LOCUS BBa_I3522 3505 bp DNA linear 22-Nov-2024 DEFINITION Test: Quorum threshold level and chemotaxis response, receiver cells (I3601.I3520.I3500) ACCESSION BBa_I3522 VERSION BBa_I3522.1 FEATURES Location/Qualifiers promoter 35..40 /label=-10 promoter 15..20 /label=-35 promoter 1..49 /label=BBa_R1075 misc_feature 62..65 /label=conserved RBS 58..69 /label=BBa_B0034 CDS 76..837 /label=YFP (LVA) misc_feature 832..837 /label=2 misc_feature 793..831 /label=SsrA misc_feature 317..317 /label=A (Q->L) misc_feature 76..837 /label=BBa_E0032 misc_feature 76..837 /label=BBa_E0032 stem_loop 857..900 /label=stem_loop misc_feature 846..925 /label=BBa_B0010 terminator 846..925 /label=BBa_B0010 stem_loop 941..960 /label=T7 TE misc_feature 967..967 /label=stop polyA_site 961..974 /label=polya misc_feature 934..974 /label=BBa_B0012 terminator 934..974 /label=BBa_B0012 misc_feature 1..974 /label=BBa_I3601 misc_feature 983..1002 /label=LuxR/HSL promoter 1097..1104 /label=-10 promoter 1071..1076 /label=-35 misc_feature 1110..1110 /label=start misc_feature 1112..1131 /label=Putative LuxR/HSL misc_feature 983..1133 /label=BBa_R0063 promoter 983..1133 /label=BBa_R0063 misc_feature 1146..1149 /label=conserved RBS 1142..1153 /label=BBa_B0034 misc_feature 1160..1161 /label=prefix misc_feature 1333..1333 /label=T misc_feature 1651..1651 /label=A CDS 1160..1909 /label=luxR misc_feature 1160..1915 /label=BBa_C0062 misc_feature 1916..1940 /label=Help:Barcodes CDS 1160..1915 /label=BBa_C0062 stem_loop 1960..2003 /label=stem_loop misc_feature 1949..2028 /label=BBa_B0010 terminator 1949..2028 /label=BBa_B0010 stem_loop 2044..2063 /label=T7 TE misc_feature 2070..2070 /label=stop polyA_site 2064..2077 /label=polya misc_feature 2037..2077 /label=BBa_B0012 terminator 2037..2077 /label=BBa_B0012 misc_feature 2086..2105 /label=LuxR/HSL promoter 2105..2110 /label=-35 promoter 2127..2132 /label=-10 misc_feature 2138..2138 /label=start misc_feature 2086..2140 /label=BBa_R0062 promoter 2086..2140 /label=BBa_R0062 misc_feature 983..2140 /label=BBa_I3510 CDS 2147..2494 /label=lacZ alpha misc_feature 2227..2227 /label=T misc_feature 2233..2233 /label=A misc_feature 2254..2254 /label=C misc_feature 2224..2224 /label=C misc_feature 2257..2257 /label=G promoter 2172..2181 /label=T3 promoter promoter 2319..2337 /label=T7 promoter misc_feature 2345..2494 /label=lacZ gene fragment misc_feature 2147..2161 /label=lacZ gene fragment misc_feature 2147..2499 /label=BBa_E0033 stem_loop 2519..2562 /label=stem_loop misc_feature 2508..2587 /label=BBa_B0010 terminator 2508..2587 /label=BBa_B0010 stem_loop 2603..2622 /label=T7 TE misc_feature 2629..2629 /label=stop polyA_site 2623..2636 /label=polya misc_feature 2596..2636 /label=BBa_B0012 terminator 2596..2636 /label=BBa_B0012 terminator 2508..2636 /label=BBa_B0015 misc_feature 983..2636 /label=BBa_I3520 misc_feature 2645..2664 /label=LuxR/HSL promoter 2664..2669 /label=-35 promoter 2686..2691 /label=-10 misc_feature 2697..2697 /label=start misc_feature 2645..2699 /label=BBa_R0062 promoter 2645..2699 /label=BBa_R0062 misc_feature 2712..2715 /label=conserved RBS 2708..2719 /label=BBa_B0034 misc_feature 3305..3336 /label=LVA CDS 2726..3304 /label=luxI misc_feature 2726..3343 /label=BBa_C0061 misc_feature 3344..3368 /label=Help:Barcodes CDS 2726..3343 /label=BBa_C0061 stem_loop 3388..3431 /label=stem_loop misc_feature 3377..3456 /label=BBa_B0010 terminator 3377..3456 /label=BBa_B0010 stem_loop 3472..3491 /label=T7 TE misc_feature 3498..3498 /label=stop polyA_site 3492..3505 /label=polya misc_feature 3465..3505 /label=BBa_B0012 terminator 3465..3505 /label=BBa_B0012 misc_feature 2645..3505 /label=BBa_I3500 ORIGIN 1 ttaaatttcc tcttttcagg ccggaataac tccctataat gcgccaccat actagagaaa 61 gaggagaaat actagatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcaatgctt cgcccgctac 301 cccgaccaca tgaagctgca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag 721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 781 gagctgtaca agaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac 841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 961 tttctgcgtt tatatactag agacctgtac gatcctacag gtgcttatgt taagtaattg 1021 tattcccagc gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc 1081 attaccgttt taatgatata taacacgcaa aacttgcgac aaacaatagg taatactaga 1141 gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa 1201 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa 1261 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc 1321 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa 1381 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg 1441 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa 1501 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg 1561 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc 1621 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat 1681 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc 1741 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt 1801 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat 1861 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg 1921 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 1981 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 2041 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg taggatcgta 2101 caggtttacg caagaaaatg gtttgttata gtcgaataaa tactagatga ccatgattac 2161 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctcca ccgcggtggc 2221 ggcagcacta gagctagtgg atcccccggg ctgtagaaat tcgatatcaa gcttatcgat 2281 accgtcgacc tcgagggggg gcccggtacc caattcgccc tatagtgagt cgtattacgc 2341 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 2401 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 2461 cgatcgccct tcccaacagt tgcgcagcct gaataataat actagagcca ggcatcaaat 2521 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2581 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 2641 agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag tcgaataaat 2701 actagagaaa gaggagaaat actagatgac tataatgata aaaaaatcgg attttttggc 2761 aattccatcg gaggagtata aaggtattct aagtcttcgt tatcaagtgt ttaagcaaag 2821 acttgagtgg gacttagttg tagaaaataa ccttgaatca gatgagtatg ataactcaaa 2881 tgcagaatat atttatgctt gtgatgatac tgaaaatgta agtggatgct ggcgtttatt 2941 acctacaaca ggtgattata tgctgaaaag tgtttttcct gaattgcttg gtcaacagag 3001 tgctcccaaa gatcctaata tagtcgaatt aagtcgtttt gctgtaggta aaaatagctc 3061 aaagataaat aactctgcta gtgaaattac aatgaaacta tttgaagcta tatataaaca 3121 cgctgttagt caaggtatta cagaatatgt aacagtaaca tcaacagcaa tagagcgatt 3181 tttaaagcgt attaaagttc cttgtcatcg tattggagac aaagaaattc atgtattagg 3241 tgatactaaa tcggttgtat tgtctatgcc tattaatgaa cagtttaaaa aagcagtctt 3301 aaatgctgca aacgacgaaa actacgcttt agtagcttaa taactctgat agtgctagtg 3361 tagatctcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 3421 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 3481 tcgggtgggc ctttctgcgt ttata //