LOCUS BBa_I4003 8612 bp DNA linear 16-Dec-2024 DEFINITION Prakash: Design 3 ACCESSION BBa_I4003 VERSION BBa_I4003.1 FEATURES Location/Qualifiers promoter 38..43 /label=-10 promoter 15..20 /label=-35 misc_feature 25..41 /label=OR1 misc_feature 1..17 /label=OR2 misc_feature 1..49 /label=BBa_R0051 promoter 1..49 /label=BBa_R0051 RBS 58..70 /label=RBS-3\Weak RBS 64..67 /label=RBS misc_feature 58..70 /label=BBa_B0032 RBS 58..70 /label=BBa_B0032 CDS 80..696 /label=tetR misc_feature 697..730 /label=SsrA misc_feature 737..761 /label=Help:Barcodes CDS 77..736 /label=BBa_C0040 stem_loop 781..824 /label=stem_loop misc_feature 770..849 /label=BBa_B0010 terminator 770..849 /label=BBa_B0010 stem_loop 865..884 /label=T7 TE misc_feature 891..891 /label=stop polyA_site 885..898 /label=polya misc_feature 858..898 /label=BBa_B0012 terminator 858..898 /label=BBa_B0012 misc_feature 907..925 /label=TetR 1 misc_feature 907..960 /label=BBa_R0040 promoter 926..931 /label=-35 misc_feature 932..950 /label=TetR 2 promoter 949..954 /label=-10 promoter 907..960 /label=BBa_R0040 RBS 969..981 /label=RBS-3\Weak RBS 975..978 /label=RBS misc_feature 969..981 /label=BBa_B0032 RBS 969..981 /label=BBa_B0032 misc_feature 2077..2115 /label=LVA CDS 988..2115 /label=lacI-LVA misc_feature 988..2115 /label=BBa_C0012 misc_feature 2116..2140 /label=Help:Barcodes CDS 988..2115 /label=BBa_C0012 stem_loop 2160..2203 /label=stem_loop misc_feature 2149..2228 /label=BBa_B0010 terminator 2149..2228 /label=BBa_B0010 stem_loop 2244..2263 /label=T7 TE misc_feature 2270..2270 /label=stop polyA_site 2264..2277 /label=polya misc_feature 2237..2277 /label=BBa_B0012 terminator 2237..2277 /label=BBa_B0012 CDS 2286..2373 /label=end of LacI coding region (inactive) misc_feature 2286..2485 /label=BBa_R0010 misc_feature 2374..2411 /label=CAP binding site promoter 2422..2427 /label=-35 promoter 2446..2451 /label=-10 misc_feature 2451..2485 /label=LacI binding site misc_feature 2458..2458 /label=start promoter 2286..2485 /label=BBa_R0010 RBS 2494..2506 /label=RBS-3\Weak RBS 2500..2503 /label=RBS misc_feature 2494..2506 /label=BBa_B0032 RBS 2494..2506 /label=BBa_B0032 CDS 2516..3223 /label=cI lambda misc_feature 3224..3256 /label=LVA misc_feature 3263..3287 /label=Help:Barcodes CDS 2513..3262 /label=BBa_C0051 stem_loop 3307..3350 /label=stem_loop misc_feature 3296..3375 /label=BBa_B0010 terminator 3296..3375 /label=BBa_B0010 stem_loop 3391..3410 /label=T7 TE misc_feature 3417..3417 /label=stop polyA_site 3411..3424 /label=polya misc_feature 3384..3424 /label=BBa_B0012 terminator 3384..3424 /label=BBa_B0012 misc_feature 3433..3452 /label=LuxR/HSL promoter 3547..3554 /label=-10 promoter 3521..3526 /label=-35 misc_feature 3560..3560 /label=start misc_feature 3562..3581 /label=Putative LuxR/HSL misc_feature 3433..3583 /label=BBa_R0063 promoter 3433..3583 /label=BBa_R0063 RBS 3592..3606 /label=RBS-1\Strong RBS 3599..3603 /label=RBS misc_feature 3592..3606 /label=BBa_B0030 RBS 3592..3606 /label=BBa_B0030 misc_feature 3613..3614 /label=prefix misc_feature 3786..3786 /label=T misc_feature 4104..4104 /label=A CDS 3613..4362 /label=luxR misc_feature 3613..4368 /label=BBa_C0062 misc_feature 4369..4393 /label=Help:Barcodes CDS 3613..4368 /label=BBa_C0062 stem_loop 4413..4456 /label=stem_loop misc_feature 4402..4481 /label=BBa_B0010 terminator 4402..4481 /label=BBa_B0010 stem_loop 4497..4516 /label=T7 TE misc_feature 4523..4523 /label=stop polyA_site 4517..4530 /label=polya misc_feature 4490..4530 /label=BBa_B0012 terminator 4490..4530 /label=BBa_B0012 misc_feature 4539..4635 /label=BBa_R0065 misc_feature 4544..4563 /label=Lux Box promoter 4585..4590 /label=-10 misc_feature 4595..4611 /label=OR2 cI promoter 4596..4596 /label=lux p(R) start promoter 4609..4614 /label=-35 misc_feature 4619..4635 /label=OR1 cI promoter 4632..4635 /label=-10 promoter 4539..4635 /label=BBa_R0065 RBS 4644..4658 /label=RBS-1\Strong RBS 4651..4655 /label=RBS misc_feature 4644..4658 /label=BBa_B0030 RBS 4644..4658 /label=BBa_B0030 misc_feature 5244..5275 /label=LVA CDS 4665..5243 /label=luxI misc_feature 4665..5282 /label=BBa_C0061 misc_feature 5283..5307 /label=Help:Barcodes CDS 4665..5282 /label=BBa_C0061 stem_loop 5327..5370 /label=stem_loop misc_feature 5316..5395 /label=BBa_B0010 terminator 5316..5395 /label=BBa_B0010 stem_loop 5411..5430 /label=T7 TE misc_feature 5437..5437 /label=stop polyA_site 5431..5444 /label=polya misc_feature 5404..5444 /label=BBa_B0012 terminator 5404..5444 /label=BBa_B0012 CDS 5453..5540 /label=end of LacI coding region (inactive) misc_feature 5453..5652 /label=BBa_R0010 misc_feature 5541..5578 /label=CAP binding site promoter 5589..5594 /label=-35 promoter 5613..5618 /label=-10 misc_feature 5618..5652 /label=LacI binding site misc_feature 5625..5625 /label=start promoter 5453..5652 /label=BBa_R0010 RBS 5661..5675 /label=RBS-1\Strong RBS 5668..5672 /label=RBS misc_feature 5661..5675 /label=BBa_B0030 RBS 5661..5675 /label=BBa_B0030 misc_feature 5682..5684 /label=start misc_feature 6465..6470 /label=2 misc_feature 6432..6464 /label=LVA CDS 5682..6431 /label=aiiA misc_feature 5682..6470 /label=BBa_C0060 misc_feature 6471..6495 /label=Help:Barcodes CDS 5682..6470 /label=BBa_C0060 stem_loop 6515..6558 /label=stem_loop misc_feature 6504..6583 /label=BBa_B0010 terminator 6504..6583 /label=BBa_B0010 stem_loop 6599..6618 /label=T7 TE misc_feature 6625..6625 /label=stop polyA_site 6619..6632 /label=polya misc_feature 6592..6632 /label=BBa_B0012 terminator 6592..6632 /label=BBa_B0012 misc_feature 6641..6659 /label=TetR 1 misc_feature 6641..6694 /label=BBa_R0040 promoter 6660..6665 /label=-35 misc_feature 6666..6684 /label=TetR 2 promoter 6683..6688 /label=-10 promoter 6641..6694 /label=BBa_R0040 RBS 6703..6717 /label=RBS-1\Strong RBS 6710..6714 /label=RBS misc_feature 6703..6717 /label=BBa_B0030 RBS 6703..6717 /label=BBa_B0030 CDS 6724..7485 /label=CFP (LVA) misc_feature 7442..7479 /label=SsrA misc_feature 7480..7485 /label=2 misc_feature 6792..6792 /label=A misc_feature 6724..7485 /label=BBa_E0022 misc_feature 6724..7485 /label=BBa_E0022 stem_loop 7505..7548 /label=stem_loop misc_feature 7494..7573 /label=BBa_B0010 terminator 7494..7573 /label=BBa_B0010 stem_loop 7589..7608 /label=T7 TE misc_feature 7615..7615 /label=stop polyA_site 7609..7622 /label=polya misc_feature 7582..7622 /label=BBa_B0012 terminator 7582..7622 /label=BBa_B0012 misc_feature 7631..7649 /label=TetR 1 misc_feature 7631..7684 /label=BBa_R0040 promoter 7650..7655 /label=-35 misc_feature 7656..7674 /label=TetR 2 promoter 7673..7678 /label=-10 promoter 7631..7684 /label=BBa_R0040 RBS 7693..7707 /label=RBS-1\Strong RBS 7700..7704 /label=RBS misc_feature 7693..7707 /label=BBa_B0030 RBS 7693..7707 /label=BBa_B0030 CDS 7714..8475 /label=YFP (LVA) misc_feature 8470..8475 /label=2 misc_feature 8431..8469 /label=SsrA misc_feature 7955..7955 /label=A (Q->L) misc_feature 7714..8475 /label=BBa_E0032 misc_feature 7714..8475 /label=BBa_E0032 stem_loop 8495..8538 /label=stem_loop misc_feature 8484..8563 /label=BBa_B0010 terminator 8484..8563 /label=BBa_B0010 stem_loop 8579..8598 /label=T7 TE misc_feature 8605..8605 /label=stop polyA_site 8599..8612 /label=polya misc_feature 8572..8612 /label=BBa_B0012 terminator 8572..8612 /label=BBa_B0012 ORIGIN 1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagtca 61 cacaggaaag tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga 121 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg 181 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt 241 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg 301 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga 361 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa 421 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag 481 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa 541 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga 601 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg 661 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc 721 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 781 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 841 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 901 ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac 961 tactagagtc acacaggaaa gtactagatg gtgaatgtga aaccagtaac