LOCUS       BBa_I3520               1654 bp    DNA     linear       22-May-2024
DEFINITION  Test: Quorum threshold level (I3510.E0033.B0015)
ACCESSION   BBa_I3520
VERSION     BBa_I3520.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_I3510
     CDS             1165..1512
                     /label=lacZ alpha
     misc_feature    1245..1245
                     /label=T
     misc_feature    1251..1251
                     /label=A
     misc_feature    1272..1272
                     /label=C
     misc_feature    1242..1242
                     /label=C
     misc_feature    1275..1275
                     /label=G
     promoter        1190..1199
                     /label=T3 promoter
     promoter        1337..1355
                     /label=T7 promoter
     misc_feature    1363..1512
                     /label=lacZ gene fragment
     misc_feature    1165..1179
                     /label=lacZ gene fragment
     misc_feature    1165..1517
                     /label=BBa_E0033
     stem_loop       1537..1580
                     /label=stem_loop
     misc_feature    1526..1605
                     /label=BBa_B0010
     terminator      1526..1605
                     /label=BBa_B0010
     stem_loop       1621..1640
                     /label=T7 TE
     misc_feature    1647..1647
                     /label=stop
     polyA_site      1641..1654
                     /label=polya
     misc_feature    1614..1654
                     /label=BBa_B0012
     terminator      1614..1654
                     /label=BBa_B0012
     terminator      1526..1654
                     /label=BBa_B0015
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagatgacc atgattacgc caagcgcgca attaaccctc
     1201 actaaaggga acaaaagctg gagctccacc gcggtggcgg cagcactaga gctagtggat
     1261 cccccgggct gtagaaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     1321 ccggtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     1381 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     1441 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1501 cgcagcctga ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1561 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1621 ggctcacctt cgggtgggcc tttctgcgtt tata
//