: 6772
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1006073
display id: annotation1006073
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006073/range1006073/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1006073/range1006073/1
type: Range
persistent identity: range1006073
display id: range1006073
version: 1
top level: BBa_I3425
direction: direction/forward
start: 6781
end: 6792
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation1006083
display id: annotation1006083
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006083/range1006083/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_I3425/annotation1006083/range1006083/1
type: Range
persistent identity: range1006083
display id: range1006083
version: 1
top level: BBa_I3425
direction: direction/forward
start: 6799
end: 7458
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1006089
display id: annotation1006089
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006089/range1006089/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3425/annotation1006089/range1006089/1
type: Range
persistent identity: range1006089
display id: range1006089
version: 1
top level: BBa_I3425
direction: direction/forward
start: 7492
end: 7571
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1006099
display id: annotation1006099
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006099/range1006099/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3425/annotation1006099/range1006099/1
type: Range
persistent identity: range1006099
display id: range1006099
version: 1
top level: BBa_I3425
direction: direction/forward
start: 7580
end: 7620
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1006114
display id: annotation1006114
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006114/range1006114/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_I3425/annotation1006114/range1006114/1
type: Range
persistent identity: range1006114
display id: range1006114
version: 1
top level: BBa_I3425
direction: direction/forward
start: 7629
end: 7682
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1006122
display id: annotation1006122
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006122/range1006122/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1006122/range1006122/1
type: Range
persistent identity: range1006122
display id: range1006122
version: 1
top level: BBa_I3425
direction: direction/forward
start: 7691
end: 7702
orientation: inline

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation1006137
display id: annotation1006137
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006137/range1006137/1
component: BBa_C0050

https://synbiohub.org/public/igem/BBa_I3425/annotation1006137/range1006137/1
type: Range
persistent identity: range1006137
display id: range1006137
version: 1
top level: BBa_I3425
direction: direction/forward
start: 7709
end: 8452
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1006145
display id: annotation1006145
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006145/range1006145/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3425/annotation1006145/range1006145/1
type: Range
persistent identity: range1006145
display id: range1006145
version: 1
top level: BBa_I3425
direction: direction/forward
start: 8486
end: 8565
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1006155
display id: annotation1006155
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006155/range1006155/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3425/annotation1006155/range1006155/1
type: Range
persistent identity: range1006155
display id: range1006155
version: 1
top level: BBa_I3425
direction: direction/forward
start: 8574
end: 8614
orientation: inline

BBa_R1075
type: SequenceAnnotation
persistent identity: annotation1006166
display id: annotation1006166
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006166/range1006166/1
component: BBa_R1075

https://synbiohub.org/public/igem/BBa_I3425/annotation1006166/range1006166/1
type: Range
persistent identity: range1006166
display id: range1006166
version: 1
top level: BBa_I3425
direction: direction/forward
start: 8623
end: 8671
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1006171
display id: annotation1006171
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006171/range1006171/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1006171/range1006171/1
type: Range
persistent identity: range1006171
display id: range1006171
version: 1
top level: BBa_I3425
direction: direction/forward
start: 8680
end: 8691
orientation: inline

BBa_C0080
type: SequenceAnnotation
persistent identity: annotation1006181
display id: annotation1006181
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006181/range1006181/1
component: BBa_C0080

https://synbiohub.org/public/igem/BBa_I3425/annotation1006181/range1006181/1
type: Range
persistent identity: range1006181
display id: range1006181
version: 1
top level: BBa_I3425
direction: direction/forward
start: 8698
end: 9612
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1006187
display id: annotation1006187
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006187/range1006187/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3425/annotation1006187/range1006187/1
type: Range
persistent identity: range1006187
display id: range1006187
version: 1
top level: BBa_I3425
direction: direction/forward
start: 9646
end: 9725
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1006197
display id: annotation1006197
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006197/range1006197/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3425/annotation1006197/range1006197/1
type: Range
persistent identity: range1006197
display id: range1006197
version: 1
top level: BBa_I3425
direction: direction/forward
start: 9734
end: 9774
orientation: inline

BBa_R0080
type: SequenceAnnotation
persistent identity: annotation1006220
display id: annotation1006220
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006220/range1006220/1
component: BBa_R0080

https://synbiohub.org/public/igem/BBa_I3425/annotation1006220/range1006220/1
type: Range
persistent identity: range1006220
display id: range1006220
version: 1
top level: BBa_I3425
direction: direction/forward
start: 9783
end: 9931
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1006225
display id: annotation1006225
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006225/range1006225/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I3425/annotation1006225/range1006225/1
type: Range
persistent identity: range1006225
display id: range1006225
version: 1
top level: BBa_I3425
direction: direction/forward
start: 9940
end: 9951
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation1006235
display id: annotation1006235
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006235/range1006235/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_I3425/annotation1006235/range1006235/1
type: Range
persistent identity: range1006235
display id: range1006235
version: 1
top level: BBa_I3425
direction: direction/forward
start: 9958
end: 10707
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1006241
display id: annotation1006241
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006241/range1006241/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I3425/annotation1006241/range1006241/1
type: Range
persistent identity: range1006241
display id: range1006241
version: 1
top level: BBa_I3425
direction: direction/forward
start: 10741
end: 10820
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1006251
display id: annotation1006251
version: 1
top level: BBa_I3425
location: https://synbiohub.org/public/igem/BBa_I3425/annotation1006251/range1006251/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I3425/annotation1006251/range1006251/1
type: Range
persistent identity: range1006251
display id: range1006251
version: 1
top level: BBa_I3425
direction: direction/forward
start: 10829
end: 10869
orientation: inline

const.
type: ComponentDefinition
persistent identity: BBa R1075
display id: BBa_R1075
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive Promoter II
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: Gaal, Gourse, et al.
owned by: james
owned by: myers
mutable description: constitutive promoter which outputs .4 TIPs point mutants of wild type rrnB promoter P1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1075_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation309024
display id: annotation309024
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1075/annotation309024/range309024/1
type: Range
persistent identity: range309024
display id: range309024
version: 1
top level: const.
direction: direction/forward
start: 15
end: 20
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation309020
display id: annotation309020
version: 1
top level: const.
location: https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1075/annotation309020/range309020/1
type: Range
persistent identity: range309020
display id: range309020
version: 1
top level: const.
direction: direction/forward
start: 35
end: 40
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa R0010
display id: BBa_R0010
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lacI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
star: true
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: end of LacI coding region (inactive)
sequence annotation: BBa_R0010
sequence annotation: CAP binding site
sequence annotation: -35
sequence annotation: -10
sequence annotation: LacI binding site
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0010_sequence/1

end of LacI coding region (inactive)
type: SequenceAnnotation
persistent identity: annotation1961221
display id: annotation1961221
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_R0010/annotation1961221/range1961221/1
type: Range
persistent identity: range1961221
display id: range1961221
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 88
orientation: inline

BBa_R0010
type: SequenceAnnotation
persistent identity: annotation1961222
display id: annotation1961222
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0010/annotation1961222/range1961222/1
type: Range
persistent identity: range1961222
display id: range1961222
version: 1
top level: LacI
direction: direction/forward
start: 1
end: 200
orientation: inline

CAP binding site
type: SequenceAnnotation
persistent identity: annotation1961223
display id: annotation1961223
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0010/annotation1961223/range1961223/1
type: Range
persistent identity: range1961223
display id: range1961223
version: 1
top level: LacI
direction: direction/forward
start: 89
end: 126
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1961224
display id: annotation1961224
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961224/range1961224/1
type: Range
persistent identity: range1961224
display id: range1961224
version: 1
top level: LacI
direction: direction/forward
start: 137
end: 142
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1961225
display id: annotation1961225
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0010/annotation1961225/range1961225/1
type: Range
persistent identity: range1961225
display id: range1961225
version: 1
top level: LacI
direction: direction/forward
start: 161
end: 166
orientation: inline

