LOCUS BBa_I4203 7323 bp DNA linear 16-Dec-2024 DEFINITION Prakash *: Synchronator Design 3 ACCESSION BBa_I4203 VERSION BBa_I4203.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..741 /label=BBa_C0040 stem_loop 786..829 /label=stem_loop misc_feature 775..854 /label=BBa_B0010 terminator 775..854 /label=BBa_B0010 stem_loop 870..889 /label=T7 TE misc_feature 896..896 /label=stop polyA_site 890..903 /label=polya misc_feature 863..903 /label=BBa_B0012 terminator 863..903 /label=BBa_B0012 misc_feature 912..930 /label=TetR 1 misc_feature 912..965 /label=BBa_R0040 promoter 931..936 /label=-35 misc_feature 937..955 /label=TetR 2 promoter 954..959 /label=-10 promoter 912..965 /label=BBa_R0040 misc_feature 64..965 /label=BBa_Q04400 misc_feature 978..981 /label=conserved RBS 974..985 /label=BBa_B0034 misc_feature 2081..2119 /label=LVA CDS 992..2119 /label=lacI-LVA misc_feature 992..2119 /label=BBa_C0012 misc_feature 2120..2144 /label=Help:Barcodes CDS 992..2119 /label=BBa_C0012 stem_loop 2164..2207 /label=stem_loop misc_feature 2153..2232 /label=BBa_B0010 terminator 2153..2232 /label=BBa_B0010 stem_loop 2248..2267 /label=T7 TE misc_feature 2274..2274 /label=stop polyA_site 2268..2281 /label=polya misc_feature 2241..2281 /label=BBa_B0012 terminator 2241..2281 /label=BBa_B0012 terminator 2153..2281 /label=BBa_B0015 misc_feature 2292..2308 /label=lac O1 promoter 2309..2314 /label=-35 misc_feature 2315..2331 /label=lac O1 promoter 2332..2337 /label=-10 misc_feature 2290..2343 /label=BBa_R0011 promoter 2290..2343 /label=BBa_R0011 misc_feature 974..2344 /label=BBa_Q04121 misc_feature 2357..2360 /label=conserved RBS 2353..2364 /label=BBa_B0034 CDS 2374..3081 /label=cI lambda misc_feature 3082..3114 /label=LVA misc_feature 3121..3145 /label=Help:Barcodes CDS 2371..3120 /label=BBa_C0051 stem_loop 3165..3208 /label=stem_loop misc_feature 3154..3233 /label=BBa_B0010 terminator 3154..3233 /label=BBa_B0010 stem_loop 3249..3268 /label=T7 TE misc_feature 3275..3275 /label=stop polyA_site 3269..3282 /label=polya misc_feature 3242..3282 /label=BBa_B0012 terminator 3242..3282 /label=BBa_B0012 misc_feature 3291..3387 /label=BBa_R0065 misc_feature 3296..3315 /label=Lux Box promoter 3337..3342 /label=-10 misc_feature 3347..3363 /label=OR2 cI promoter 3348..3348 /label=lux p(R) start promoter 3361..3366 /label=-35 misc_feature 3371..3387 /label=OR1 cI promoter 3384..3387 /label=-10 promoter 3291..3387 /label=BBa_R0065 misc_feature 2353..3387 /label=BBa_Q04511 misc_feature 3400..3403 /label=conserved RBS 3396..3407 /label=BBa_B0034 misc_feature 3993..4024 /label=LVA CDS 3414..3992 /label=luxI misc_feature 3414..4031 /label=BBa_C0061 misc_feature 4032..4056 /label=Help:Barcodes CDS 3414..4031 /label=BBa_C0061 stem_loop 4076..4119 /label=stem_loop misc_feature 4065..4144 /label=BBa_B0010 terminator 4065..4144 /label=BBa_B0010 stem_loop 4160..4179 /label=T7 TE misc_feature 4186..4186 /label=stop polyA_site 4180..4193 /label=polya misc_feature 4153..4193 /label=BBa_B0012 terminator 4153..4193 /label=BBa_B0012 misc_feature 3396..4193 /label=BBa_F1610 misc_feature 4202..4221 /label=LuxR/HSL promoter 4316..4323 /label=-10 promoter 4290..4295 /label=-35 misc_feature 4329..4329 /label=start misc_feature 4331..4350 /label=Putative LuxR/HSL misc_feature 4202..4352 /label=BBa_R0063 promoter 4202..4352 /label=BBa_R0063 misc_feature 4365..4368 /label=conserved RBS 4361..4372 /label=BBa_B0034 misc_feature 4379..4380 /label=prefix misc_feature 4552..4552 /label=T misc_feature 4870..4870 /label=A CDS 4379..5128 /label=luxR misc_feature 4379..5134 /label=BBa_C0062 misc_feature 5135..5159 /label=Help:Barcodes CDS 4379..5134 /label=BBa_C0062 stem_loop 5179..5222 /label=stem_loop misc_feature 5168..5247 /label=BBa_B0010 terminator 5168..5247 /label=BBa_B0010 stem_loop 5263..5282 /label=T7 TE misc_feature 5289..5289 /label=stop polyA_site 5283..5296 /label=polya misc_feature 5256..5296 /label=BBa_B0012 terminator 5256..5296 /label=BBa_B0012 misc_feature 4361..5296 /label=BBa_I0462 misc_feature 5307..5323 /label=lac O1 promoter 5324..5329 /label=-35 misc_feature 5330..5346 /label=lac O1 promoter 5347..5352 /label=-10 misc_feature 5305..5358 /label=BBa_R0011 promoter 5305..5358 /label=BBa_R0011 misc_feature 5372..5375 /label=conserved RBS 5368..5379 /label=BBa_B0034 misc_feature 5386..5388 /label=start misc_feature 6169..6174 /label=2 