LOCUS BBa_I4001 8660 bp DNA linear 16-Dec-2024 DEFINITION Prakash: Design 1 ACCESSION BBa_I4001 VERSION BBa_I4001.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 RBS 106..118 /label=RBS-3\Weak RBS 112..115 /label=RBS misc_feature 106..118 /label=BBa_B0032 RBS 106..118 /label=BBa_B0032 CDS 128..744 /label=tetR misc_feature 745..778 /label=SsrA misc_feature 785..809 /label=Help:Barcodes CDS 125..784 /label=BBa_C0040 stem_loop 829..872 /label=stem_loop misc_feature 818..897 /label=BBa_B0010 terminator 818..897 /label=BBa_B0010 stem_loop 913..932 /label=T7 TE misc_feature 939..939 /label=stop polyA_site 933..946 /label=polya misc_feature 906..946 /label=BBa_B0012 terminator 906..946 /label=BBa_B0012 misc_feature 955..973 /label=TetR 1 misc_feature 955..1008 /label=BBa_R0040 promoter 974..979 /label=-35 misc_feature 980..998 /label=TetR 2 promoter 997..1002 /label=-10 promoter 955..1008 /label=BBa_R0040 RBS 1017..1029 /label=RBS-3\Weak RBS 1023..1026 /label=RBS misc_feature 1017..1029 /label=BBa_B0032 RBS 1017..1029 /label=BBa_B0032 misc_feature 2125..2163 /label=LVA CDS 1036..2163 /label=lacI-LVA misc_feature 1036..2163 /label=BBa_C0012 misc_feature 2164..2188 /label=Help:Barcodes CDS 1036..2163 /label=BBa_C0012 stem_loop 2208..2251 /label=stem_loop misc_feature 2197..2276 /label=BBa_B0010 terminator 2197..2276 /label=BBa_B0010 stem_loop 2292..2311 /label=T7 TE misc_feature 2318..2318 /label=stop polyA_site 2312..2325 /label=polya misc_feature 2285..2325 /label=BBa_B0012 terminator 2285..2325 /label=BBa_B0012 CDS 2334..2421 /label=end of LacI coding region (inactive) misc_feature 2334..2533 /label=BBa_R0010 misc_feature 2422..2459 /label=CAP binding site promoter 2470..2475 /label=-35 promoter 2494..2499 /label=-10 misc_feature 2499..2533 /label=LacI binding site misc_feature 2506..2506 /label=start promoter 2334..2533 /label=BBa_R0010 RBS 2542..2554 /label=RBS-3\Weak RBS 2548..2551 /label=RBS misc_feature 2542..2554 /label=BBa_B0032 RBS 2542..2554 /label=BBa_B0032 CDS 2564..3271 /label=cI lambda misc_feature 3272..3304 /label=LVA misc_feature 3311..3335 /label=Help:Barcodes CDS 2561..3310 /label=BBa_C0051 stem_loop 3355..3398 /label=stem_loop misc_feature 3344..3423 /label=BBa_B0010 terminator 3344..3423 /label=BBa_B0010 stem_loop 3439..3458 /label=T7 TE misc_feature 3465..3465 /label=stop polyA_site 3459..3472 /label=polya misc_feature 3432..3472 /label=BBa_B0012 terminator 3432..3472 /label=BBa_B0012 misc_feature 3481..3500 /label=LuxR/HSL promoter 3595..3602 /label=-10 promoter 3569..3574 /label=-35 misc_feature 3608..3608 /label=start misc_feature 3610..3629 /label=Putative LuxR/HSL misc_feature 3481..3631 /label=BBa_R0063 promoter 3481..3631 /label=BBa_R0063 RBS 3640..3654 /label=RBS-1\Strong RBS 3647..3651 /label=RBS misc_feature 3640..3654 /label=BBa_B0030 RBS 3640..3654 /label=BBa_B0030 misc_feature 3661..3662 /label=prefix misc_feature 3834..3834 /label=T misc_feature 4152..4152 /label=A CDS 3661..4410 /label=luxR misc_feature 3661..4416 /label=BBa_C0062 misc_feature 4417..4441 /label=Help:Barcodes CDS 3661..4416 /label=BBa_C0062 stem_loop 4461..4504 /label=stem_loop misc_feature 4450..4529 /label=BBa_B0010 terminator 4450..4529 /label=BBa_B0010 stem_loop 4545..4564 /label=T7 TE misc_feature 4571..4571 /label=stop polyA_site 4565..4578 /label=polya misc_feature 4538..4578 /label=BBa_B0012 terminator 4538..4578 /label=BBa_B0012 misc_feature 4587..4683 /label=BBa_R0065 misc_feature 4592..4611 /label=Lux Box promoter 4633..4638 /label=-10 misc_feature 4643..4659 /label=OR2 cI promoter 4644..4644 /label=lux p(R) start promoter 4657..4662 /label=-35 misc_feature 4667..4683 /label=OR1 cI promoter 4680..4683 /label=-10 promoter 4587..4683 /label=BBa_R0065 RBS 4692..4706 /label=RBS-1\Strong RBS 4699..4703 /label=RBS misc_feature 4692..4706 /label=BBa_B0030 RBS 4692..4706 /label=BBa_B0030 misc_feature 5292..5323 /label=LVA CDS 4713..5291 /label=luxI misc_feature 4713..5330 /label=BBa_C0061 misc_feature 5331..5355 /label=Help:Barcodes CDS 4713..5330 /label=BBa_C0061 stem_loop 5375..5418 /label=stem_loop misc_feature 5364..5443 /label=BBa_B0010 terminator 5364..5443 /label=BBa_B0010 stem_loop 5459..5478 /label=T7 TE misc_feature 5485..5485 /label=stop polyA_site 5479..5492 /label=polya misc_feature 5452..5492 /label=BBa_B0012 terminator 5452..5492 /label=BBa_B0012 CDS 5501..5588 /label=end of LacI coding region (inactive) misc_feature 5501..5700 /label=BBa_R0010 misc_feature 5589..5626 /label=CAP binding site promoter 5637..5642 /label=-35 promoter 5661..5666 /label=-10 misc_feature 5666..5700 /label=LacI binding site misc_feature 5673..5673 /label=start promoter 5501..5700 /label=BBa_R0010 RBS 5709..5723 /label=RBS-1\Strong RBS 5716..5720 /label=RBS misc_feature 5709..5723 /label=BBa_B0030 RBS 5709..5723 /label=BBa_B0030 misc_feature 5730..5732 /label=start misc_feature 6513..6518 /label=2 misc_feature 6480..6512 /label=LVA CDS 5730..6479 /label=aiiA misc_feature 5730..6518 /label=BBa_C0060 misc_feature 6519..6543 /label=Help:Barcodes CDS 5730..6518 /label=BBa_C0060 stem_loop 6563..6606 /label=stem_loop misc_feature 6552..6631 /label=BBa_B0010 terminator 6552..6631 /label=BBa_B0010 stem_loop 6647..6666 /label=T7 TE misc_feature 6673..6673 /label=stop polyA_site 6667..6680 /label=polya misc_feature 6640..6680 /label=BBa_B0012 terminator 6640..6680 /label=BBa_B0012 misc_feature 6689..6707 /label=TetR 1 misc_feature 6689..6742 /label=BBa_R0040 promoter 6708..6713 /label=-35 misc_feature 6714..6732 /label=TetR 2 promoter 6731..6736 /label=-10 promoter 6689..6742 /label=BBa_R0040 RBS 6751..6765 /label=RBS-1\Strong RBS 6758..6762 /label=RBS misc_feature 6751..6765 /label=BBa_B0030 RBS 6751..6765 /label=BBa_B0030 CDS 6772..7533 /label=CFP (LVA) misc_feature 7490..7527 /label=SsrA misc_feature 7528..7533 /label=2 misc_feature 6840..6840 /label=A misc_feature 6772..7533 /label=BBa_E0022 misc_feature 6772..7533 /label=BBa_E0022 stem_loop 7553..7596 /label=stem_loop misc_feature 7542..7621 /label=BBa_B0010 terminator 7542..7621 /label=BBa_B0010 stem_loop 7637..7656 /label=T7 TE misc_feature 7663..7663 /label=stop polyA_site 7657..7670 /label=polya misc_feature 7630..7670 /label=BBa_B0012 terminator 7630..7670 /label=BBa_B0012 misc_feature 7679..7697 /label=TetR 1 misc_feature 7679..7732 /label=BBa_R0040 promoter 7698..7703 /label=-35 misc_feature 7704..7722 /label=TetR 2 promoter 7721..7726 /label=-10 promoter 7679..7732 /label=BBa_R0040 RBS 7741..7755 /label=RBS-1\Strong RBS 7748..7752 /label=RBS misc_feature 7741..7755 /label=BBa_B0030 RBS 7741..7755 /label=BBa_B0030 CDS 7762..8523 /label=YFP (LVA) misc_feature 8518..8523 /label=2 misc_feature 8479..8517 /label=SsrA misc_feature 8003..8003 /label=A (Q->L) misc_feature 7762..8523 /label=BBa_E0032 misc_feature 7762..8523 /label=BBa_E0032 stem_loop 8543..8586 /label=stem_loop misc_feature 8532..8611 /label=BBa_B0010 terminator 8532..8611 /label=BBa_B0010 stem_loop 8627..8646 /label=T7 TE misc_feature 8653..8653 /label=stop polyA_site 8647..8660 /label=polya misc_feature 8620..8660 /label=BBa_B0012 terminator 8620..8660 /label=BBa_B0012 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagtcaca caggaaagta 121 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga 181 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc 241 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat 301 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt 361 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt 421 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt 481 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca 541 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga 601 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca 661 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa 721 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta 781 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag 841 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 901 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta 961 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagtcac 1021 acaggaaagt actagatggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat 1081 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg 1141 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg 1201 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc 1261 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc 1321 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac 1381 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat 1441 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac 1501 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag 1561 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc 1621 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg 1681 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg 1741 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc 1801 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga 1861 tacgacgata ccgaagacag ctcatgttat atcccgccgt taaccaccat caaacaggat 1921 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg 1981 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc 2041 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 2101 gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa actacgcttt agtagcttaa 2161 taactctgat agtgctagtg tagatctcta ctagagccag gcatcaaata aaacgaaagg 2221 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 2281 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagcaatacg 2341 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 2401 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 2461 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 2521 acaatttcac acatactaga gtcacacagg aaagtactag atgagcacaa aaaagaaacc 2581 attaacacaa gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa 2641 aaatgaactt ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg 2701 cgttggtgct ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc 2761 aaaaattctc aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga 2821 gatgtatgaa gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt 2881 ttctcatgtt caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc 2941 ggagagatgg gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga 3001 aggtaattcc atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat 3061 tctcgttgac cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg 3121 tgatgagttt accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact 3181 aaacccacag tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat 3241 cgctagtcag tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt 3301 agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa 3361 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 3421 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag 3481 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 3541 