LacI binding site
type: SequenceAnnotation
persistent identity: annotation1961226
display id: annotation1961226
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0010/annotation1961226/range1961226/1
type: Range
persistent identity: range1961226
display id: range1961226
version: 1
top level: LacI
direction: direction/forward
start: 166
end: 200
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation1961227
display id: annotation1961227
version: 1
top level: LacI
location: https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0010/annotation1961227/range1961227/1
type: Range
persistent identity: range1961227
display id: range1961227
version: 1
top level: LacI
direction: direction/forward
start: 173
end: 173
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R0051
display id: BBa_R0051
version: 1
was generated by: iGEM to SBOL conversion
description: promoter (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0051_sequence/1

BBa_R0051
type: SequenceAnnotation
persistent identity: annotation7067
display id: annotation7067
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0051/annotation7067/range7067/1
type: Range
persistent identity: range7067
display id: range7067
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2025
display id: annotation2025
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2025/range2025/1
type: Range
persistent identity: range2025
display id: range2025
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2023
display id: annotation2023
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2023/range2023/1
type: Range
persistent identity: range2023
display id: range2023
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2024
display id: annotation2024
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0051/annotation2024/range2024/1
type: Range
persistent identity: range2024
display id: range2024
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2022
display id: annotation2022
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0051/annotation2022/range2022/1
type: Range
persistent identity: range2022
display id: range2022
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

lacI
type: ComponentDefinition
persistent identity: BBa C0012
display id: BBa_C0012
version: 1
was generated by: iGEM to SBOL conversion
description: lacI repressor from E. coli (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription. A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] References (unparsed) here: Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. Nature 403, 335-338 (2000). [ELOW00] Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation. Incompatible with systems containing LacI, lactose, or IPTG.
star: true
creator: Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0012
sequence annotation: lacI-LVA
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0012_sequence/1

BBa_C0012
type: SequenceAnnotation
persistent identity: annotation7031
display id: annotation7031
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_C0012/annotation7031/range7031/1
type: Range
persistent identity: range7031
display id: range7031
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

lacI-LVA
type: SequenceAnnotation
persistent identity: annotation1723
display id: annotation1723
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0012/annotation1723/range1723/1
type: Range
persistent identity: range1723
display id: range1723
version: 1
top level: lacI
direction: direction/forward
start: 1
end: 1128
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation1722
display id: annotation1722
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0012/annotation1722/range1722/1
type: Range
persistent identity: range1722
display id: range1722
version: 1
top level: lacI
direction: direction/forward
start: 1090
end: 1128
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213988
display id: annotation2213988
version: 1
top level: lacI
location: https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0012/annotation2213988/range2213988/1
type: Range
persistent identity: range2213988
display id: range2213988
version: 1
top level: lacI
direction: direction/forward
start: 1129
end: 1153
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa C0050
display id: BBa_C0050
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from phage HK022 (+LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the HK022 bacteriophage cI protein. cI binds to the HK022 pR regulator (BBa_R0050). It represses transcription of the protein encoded by the sequence 3' to the pR region. This coding sequence does not contain a RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. References (unparsed) here: Carlson NG, Little JW. Highly cooperative DNA binding by the coliphage HK022 repressor. J Mol Biol. 1993 Apr 20;230(4):1108-30. PMID: 8487297. Mao C, Little JW. Mutations affecting cooperative DNA binding of phage HK022 CI repressor. J Mol Biol. 1998 May 29;279(1):31-48. PMID: 9636698. Derived from <genbank>STHK022N</genbank>. Response from John Little (Arizona) regarding the start of HK022 cI From: <jlittle@email.arizona.edu> Date: Tue Jan 21, 2003 4:39:21 PM US/Eastern To: "Drew Endy" <endy@MIT.EDU> Subject: RE: hk022 cI start (naive question)? Hello Drew and Michael, I seriously doubt that the extra 27 aa are part of CI. It doesn't make sense in terms of the biology, for sure. In any case, the protein we characterized starts where Carlson and Little stated; as I recall, oR3 partially overlaps the start of cI. I have a vague memory of some possibly interesting biology, having to do with multicopy plasmids. I don't recall the findings themselves. Anyway, one possible explanation was that this extra N-terminal addition was made (e.g. from the pRE promoter, or from a message that arose from transcription around the entire plasmid), making a protein with altered functions. We never followed it up. Good luck John -- Original Message -- Date: Sun, 19 Jan 2003 15:26:26 -0500 Subject: hk022 cI start (naive question)? Cc: elowitm@rockefeller.edu To: jlittle@u.arizona.edu From: Drew Endy <endy@MIT.EDU> Hi John, I'm sitting here with Michael Elowitz and we're working through the sequence for HK022 cI. We noticed that the annotation from NC_002166 includes an "extra" 81 base pairs upstream of what we thought of as the actual start. It look…
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_C0050
sequence annotation: OR3 partial
sequence annotation: cI HK022
sequence annotation: SsrA
sequence annotation: 2
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0050_sequence/1

BBa_C0050
type: SequenceAnnotation
persistent identity: annotation7033
display id: annotation7033
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0050/annotation7033/range7033/1
type: Range
persistent identity: range7033
display id: range7033
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 744
orientation: inline

OR3 partial
type: SequenceAnnotation
persistent identity: annotation1737
display id: annotation1737
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_C0050/annotation1737/range1737/1
type: Range
persistent identity: range1737
display id: range1737
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 8
orientation: inline

cI HK022
type: SequenceAnnotation
persistent identity: annotation1735
display id: annotation1735
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0050/annotation1735/range1735/1
type: Range
persistent identity: range1735
display id: range1735
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 744
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation1736
display id: annotation1736
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0050/annotation1736/range1736/1
type: Range
persistent identity: range1736
display id: range1736
version: 1
top level: cI HK022
direction: direction/forward
start: 706
end: 738
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation1734
display id: annotation1734
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_C0050/annotation1734/range1734/1
type: Range
persistent identity: range1734
display id: range1734
version: 1
top level: cI HK022
direction: direction/forward
start: 739
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213990
display id: annotation2213990
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0050/annotation2213990/range2213990/1
type: Range
persistent identity: range2213990
display id: range2213990
version: 1
top level: cI HK022
direction: direction/forward
start: 745
end: 769
orientation: inline

YFP
type: ComponentDefinition
persistent identity: BBa E0032
display id: BBa_E0032
version: 1
was generated by: iGEM to SBOL conversion
description: enhanced yellow fluorescent protein derived from A. victoria GFP
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: Modified from <bb_part>BBa_E0031</bb_part>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Yellow fluorescent protein (EYFP) reporter coding sequence without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. Annotated mutation cause a Q81L mutation that appears to not be near the active site.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_E0032 yellow fluorescent protein is based on BioBrick part BBa_E0031. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: YFP (LVA)
sequence annotation: BBa_E0032
sequence annotation: A (Q->L)
sequence annotation: SsrA
sequence annotation: 2
sequence: https://synbiohub.org/public/igem/BBa_E0032_sequence/1

YFP (LVA)
type: SequenceAnnotation
persistent identity: annotation2156
display id: annotation2156
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E0032/annotation2156/range2156/1
type: Range
persistent identity: range2156
display id: range2156
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

BBa_E0032
type: SequenceAnnotation
persistent identity: annotation7041
display id: annotation7041
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_E0032/annotation7041/range7041/1
type: Range
persistent identity: range7041
display id: range7041
version: 1
top level: YFP
direction: direction/forward
start: 1
end: 762
orientation: inline

A (Q->L)
type: SequenceAnnotation
persistent identity: annotation2161
display id: annotation2161
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0032/annotation2161/range2161/1
type: Range
persistent identity: range2161
display id: range2161
version: 1
top level: YFP
direction: direction/forward
start: 242
end: 242
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation2160
display id: annotation2160
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_E0032/annotation2160/range2160/1
type: Range
persistent identity: range2160
display id: range2160
version: 1
top level: YFP
direction: direction/forward
start: 718
end: 756
orientation: inline

2
type: SequenceAnnotation
persistent identity: annotation2159
display id: annotation2159
version: 1
top level: YFP
location: https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_E0032/annotation2159/range2159/1
type: Range
persistent identity: range2159
display id: range2159
version: 1
top level: YFP
direction: direction/forward
start: 757
end: 762
orientation: inline

araC
type: ComponentDefinition
persistent identity: BBa C0080
display id: BBa_C0080
version: 1
was generated by: iGEM to SBOL conversion
description: araC arabinose operon regulatory protein (repressor/activator) from E. coli (+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: GenBank: NC_002655 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for the araC gene, used to make araC protein for use in positive or negative regulation of TIPs output (see also R0080, R0081)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: araC
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0080_sequence/1

araC
type: SequenceAnnotation
persistent identity: annotation308191
display id: annotation308191
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0080/annotation308191/range308191/1
type: Range
persistent identity: range308191
display id: range308191
version: 1
top level: araC
direction: direction/forward
start: 1
end: 876
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation308196
display id: annotation308196
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0080/annotation308196/range308196/1
type: Range
persistent identity: range308196
display id: range308196
version: 1
top level: araC
direction: direction/forward
start: 877
end: 909
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214004
display id: annotation2214004
version: 1
top level: araC
location: https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0080/annotation2214004/range2214004/1
type: Range
persistent identity: range2214004
display id: range2214004
version: 1
top level: araC
direction: direction/forward
start: 916
end: 940
orientation: inline

AraC
type: ComponentDefinition
persistent identity: BBa R0080
display id: BBa_R0080
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (AraC regulated)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: GenBank: J01641 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Sara Neves (Fighting Darwins)
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: c-amp2
sequence annotation: araO1
sequence annotation: c-amp1
sequence annotation: ara1 and ara2
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0080_sequence/1

c-amp2
type: SequenceAnnotation
persistent identity: annotation301456
display id: annotation301456
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301456/range301456/1
type: Range
persistent identity: range301456
display id: range301456
version: 1
top level: AraC
direction: direction/forward
start: 4
end: 29
orientation: inline

araO1
type: SequenceAnnotation
persistent identity: annotation301457
display id: annotation301457
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0080/annotation301457/range301457/1
type: Range
persistent identity: range301457
display id: range301457
version: 1
top level: AraC
direction: direction/forward
start: 6
end: 44
orientation: inline

c-amp1
type: SequenceAnnotation
persistent identity: annotation301458
display id: annotation301458
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301458/range301458/1
type: Range
persistent identity: range301458
display id: range301458
version: 1
top level: AraC
direction: direction/forward
start: 43
end: 72
orientation: inline

ara1 and ara2
type: SequenceAnnotation
persistent identity: annotation301462
display id: annotation301462
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0080/annotation301462/range301462/1
type: Range
persistent identity: range301462
display id: range301462
version: 1
top level: AraC
direction: direction/forward
start: 73
end: 101
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation308601
display id: annotation308601
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0080/annotation308601/range308601/1
type: Range
persistent identity: range308601
display id: range308601
version: 1
top level: AraC
direction: direction/forward
start: 113
end: 118
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation308602
display id: annotation308602
version: 1
top level: AraC
location: https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0080/annotation308602/range308602/1
type: Range
persistent identity: range308602
display id: range308602
version: 1
top level: AraC
direction: direction/forward
start: 136
end: 141
orientation: inline