gttatacgat 1021 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 1081 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 1141 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 1201 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 1261 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 1321 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 1381 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 1441 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 1501 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 1561 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 1621 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 1681 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 1741 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 1801 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 1861 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 1921 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 1981 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 2041 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct 2101 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa 2161 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 2221 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 2281 tagagcaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 2341 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 2401 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 2461 tgtgagcgga taacaatttc acacatacta gagtcacaca ggaaagtact agatgagcac 2521 aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta 2581 tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat 2641 ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc 2701 cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag 2761 agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga 2821 gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac 2881 caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg 2941 gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga 3001 cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc 3061 cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt 3121 tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt 3181 ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa 3241 ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 3301 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 3361 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 3421 tatatactag agacctgtac gatcctacag gtgcttatgt taagtaattg tattcccagc 3481 gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc attaccgttt 3541 taatgatata taacacgcaa aacttgcgac aaacaatagg taatactaga gattaaagag 3601 gagaaatact agatgaaaaa cataaatgcc gacgacacat acagaataat taataaaatt 3661 aaagcttgta gaagcaataa tgatattaat caatgcttat ctgatatgac taaaatggta 3721 cattgtgaat attatttact cgcgatcatt tatcctcatt ctatggttaa atctgatatt 3781 tcaatcctag ataattaccc taaaaaatgg aggcaatatt atgatgacgc taatttaata 3841 aaatatgatc ctatagtaga ttattctaac tccaatcatt caccaattaa ttggaatata 3901 tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca 3961 ggtcttatca ctgggtttag tttccctatt catacggcta acaatggctt cggaatgctt 4021 agttttgcac attcagaaaa agacaactat atagatagtt tatttttaca tgcgtgtatg 4081 aacataccat taattgttcc ttctctagtt gataattatc gaaaaataaa tatagcaaat 4141 aataaatcaa acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa 4201 ggaaaaagct cttgggatat ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc 4261 catttaacca atgcgcaaat gaaactcaat acaacaaacc gctgccaaag tatttctaaa 4321 gcaattttaa caggagcaat tgattgccca tactttaaaa attaataaca ctgatagtgc 4381 tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 4441 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 4501 caccttcggg tgggcctttc tgcgtttata tactagagta agcacctgta ggatcgtaca 4561 ggtttacgca agaaaatggt ttgttatagt cgaataacac cgtgcgtgtt gactatttta 4621 cctctggcgg tgatatacta gagattaaag aggagaaata ctagatgact ataatgataa 4681 aaaaatcgga ttttttggca attccatcgg aggagtataa aggtattcta agtcttcgtt 4741 atcaagtgtt taagcaaaga cttgagtggg acttagttgt agaaaataac cttgaatcag 4801 atgagtatga taactcaaat gcagaatata tttatgcttg tgatgatact gaaaatgtaa 4861 gtggatgctg