misc_feature 6136..6168 /label=LVA CDS 5386..6135 /label=aiiA misc_feature 5386..6174 /label=BBa_C0060 misc_feature 6175..6199 /label=Help:Barcodes CDS 5386..6174 /label=BBa_C0060 stem_loop 6219..6262 /label=stem_loop misc_feature 6208..6287 /label=BBa_B0010 terminator 6208..6287 /label=BBa_B0010 stem_loop 6303..6322 /label=T7 TE misc_feature 6329..6329 /label=stop polyA_site 6323..6336 /label=polya misc_feature 6296..6336 /label=BBa_B0012 terminator 6296..6336 /label=BBa_B0012 misc_feature 5368..6336 /label=BBa_I0460 misc_feature 6345..6363 /label=TetR 1 misc_feature 6345..6398 /label=BBa_R0040 promoter 6364..6369 /label=-35 misc_feature 6370..6388 /label=TetR 2 promoter 6387..6392 /label=-10 promoter 6345..6398 /label=BBa_R0040 misc_feature 6411..6414 /label=conserved RBS 6407..6418 /label=BBa_B0034 CDS 6425..7186 /label=YFP (LVA) misc_feature 7181..7186 /label=2 misc_feature 7142..7180 /label=SsrA misc_feature 6666..6666 /label=A (Q->L) misc_feature 6425..7186 /label=BBa_E0032 misc_feature 6425..7186 /label=BBa_E0032 stem_loop 7206..7249 /label=stem_loop misc_feature 7195..7274 /label=BBa_B0010 terminator 7195..7274 /label=BBa_B0010 stem_loop 7290..7309 /label=T7 TE misc_feature 7316..7316 /label=stop polyA_site 7310..7323 /label=polya misc_feature 7283..7323 /label=BBa_B0012 terminator 7283..7323 /label=BBa_B0012 misc_feature 6407..7323 /label=BBa_E0432 misc_feature 3396..7323 /label=BBa_I4204 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagccaggc 781 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 841 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 901 atatactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 961 agcactacta gagaaagagg agaaatacta gatggtgaat gtgaaaccag taacgttata 1021 cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc 1081 cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta 1141 cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc 1201 cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc 1261 cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg 1321 taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc 1381 gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt 1441 tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac 1501 gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg 1561 cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg 1621 caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca 1681 acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga 1741 tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga 1801 tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgttaac 1861 caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact 1921 ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa 1981 aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 2041 gcagctggca cgacaggttt cccgactgga aagcgggcag gctgcaaacg acgaaaacta 2101 cgctttagta gcttaataac tctgatagtg ctagtgtaga tctctactag agccaggcat 2161 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2221 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2281 atactagaga attgtgagcg gataacaatt gacattgtga gcggataaca agatactgag 2341 cacatactag agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa 2401 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt 2461 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct 2521 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc 2581 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa 2641 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt 2701 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg 2761 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc 2821 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac 2881 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt 2941 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag 3001 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag 3061 tggcctgaag agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa 3121 cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc 3181 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3241 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag taagcacctg 3301 taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac accgtgcgtg 3361 ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac tagatgacta 3421 taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa ggtattctaa 3481 gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta gaaaataacc 3541 ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt gatgatactg 3601 aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg ctgaaaagtg 3661 tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata gtcgaattaa 3721 gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt gaaattacaa 3781 tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca gaatatgtaa 3841 cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct tgtcatcgta 3901 ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg tctatgccta 3961 ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac tacgctttag 4021 tagcttaata actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa 4081 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 4141 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 4201 gacctgtacg atcctacagg tgcttatgtt aagtaattgt attcccagcg atacaatagt 4261 gtgacaaaaa tccaatttat tagaatcaaa tgtcaatcca ttaccgtttt aatgatatat 4321 aacacgcaaa acttgcgaca aacaataggt aatactagag aaagaggaga aatactagat 4381 gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag cttgtagaag 4441 caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt gtgaatatta 4501 tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa tcctagataa 4561 ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat atgatcctat 4621 agtagattat tctaactcca atcattcacc aattaattgg aatatatttg aaaacaatgc 4681 tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc ttatcactgg 4741 gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt ttgcacattc 4801 agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca taccattaat 4861 tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata aatcaaacaa 4921 cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa aaagctcttg 4981 ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt taaccaatgc 5041 gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa ttttaacagg 5101 agcaattgat tgcccatact ttaaaaatta ataacactga tagtgctagt gtagatcact 5161 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt 5221 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg 5281 cctttctgcg tttatatact agagaattgt gagcggataa caattgacat tgtgagcgga 5341 taacaagata ctgagcacat actagagaaa gaggagaaat actagatgac agtaaagaag 5401 ctttatttcg tcccagcagg tcgttgtatg ttggatcatt cgtctgttaa tagtacatta 5461 acaccaggag aattattaga cttaccggtt tggtgttatc ttttggagac tgaagaagga 5521 cctattttag tagatacagg tatgccagaa agtgcagtta ataatgaagg tctttttaac 5581 ggtacatttg tcgaagggca ggttttaccg aaaatgactg aagaagatag aatcgtgaat 5641 attttaaaac gggttggtta tgagccggaa gaccttcttt atattattag ttctcacttg 5701 cattttgatc atgcaggagg aaatggcgct tttataaata caccaatcat tgtacagcgt 5761 gctgaatatg aggcggcgca gcatagcgaa gaatatttga aagaatgtat attgccgaat 5821 ttaaactaca aaatcattga aggtgattat gaagtcgtac caggagttca attattgcat 5881 acaccaggcc atactccagg gcatcaatcg ctattaattg agacagaaaa atccggtcct 5941 gtattattaa cgattgatgc atcgtatacg aaagagaatt ttgaaaatga agtgccattt 6001 gcgggatttg attcagaatt agctttatct tcaattaaac gtttaaaaga agtggtgatg 6061 aaagagaagc cgattgtttt ctttggacat gatatagagc aggaaagggg atgtaaagtg 6121 ttccctgaat atatagctgc aaacgacgaa aactacgctt tagtagctta ataacgctga 6181 tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga 6241 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 6301 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta tcagtgatag 6361 agattgacat ccctatcagt gatagagata ctgagcacta ctagagaaag aggagaaata 6421 ctagatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 6481 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 6541 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 6601 caccctcgtg accaccttcg gctacggcct gcaatgcttc gcccgctacc ccgaccacat 6661 gaagctgcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 6721 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 6781 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 6841 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 6901 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 6961 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 7021 ccactacctg agctaccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 7081 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 7141 gaggcctgct gcaaacgacg aaaactacgc tttagtagct taataatact agagccaggc 7201 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 7261 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 7321 ata //