LOCUS BBa_I3521 6598 bp DNA linear 22-Nov-2024 DEFINITION Test: Quorum threshold level and chemotaxis response, sender cells (I3600.I3520.I3500) ACCESSION BBa_I3521 VERSION BBa_I3521.1 FEATURES Location/Qualifiers promoter 35..40 /label=-10 promoter 15..20 /label=-35 promoter 1..49 /label=BBa_R1075 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 misc_feature 1165..1203 /label=LVA CDS 76..1203 /label=lacI-LVA misc_feature 76..1203 /label=BBa_C0012 misc_feature 1204..1228 /label=Help:Barcodes CDS 76..1203 /label=BBa_C0012 stem_loop 1248..1291 /label=stem_loop misc_feature 1237..1316 /label=BBa_B0010 terminator 1237..1316 /label=BBa_B0010 stem_loop 1332..1351 /label=T7 TE misc_feature 1358..1358 /label=stop polyA_site 1352..1365 /label=polya misc_feature 1325..1365 /label=BBa_B0012 terminator 1325..1365 /label=BBa_B0012 CDS 1374..1461 /label=end of LacI coding region (inactive) misc_feature 1374..1573 /label=BBa_R0010 misc_feature 1462..1499 /label=CAP binding site promoter 1510..1515 /label=-35 promoter 1534..1539 /label=-10 misc_feature 1539..1573 /label=LacI binding site misc_feature 1546..1546 /label=start promoter 1374..1573 /label=BBa_R0010 misc_feature 1586..1589 /label=conserved RBS 1582..1593 /label=BBa_B0034 CDS 1600..3078 /label=AspA misc_feature 1600..1600 /label=G misc_feature 2187..2187 /label=C misc_feature 2223..2223 /label=G misc_feature 2256..2256 /label=C misc_feature 2598..2598 /label=G misc_feature 2724..2724 /label=G misc_feature 3079..3111 /label=LVA misc_feature 3118..3142 /label=Help:Barcodes CDS 1600..3117 /label=BBa_C0083 misc_feature 3155..3158 /label=conserved RBS 3151..3162 /label=BBa_B0034 CDS 3169..3930 /label=CFP (LVA) misc_feature 3887..3924 /label=SsrA misc_feature 3925..3930 /label=2 misc_feature 3237..3237 /label=A misc_feature 3169..3930 /label=BBa_E0022 misc_feature 3169..3930 /label=BBa_E0022 stem_loop 3950..3993 /label=stem_loop misc_feature 3939..4018 /label=BBa_B0010 terminator 3939..4018 /label=BBa_B0010 stem_loop 4034..4053 /label=T7 TE misc_feature 4060..4060 /label=stop polyA_site 4054..4067 /label=polya misc_feature 4027..4067 /label=BBa_B0012 terminator 4027..4067 /label=BBa_B0012 misc_feature 1..4067 /label=BBa_I3600 misc_feature 4076..4095 /label=LuxR/HSL promoter 4190..4197 /label=-10 promoter 4164..4169 /label=-35 misc_feature 4203..4203 /label=start misc_feature 4205..4224 /label=Putative LuxR/HSL misc_feature 4076..4226 /label=BBa_R0063 promoter 4076..4226 /label=BBa_R0063 misc_feature 4239..4242 /label=conserved RBS 4235..4246 /label=BBa_B0034 misc_feature 4253..4254 /label=prefix misc_feature 4426..4426 /label=T misc_feature 4744..4744 /label=A CDS 4253..5002 /label=luxR misc_feature 4253..5008 /label=BBa_C0062 misc_feature 5009..5033 /label=Help:Barcodes CDS 4253..5008 /label=BBa_C0062 stem_loop 5053..5096 /label=stem_loop misc_feature 5042..5121 /label=BBa_B0010 terminator 5042..5121 /label=BBa_B0010 stem_loop 5137..5156 /label=T7 TE misc_feature 5163..5163 /label=stop polyA_site 5157..5170 /label=polya misc_feature 5130..5170 /label=BBa_B0012 terminator 5130..5170 /label=BBa_B0012 misc_feature 5179..5198 /label=LuxR/HSL promoter 5198..5203 /label=-35 promoter 5220..5225 /label=-10 misc_feature 5231..5231 /label=start misc_feature 5179..5233 /label=BBa_R0062 promoter 5179..5233 /label=BBa_R0062 misc_feature 4076..5233 /label=BBa_I3510 CDS 5240..5587 /label=lacZ alpha misc_feature 5320..5320 /label=T misc_feature 5326..5326 /label=A misc_feature 5347..5347 /label=C misc_feature 5317..5317 /label=C misc_feature 5350..5350 /label=G promoter 5265..5274 /label=T3 promoter promoter 5412..5430 /label=T7 promoter misc_feature 5438..5587 /label=lacZ gene fragment misc_feature 5240..5254 /label=lacZ gene fragment misc_feature 5240..5592 /label=BBa_E0033 stem_loop 5612..5655 /label=stem_loop misc_feature 5601..5680 /label=BBa_B0010 terminator 5601..5680 /label=BBa_B0010 stem_loop 5696..5715 /label=T7 TE misc_feature 5722..5722 /label=stop polyA_site 5716..5729 /label=polya misc_feature 5689..5729 /label=BBa_B0012 terminator 5689..5729 /label=BBa_B0012 terminator 5601..5729 /label=BBa_B0015 misc_feature 4076..5729 /label=BBa_I3520 misc_feature 5738..5757 /label=LuxR/HSL promoter 5757..5762 /label=-35 promoter 5779..5784 /label=-10 misc_feature 5790..5790 /label=start misc_feature 5738..5792 /label=BBa_R0062 promoter 5738..5792 /label=BBa_R0062 misc_feature 5805..5808 /label=conserved RBS 5801..5812 /label=BBa_B0034 misc_feature 6398..6429 /label=LVA CDS 5819..6397 /label=luxI misc_feature 5819..6436 /label=BBa_C0061 misc_feature 6437..6461 /label=Help:Barcodes CDS 5819..6436 /label=BBa_C0061 stem_loop 6481..6524 /label=stem_loop misc_feature 6470..6549 /label=BBa_B0010 terminator 6470..6549 /label=BBa_B0010 stem_loop 6565..6584 /label=T7 TE misc_feature 6591..6591 /label=stop polyA_site 6585..6598 /label=polya misc_feature 6558..6598 /label=BBa_B0012 terminator 6558..6598 /label=BBa_B0012 misc_feature 5738..6598 /label=BBa_I3500 ORIGIN 1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa 61 gaggagaaat actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat 121 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg 181 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg 241 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc 301 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc 361 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac 421 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat 481 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac 541 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag 601 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc 661 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg 721 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg 781 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc 841 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga 901 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat 961 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg 1021 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc 1081 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 