cI HK022
type: ComponentDefinition
persistent identity: BBa R0050
display id: BBa_R0050
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (HK022 cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Bacteriophage HK022.
owned by: james
owned by: myers
mutable description: The pR regulatory region is a 98 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two operator sites at which HK022 cI protein can bind cooperatively. The -35 and -10 regions from pR are included along with some flanking sequences. The cutoff for the 5' end of the regulatory region was placed just after the pM -10 region which promotes transcription of a gene upstream (thus the pM -10 region is not included). The cutoff for the 3' end of the regulatory region was placed immediately prior to the RBS.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Derived from <genbank>STHK022N</genbank>. Literature claims that there is no cross-talk between HK022 cI regulatory region, Lambda cI, 434 cI and P22 cI, but this has not been verified experimentally by the synthetic biology group at MIT.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0050
sequence annotation: -35
sequence annotation: OR2
sequence annotation: -10
sequence annotation: OR1
sequence annotation: putative
sequence: https://synbiohub.org/public/igem/BBa_R0050_sequence/1

BBa_R0050
type: SequenceAnnotation
persistent identity: annotation7066
display id: annotation7066
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0050/annotation7066/range7066/1
type: Range
persistent identity: range7066
display id: range7066
version: 1
top level: cI HK022
direction: direction/forward
start: 1
end: 55
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2012
display id: annotation2012
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0050/annotation2012/range2012/1
type: Range
persistent identity: range2012
display id: range2012
version: 1
top level: cI HK022
direction: direction/forward
start: 8
end: 13
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2013
display id: annotation2013
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_R0050/annotation2013/range2013/1
type: Range
persistent identity: range2013
display id: range2013
version: 1
top level: cI HK022
direction: direction/forward
start: 13
end: 27
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2014
display id: annotation2014
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0050/annotation2014/range2014/1
type: Range
persistent identity: range2014
display id: range2014
version: 1
top level: cI HK022
direction: direction/forward
start: 30
end: 35
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2015
display id: annotation2015
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_R0050/annotation2015/range2015/1
type: Range
persistent identity: range2015
display id: range2015
version: 1
top level: cI HK022
direction: direction/forward
start: 37
end: 51
orientation: inline

putative
type: SequenceAnnotation
persistent identity: annotation2018
display id: annotation2018
version: 1
top level: cI HK022
location: https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_R0050/annotation2018/range2018/1
type: Range
persistent identity: range2018
display id: range2018
version: 1
top level: cI HK022
direction: direction/forward
start: 43
end: 43
orientation: inline

aspA
type: ComponentDefinition
persistent identity: BBa C0083
display id: BBa_C0083
version: 1
was generated by: iGEM to SBOL conversion
description: aspartate ammonia-lyase
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: AE000486 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding sequence for AspA enzyme. AspA aminates fumarate to make aspartate. Aspartate can be used as a bacterial chemotaxis signal.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The start codon was changed from GTG to ATG to match BioBricks standards. Five silent mutations were introduced to eliminate EcoRI and PstI restriction enzyme sites. EcoRI sites were eliminated at bases 588 and 657 with C -> T mutations. PstI sites were eliminated at bases 624, 999, and 1125 with G -> A mutations. The mutations were designed to match codons with similar frequencies of usage in E. coli.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: AspA
sequence annotation: G
sequence annotation: C
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0083_sequence/1

AspA
type: SequenceAnnotation
persistent identity: annotation300981
display id: annotation300981
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0083/annotation300981/range300981/1
type: Range
persistent identity: range300981
display id: range300981
version: 1
top level: aspA
direction: direction/forward
start: 1
end: 1479
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300982
display id: annotation300982
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300982/range300982/1
type: Range
persistent identity: range300982
display id: range300982
version: 1
top level: aspA
direction: direction/forward
start: 1
end: 1
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300983
display id: annotation300983
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300983/range300983/1
type: Range
persistent identity: range300983
display id: range300983
version: 1
top level: aspA
direction: direction/forward
start: 588
end: 588
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300984
display id: annotation300984
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300984/range300984/1
type: Range
persistent identity: range300984
display id: range300984
version: 1
top level: aspA
direction: direction/forward
start: 624
end: 624
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation300986
display id: annotation300986
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300986/range300986/1
type: Range
persistent identity: range300986
display id: range300986
version: 1
top level: aspA
direction: direction/forward
start: 657
end: 657
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300987
display id: annotation300987
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0083/annotation300987/range300987/1
type: Range
persistent identity: range300987
display id: range300987
version: 1
top level: aspA
direction: direction/forward
start: 999
end: 999
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation300988
display id: annotation300988
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_C0083/annotation300988/range300988/1
type: Range
persistent identity: range300988
display id: range300988
version: 1
top level: aspA
direction: direction/forward
start: 1125
end: 1125
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation300993
display id: annotation300993
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0083/annotation300993/range300993/1
type: Range
persistent identity: range300993
display id: range300993
version: 1
top level: aspA
direction: direction/forward
start: 1480
end: 1512
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213998
display id: annotation2213998
version: 1
top level: aspA
location: https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0083/annotation2213998/range2213998/1
type: Range
persistent identity: range2213998
display id: range2213998
version: 1
top level: aspA
direction: direction/forward
start: 1519
end: 1543
orientation: inline

https://synbiohub.org/public/igem/BBa_I3425_sequence/1
type: Sequence
persistent identity: BBa I3425 sequence
display id: BBa_I3425_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagaaagaggagaaatactagatggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccctactagagaaagaggagaaatactagatgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacattcgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattgaaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgcagctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctgaacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacaccaacgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgaccatgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctggtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgtacccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaaaaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccgaaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcgaagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctgtaacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctcttccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgacaccactgttaccatggcagcagaagcaggtcagctgcaattgaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1075_sequence/1
type: Sequence
persistent identity: BBa R1075 sequence
display id: BBa_R1075_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaaatttcctcttttcaggccggaataactccctataatgcgccacca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0010_sequence/1
type: Sequence
persistent identity: BBa R0010 sequence
display id: BBa_R0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0051_sequence/1
type: Sequence
persistent identity: BBa R0051 sequence
display id: BBa_R0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0012_sequence/1
type: Sequence
persistent identity: BBa C0012 sequence
display id: BBa_C0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0050_sequence/1
type: Sequence
persistent identity: BBa C0050 sequence
display id: BBa_C0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggttcaacagaaagagcgtgaaactttctcgcagaggcttgcgctggcctgtgataaagcgggattacctttgcatggtaggcaggctgatttagctgtcaggcttaaggtcacaccaaaagccattagtaaatggttcaacggggagtcaataccaagaaaagacaagatggaatctctggcttcggtgctgggaactactgctgcatatctgcatggctatgctgatgatgacggtatcacggtaaatcatctatcaagatcaaatgattattatcgtgttgatgtattggatgttcaggcgagcgccgggccaggaaccatggtttccaatgaatttatagaaaagataagagcaattgaatatacgaccgagcaggcaagaattttatttaatggaaggccacaggaaagcgtaaaagtcatcacggttcgcggtgacagcatggagggaaccatcaatccgggagatgagatctttgttgatgtatccataacctgttttgatggcgatggcatttatgtgtttgtatacgggaaaacaatgcacgttaagcgcctgcaaatgcaaaagaacaggcttgccgtcatctctgacaatgccgcttatgatcgatggtacatagaagaaggtgaagaagagcaacttcacattctagccaaagtcctcattaggcagtcaatcgattacaagcgattcggagctgcaaacgacgaaaactacgctttagtagcttaataaccctgatagtgctagtgtagatccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0080_sequence/1
type: Sequence
persistent identity: BBa C0080 sequence