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 3601 acacgcaaaa cttgcgacaa acaataggta atactagaga ttaaagagga gaaatactag 3661 atgaaaaaca taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga 3721 agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat 3781 tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat 3841 aattacccta aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct 3901 atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat 3961 gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact 4021 gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat 4081 tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta 4141 attgttcctt ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac 4201 aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct 4261 tgggatattt caaaaatatt aggttgcagt gagcgtactg tcactttcca tttaaccaat 4321 gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca 4381 ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca 4441 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 4501 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 4561 ggcctttctg cgtttatata ctagagtaag cacctgtagg atcgtacagg tttacgcaag 4621 aaaatggttt gttatagtcg aataacaccg tgcgtgttga ctattttacc tctggcggtg 4681 atatactaga gattaaagag gagaaatact agatgactat aatgataaaa aaatcggatt 4741 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta 4801 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata 4861 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc 4921 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc 4981 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa 5041 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat 5101 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag 5161 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg 5221 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag 5281 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt 5341 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 5401 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 5461 ctcaccttcg ggtgggcctt tctgcgttta tatactagag caatacgcaa accgcctctc 5521 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 5581 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta 5641 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca 5701 tactagagat taaagaggag aaatactaga tgacagtaaa gaagctttat ttcgtcccag 5761 caggtcgttg tatgttggat cattcgtctg ttaatagtac attaacacca ggagaattat 5821 tagacttacc ggtttggtgt tatcttttgg agactgaaga aggacctatt ttagtagata 5881 caggtatgcc agaaagtgca gttaataatg aaggtctttt taacggtaca tttgtcgaag 5941 ggcaggtttt accgaaaatg actgaagaag atagaatcgt gaatatttta aaacgggttg 6001 gttatgagcc ggaagacctt ctttatatta ttagttctca cttgcatttt gatcatgcag 6061 gaggaaatgg cgcttttata aatacaccaa tcattgtaca gcgtgctgaa tatgaggcgg 6121 cgcagcatag cgaagaatat ttgaaagaat gtatattgcc gaatttaaac tacaaaatca 6181 ttgaaggtga ttatgaagtc gtaccaggag ttcaattatt gcatacacca ggccatactc 6241 cagggcatca atcgctatta attgagacag aaaaatccgg tcctgtatta ttaacgattg 6301 atgcatcgta tacgaaagag aattttgaaa atgaagtgcc atttgcggga tttgattcag 6361 aattagcttt atcttcaatt aaacgtttaa aagaagtggt gatgaaagag aagccgattg 6421 ttttctttgg acatgatata gagcaggaaa ggggatgtaa agtgttccct gaatatatag 6481 ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat 6541 cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 6601 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 6661 tgggcctttc tgcgtttata tactagagtc cctatcagtg atagagattg acatccctat 6721 cagtgataga gatactgagc actactagag attaaagagg agaaatacta gatggtgagc 6781 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgtg 6841 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 6901 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 6961 accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 7021 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 7081 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 7141 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 7201 tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag 7261 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 7321 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 7381 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 7441 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagag gcctgctgca 7501 aacgacgaaa actacgcttt agtagcttaa taatactaga gccaggcatc aaataaaacg 7561 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 7621 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc 7681 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag 7741 attaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg 7801 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag 7861 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag 7921 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc 7981 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac 8041 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg 8101 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag 8161 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc 8221 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag 8281 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc 8341 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac 8401 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc 8461 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa 8521 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 8581 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 8641 tgggcctttc tgcgtttata //