1141 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa 1201 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg 1261 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1321 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg 1381 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 1441 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 1501 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 1561 acaatttcac acatactaga gaaagaggag aaatactaga tgtgtttaaa gcaaatcatt 1621 ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga agatctgttg 1681 ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag agcgattgaa 1741 aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg cggtatggta 1801 atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc taaaagtgta 1861 gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg catggatcag 1921 ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa caccaacgaa 1981 gtgctggcca atatcggtct ggaactgatg ggtcaccaaa aaggtgaata tcagtacctg 2041 aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc gaccggtttc 2101 cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca actgcgtgaa 2161 ggctttgaac gtaaagctgt cgaatttcag gacatcctga aaatgggtcg tacccagctg 2221 caagacgcag taccgatgac cctcggtcag gaatttcgcg ctttcagcat cctgctgaaa 2281 gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa ccttggtgca 2341 acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc agtgaaaaaa 2401 ctggctgaag ttactggctt cccatgcgta ccggctgaag acctgatcga agcgacctct 2461 gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt gaagatgtcc 2521 aaaatctgta acgacctgcg cttgctctct tcaggcccac gtgccggcct gaacgagatc 2581 aacctgccgg aactgcaagc gggctcttcc atcatgccag ctaaagtaaa cccggttgtt 2641 ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac tgttaccatg 2701 gcagcagaag caggtcagct gcaattgaac gttatggagc cggtcattgg ccaggccatg 2761 ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa atgcattaac 2821 ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat cggtatcgtt 2881 acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa aatctgtgcc 2941 gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac tgaagcggaa 3001 cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc aaaacgctat 3061 actgatgaaa gcgaacaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 3121 tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat ggtgagcaag 3181 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtgaac 3241 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc 3301 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc 3361 ctgacctggg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc 3421 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac 3481 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc 3541 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac 3601 aactacatca gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc 3661 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag 3721 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc 3781 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc 3841 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagaggcc tgctgcaaac 3901 gacgaaaact acgctttagt agcttaataa tactagagcc aggcatcaaa taaaacgaaa 3961 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4021 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg 4081 tacgatccta caggtgctta tgttaagtaa ttgtattccc agcgatacaa tagtgtgaca 4141 aaaatccaat ttattagaat caaatgtcaa tccattaccg ttttaatgat atataacacg 4201 caaaacttgc gacaaacaat aggtaatact agagaaagag gagaaatact agatgaaaaa 4261 cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa 4321 tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact 4381 cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc 4441 taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga 4501 ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa 4561 taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag 4621 tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa 4681 agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc 4741 ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt 4801 aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat 4861 ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat 4921 gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat 4981 tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga 5041 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5101 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 5161 tgcgtttata tactagagac ctgtaggatc gtacaggttt acgcaagaaa atggtttgtt 5221 atagtcgaat aaatactaga tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 5281 agggaacaaa agctggagct ccaccgcggt ggcggcagca ctagagctag tggatccccc 5341 gggctgtaga aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt 5401 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg 5461 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt 5521 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag 5581 cctgaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 5641 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 5701 accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg tacaggttta 5761 cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga aatactagat 5821 gactataatg ataaaaaaat cggatttttt ggcaattcca tcggaggagt ataaaggtat 5881 tctaagtctt cgttatcaag tgtttaagca aagacttgag tgggacttag ttgtagaaaa 5941 taaccttgaa tcagatgagt atgataactc aaatgcagaa tatatttatg cttgtgatga 6001 tactgaaaat gtaagtggat gctggcgttt attacctaca acaggtgatt atatgctgaa 6061 aagtgttttt cctgaattgc ttggtcaaca gagtgctccc aaagatccta atatagtcga 6121 attaagtcgt tttgctgtag gtaaaaatag ctcaaagata aataactctg ctagtgaaat 6181 tacaatgaaa ctatttgaag ctatatataa acacgctgtt agtcaaggta ttacagaata 6241 tgtaacagta acatcaacag caatagagcg atttttaaag cgtattaaag ttccttgtca 6301 tcgtattgga gacaaagaaa ttcatgtatt aggtgatact aaatcggttg tattgtctat 6361 gcctattaat gaacagttta aaaaagcagt cttaaatgct gcaaacgacg aaaactacgc 6421 tttagtagct taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa 6481 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 6541 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //