##gff-version 3
##sequence-region BBa_I4101 1 4473
BBa_I4101	.	ribosome_entry_site	2437	2449	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I4101	.	ribosome_entry_site	2443	2446	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I4101	.	ribosome_entry_site	2437	2449	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I4101	.	engineered_region	2437	2449	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I4101	.	ribosome_entry_site	3535	3549	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4101	.	engineered_region	3535	3549	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4101	.	ribosome_entry_site	3542	3546	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4101	.	ribosome_entry_site	3535	3549	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4101	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I4101	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I4101	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I4101	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I4101	.	CDS	931	2058	.	+	0	ID=BBa_C0012;Name=BBa_C0012
BBa_I4101	.	engineered_tag	2059	2083	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_I4101	.	engineered_region	931	2058	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_I4101	.	CDS	931	2058	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_I4101	.	tag	2020	2058	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_I4101	.	terminator	3239	3318	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4101	.	engineered_region	3239	3318	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4101	.	stem_loop	3250	3293	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4101	.	terminator	713	792	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4101	.	engineered_region	713	792	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4101	.	stem_loop	724	767	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4101	.	promoter	3376	3526	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I4101	.	non_covalent_binding_site	3505	3524	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I4101	.	engineered_region	3376	3526	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I4101	.	promoter	3464	3469	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I4101	.	non_covalent_binding_site	3376	3395	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I4101	.	promoter	3490	3497	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I4101	.	start_codon	3503	3503	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I4101	.	CDS	2456	3205	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_I4101	.	tag	3167	3199	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_I4101	.	CDS	2459	3166	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_I4101	.	engineered_tag	3206	3230	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_I4101	.	promoter	850	903	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I4101	.	promoter	869	874	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I4101	.	non_covalent_binding_site	875	893	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I4101	.	engineered_region	850	903	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I4101	.	promoter	892	897	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I4101	.	non_covalent_binding_site	850	868	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I4101	.	promoter	2229	2428	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_I4101	.	non_covalent_binding_site	2317	2354	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_I4101	.	promoter	2389	2394	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_I4101	.	engineered_region	2229	2428	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_I4101	.	CDS	2229	2316	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_I4101	.	non_covalent_binding_site	2394	2428	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_I4101	.	promoter	2365	2370	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_I4101	.	start_codon	2401	2401	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_I4101	.	CDS	3556	4311	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_I4101	.	point_mutation	4047	4047	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I4101	.	CDS	3556	4305	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I4101	.	engineered_region	3556	4311	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I4101	.	engineered_tag	4312	4336	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I4101	.	point_mutation	3729	3729	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I4101	.	engineered_region	3556	3557	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I4101	.	terminator	4433	4473	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4101	.	engineered_region	4433	4473	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4101	.	stop_codon	4466	4466	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4101	.	stem_loop	4440	4459	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4101	.	polyA_site	4460	4473	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4101	.	ribosome_entry_site	912	924	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_I4101	.	ribosome_entry_site	918	921	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_I4101	.	ribosome_entry_site	912	924	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_I4101	.	engineered_region	912	924	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_I4101	.	CDS	20	679	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_I4101	.	engineered_tag	680	704	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_I4101	.	tag	640	673	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_I4101	.	CDS	23	639	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_I4101	.	terminator	2180	2220	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4101	.	engineered_region	2180	2220	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4101	.	stop_codon	2213	2213	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4101	.	stem_loop	2187	2206	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4101	.	polyA_site	2207	2220	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4101	.	terminator	3327	3367	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4101	.	engineered_region	3327	3367	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4101	.	stop_codon	3360	3360	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4101	.	stem_loop	3334	3353	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4101	.	polyA_site	3354	3367	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4101	.	terminator	2092	2171	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4101	.	engineered_region	2092	2171	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4101	.	stem_loop	2103	2146	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4101	.	terminator	801	841	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4101	.	engineered_region	801	841	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4101	.	stop_codon	834	834	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4101	.	stem_loop	808	827	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4101	.	polyA_site	828	841	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4101	.	terminator	4345	4424	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4101	.	engineered_region	4345	4424	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4101	.	stem_loop	4356	4399	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_I4101
tcacacaggaaagtactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtc
ggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaa
ataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaagggga
aagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagta
catttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggttttt
cactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatca
agtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgat
caccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaa
gtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagat
tgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaagtactagatggtgaatgtgaaacca
gtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacg
tttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaact
ggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcg
attaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaag
cggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgc
tgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttc
tcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcc
cattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagc
ggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccact
gcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcgg
atatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattt
tcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttg
cccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcat
taatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagcaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt
ttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttaca
ctttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcacacaggaaag
tactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaa
aaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgcttt
atttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaattt
agcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagt
accctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatg
ggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggc
tccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatag
ccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaaccc
acagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacg
tttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgc
ttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccatt
accgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactagagattaaagaggagaaatactag
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatc
aatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaa
atctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgat
cctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaat
ctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatgg
cttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacata
ccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacca
aaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtga
gcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagca
attttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagc
caggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
tactagagtcacactggctcaccttcgggtgggcctttctgcgtttata