display id: BBa_C0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgaagcgcaaaatgatcccctgctgccgggatactcgtttaacgcccatctggtggcgggtttaacgccgattgaggccaatggttatctcgatttttttatcgaccgaccgctgggaatgaaaggttatattctcaatctcaccattcgcggtcagggggtggtgaaaaatcagggacgagaatttgtctgccgaccgggtgatattttgctgttcccgccaggagagattcatcactacggtcgtcatccggaggctcgcgaatggtatcaccagtgggtttactttcgtccgcgcgcctactggcatgaatggcttaactggccgtcaatatttgccaatacgggtttctttcgcccggatgaagcgcaccagccgcatttcagcgacctgtttgggcaaatcattaacgccgggcaaggggaagggcgctattcggagctgctggcgataaatctgcttgagcaattgttactgcggcgcatggaagcgattaacgagtcgctccatccaccgatggataatcgggtacgcgaggcttgtcagtacatcagcgatcacctggcagacagcaattttgatatcgccagcgtcgcacagcatgtttgcctgtcgccgtcgcgtctgtcacatcttttccgccagcagttagggattagcgtcttaagctggcgcgaggaccaacgcatcagccaggcgaagctgcttttgagcactacccggatgcctatcgccaccgtcggtcgcaatgttggttttgacgatcaactctatttctcgcgagtatttaaaaaatgcaccggggccagcccgagcgagttccgtgccggttgtgaagaaaaagtgaatgatgtagccgtcaagttgtcagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0080_sequence/1
type: Sequence
persistent identity: BBa R0080 sequence
display id: BBa_R0080_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0032_sequence/1
type: Sequence
persistent identity: BBa E0032 sequence
display id: BBa_E0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0050_sequence/1
type: Sequence
persistent identity: BBa R0050 sequence
display id: BBa_R0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttaggtattgactgtactatcagttccgtcataatatgaaccataagttcaccac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0083_sequence/1
type: Sequence
persistent identity: BBa C0083 sequence
display id: BBa_C0083_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtgtttaaagcaaatcattggcagcttgaaaaagaaggttcacatgtcaaacaacattcgtatcgaagaagatctgttgggtaccagggaagttccagctgatgcctactatggtgttcacactctgagagcgattgaaaacttctatatcagcaacaacaaaatcagtgatattcctgaatttgttcgcggtatggtaatggttaaaaaagccgcagctatggcaaacaaagagctgcaaaccattcctaaaagtgtagcgaatgccatcattgccgcatgtgatgaagtcctgaacaacggaaaatgcatggatcagttcccggtagacgtctaccagggcggcgcaggtacttccgtaaacatgaacaccaacgaagtgctggccaatatcggtctggaactgatgggtcaccaaaaaggtgaatatcagtacctgaacccgaacgaccatgttaacaaatgtcagtccactaacgacgcctacccgaccggtttccgtatcgcagtttactcttccctgattaagctggtagatgcgattaaccaactgcgtgaaggctttgaacgtaaagctgtcgaatttcaggacatcctgaaaatgggtcgtacccagctgcaagacgcagtaccgatgaccctcggtcaggaatttcgcgctttcagcatcctgctgaaagaagaagtgaaaaacatccaacgtaccgctgaactgctgctggaagttaaccttggtgcaacagcaatcggtactggtctgaacacgccgaaagagtactctccgctggcagtgaaaaaactggctgaagttactggcttcccatgcgtaccggctgaagacctgatcgaagcgacctctgactgcggcgcttatgttatggttcacggcgcgctgaaacgcctggctgtgaagatgtccaaaatctgtaacgacctgcgcttgctctcttcaggcccacgtgccggcctgaacgagatcaacctgccggaactgcaagcgggctcttccatcatgccagctaaagtaaacccggttgttccggaagtggttaaccaggtatgcttcaaagtcatcggtaacgacaccactgttaccatggcagcagaagcaggtcagctgcaattgaacgttatggagccggtcattggccaggccatgttcgaatccgttcacattctgaccaacgcttgctacaacctgctggaaaaatgcattaacggcatcactgctaacaaagaagtgtgcgaaggttacgtttacaactctatcggtatcgttacttacctgaacccgttcatcggtcaccacaacggtgacatcgtgggtaaaatctgtgccgaaaccggtaagagtgtacgtgaagtcgttctggaacgcggtctgttgactgaagcggaacttgacgatattttctccgtacagaatctgatgcacccggcttacaaagcaaaacgctatactgatgaaagcgaacaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z