gcgtttatta cctacaacag gtgattatat gctgaaaagt gtttttcctg 4921 aattgcttgg tcaacagagt gctcccaaag atcctaatat agtcgaatta agtcgttttg 4981 ctgtaggtaa aaatagctca aagataaata actctgctag tgaaattaca atgaaactat 5041 ttgaagctat atataaacac gctgttagtc aaggtattac agaatatgta acagtaacat 5101 caacagcaat agagcgattt ttaaagcgta ttaaagttcc ttgtcatcgt attggagaca 5161 aagaaattca tgtattaggt gatactaaat cggttgtatt gtctatgcct attaatgaac 5221 agtttaaaaa agcagtctta aatgctgcaa acgacgaaaa ctacgcttta gtagcttaat 5281 aactctgata gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc 5341 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 5401 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agcaatacgc 5461 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc 5521 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca 5581 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa 5641 caatttcaca catactagag attaaagagg agaaatacta gatgacagta aagaagcttt 5701 atttcgtccc agcaggtcgt tgtatgttgg atcattcgtc tgttaatagt acattaacac 5761 caggagaatt attagactta ccggtttggt gttatctttt ggagactgaa gaaggaccta 5821 ttttagtaga tacaggtatg ccagaaagtg cagttaataa tgaaggtctt tttaacggta 5881 catttgtcga agggcaggtt ttaccgaaaa tgactgaaga agatagaatc gtgaatattt 5941 taaaacgggt tggttatgag ccggaagacc ttctttatat tattagttct cacttgcatt 6001 ttgatcatgc aggaggaaat ggcgctttta taaatacacc aatcattgta cagcgtgctg 6061 aatatgaggc ggcgcagcat agcgaagaat atttgaaaga atgtatattg ccgaatttaa 6121 actacaaaat cattgaaggt gattatgaag tcgtaccagg agttcaatta ttgcatacac 6181 caggccatac tccagggcat caatcgctat taattgagac agaaaaatcc ggtcctgtat 6241 tattaacgat tgatgcatcg tatacgaaag agaattttga aaatgaagtg ccatttgcgg 6301 gatttgattc agaattagct ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag 6361 agaagccgat tgttttcttt ggacatgata tagagcagga aaggggatgt aaagtgttcc 6421 ctgaatatat agctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 6481 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 6541 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 6601 ctcaccttcg ggtgggcctt tctgcgttta tatactagag tccctatcag tgatagagat 6661 tgacatccct atcagtgata gagatactga gcactactag agattaaaga ggagaaatac 6721 tagatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg 6781 gacggcgacg tgaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc 6841 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc 6901 accctcgtga ccaccctgac ctggggcgtg cagtgcttca gccgctaccc cgaccacatg 6961 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc 7021 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc 7081 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg 7141 cacaagctgg agtacaacta catcagccac aacgtctata tcaccgccga caagcagaag 7201 aacggcatca aggccaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc 7261 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac 7321 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg 7381 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag 7441 aggcctgctg caaacgacga aaactacgct ttagtagctt aataatacta gagccaggca 7501 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc 7561 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 7621 tatactagag tccctatcag tgatagagat tgacatccct atcagtgata gagatactga 7681 gcactactag agattaaaga ggagaaatac tagatggtga gcaagggcga ggagctgttc 7741 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 7801 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 7861 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg 7921 caatgcttcg cccgctaccc cgaccacatg aagctgcacg acttcttcaa gtccgccatg 7981 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 8041 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 8101 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 8161 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 8221 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 8281 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc 8341 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 8401 atcactctcg gcatggacga gctgtacaag aggcctgctg caaacgacga aaactacgct 8461 ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 8521 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 8581 ctcaccttcg ggtgggcctt tctgcgttta ta //