##gff-version 3
##sequence-region BBa_I4103 1 2952
BBa_I4103	.	terminator	932	972	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4103	.	stop_codon	965	965	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4103	.	engineered_region	932	972	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4103	.	stem_loop	939	958	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4103	.	polyA_site	959	972	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4103	.	terminator	844	923	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4103	.	stem_loop	855	898	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4103	.	engineered_region	844	923	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4103	.	ribosome_entry_site	1	15	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4103	.	ribosome_entry_site	1	15	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4103	.	ribosome_entry_site	8	12	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4103	.	engineered_region	1	15	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4103	.	ribosome_entry_site	1043	1057	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4103	.	ribosome_entry_site	1043	1057	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4103	.	ribosome_entry_site	1050	1054	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4103	.	engineered_region	1043	1057	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4103	.	CDS	22	810	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_I4103	.	engineered_region	22	810	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_I4103	.	start_codon	22	24	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_I4103	.	engineered_tag	811	835	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_I4103	.	stop_codon	805	810	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_I4103	.	tag	772	804	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_I4103	.	CDS	22	771	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_I4103	.	terminator	1834	1913	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4103	.	stem_loop	1845	1888	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4103	.	engineered_region	1834	1913	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4103	.	engineered_region	2054	2815	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I4103	.	CDS	2054	2815	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I4103	.	engineered_region	2054	2815	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I4103	.	stop_codon	2810	2815	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I4103	.	sequence_alteration	2295	2295	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I4103	.	tag	2771	2809	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I4103	.	terminator	2824	2903	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I4103	.	stem_loop	2835	2878	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4103	.	engineered_region	2824	2903	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4103	.	ribosome_entry_site	2033	2047	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_I4103	.	ribosome_entry_site	2033	2047	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_I4103	.	ribosome_entry_site	2040	2044	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_I4103	.	engineered_region	2033	2047	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_I4103	.	terminator	2912	2952	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4103	.	stop_codon	2945	2945	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4103	.	engineered_region	2912	2952	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4103	.	stem_loop	2919	2938	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4103	.	polyA_site	2939	2952	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4103	.	promoter	1971	2024	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I4103	.	promoter	1990	1995	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I4103	.	non_covalent_binding_site	1996	2014	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I4103	.	engineered_region	1971	2024	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I4103	.	non_covalent_binding_site	1971	1989	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I4103	.	promoter	2013	2018	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I4103	.	terminator	1922	1962	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I4103	.	stop_codon	1955	1955	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4103	.	engineered_region	1922	1962	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4103	.	stem_loop	1929	1948	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4103	.	polyA_site	1949	1962	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4103	.	promoter	981	1034	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I4103	.	promoter	1000	1005	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I4103	.	non_covalent_binding_site	1006	1024	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I4103	.	engineered_region	981	1034	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I4103	.	non_covalent_binding_site	981	999	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I4103	.	promoter	1023	1028	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I4103	.	engineered_region	1064	1825	.	+	0	ID=BBa_E0022;Name=BBa_E0022
BBa_I4103	.	CDS	1064	1825	.	+	0	ID=annotation2150;Name=CFP (LVA);Parent=BBa_E0022
BBa_I4103	.	point_mutation	1132	1132	.	+	0	ID=annotation2155;Name=A;Parent=BBa_E0022
BBa_I4103	.	tag	1782	1819	.	+	0	ID=annotation2153;Name=SsrA;Parent=BBa_E0022
BBa_I4103	.	stop_codon	1820	1825	.	+	0	ID=annotation2154;Name=2;Parent=BBa_E0022
BBa_I4103	.	engineered_region	1064	1825	.	+	0	ID=annotation7040;Name=BBa_E0022;Parent=BBa_E0022
>BBa_I4103
attaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgt
ctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacc
tattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcag
gttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttcttt
atattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcg
tgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcatt
gaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaa
ttgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgcc
atttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgatt
gttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaact
acgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatac
tgagcactactagagattaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgccca
tcctggtcgagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacgg
caagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctgg
ggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacg
tccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccct
ggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactac
atcagccacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcg
aggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaa
ccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtg
accgccgccgggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtag
cttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtt
tgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccct
atcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaatactaga
tggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaa
gttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaag
ctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccacatga
agctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaa
ctacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaag
gaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcaga
agaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagca
gaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagac
cccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgt
acaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttata