LOCUS BBa_I4204 3928 bp DNA linear 01-May-2025 DEFINITION Prakash *: Syncronator Cell-Signalling Module ACCESSION BBa_I4204 VERSION BBa_I4204.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 598..629 /label=LVA CDS 19..597 /label=luxI misc_feature 19..636 /label=BBa_C0061 misc_feature 637..661 /label=Help:Barcodes CDS 19..636 /label=BBa_C0061 stem_loop 681..724 /label=stem_loop misc_feature 670..749 /label=BBa_B0010 terminator 670..749 /label=BBa_B0010 stem_loop 765..784 /label=T7 TE misc_feature 791..791 /label=stop polyA_site 785..798 /label=polya misc_feature 758..798 /label=BBa_B0012 terminator 758..798 /label=BBa_B0012 misc_feature 1..798 /label=BBa_F1610 misc_feature 807..826 /label=LuxR/HSL promoter 921..928 /label=-10 promoter 895..900 /label=-35 misc_feature 934..934 /label=start misc_feature 936..955 /label=Putative LuxR/HSL misc_feature 807..957 /label=BBa_R0063 promoter 807..957 /label=BBa_R0063 misc_feature 970..973 /label=conserved RBS 966..977 /label=BBa_B0034 misc_feature 984..985 /label=prefix misc_feature 1157..1157 /label=T misc_feature 1475..1475 /label=A CDS 984..1733 /label=luxR misc_feature 984..1739 /label=BBa_C0062 misc_feature 1740..1764 /label=Help:Barcodes CDS 984..1739 /label=BBa_C0062 stem_loop 1784..1827 /label=stem_loop misc_feature 1773..1852 /label=BBa_B0010 terminator 1773..1852 /label=BBa_B0010 stem_loop 1868..1887 /label=T7 TE misc_feature 1894..1894 /label=stop polyA_site 1888..1901 /label=polya misc_feature 1861..1901 /label=BBa_B0012 terminator 1861..1901 /label=BBa_B0012 misc_feature 966..1901 /label=BBa_I0462 misc_feature 1912..1928 /label=lac O1 promoter 1929..1934 /label=-35 misc_feature 1935..1951 /label=lac O1 promoter 1952..1957 /label=-10 misc_feature 1910..1963 /label=BBa_R0011 promoter 1910..1963 /label=BBa_R0011 misc_feature 1977..1980 /label=conserved RBS 1973..1984 /label=BBa_B0034 misc_feature 1991..1993 /label=start misc_feature 2774..2779 /label=2 misc_feature 2741..2773 /label=LVA CDS 1991..2740 /label=aiiA misc_feature 1991..2779 /label=BBa_C0060 misc_feature 2780..2804 /label=Help:Barcodes CDS 1991..2779 /label=BBa_C0060 stem_loop 2824..2867 /label=stem_loop misc_feature 2813..2892 /label=BBa_B0010 terminator 2813..2892 /label=BBa_B0010 stem_loop 2908..2927 /label=T7 TE misc_feature 2934..2934 /label=stop polyA_site 2928..2941 /label=polya misc_feature 2901..2941 /label=BBa_B0012 terminator 2901..2941 /label=BBa_B0012 misc_feature 1973..2941 /label=BBa_I0460 misc_feature 2950..2968 /label=TetR 1 misc_feature 2950..3003 /label=BBa_R0040 promoter 2969..2974 /label=-35 misc_feature 2975..2993 /label=TetR 2 promoter 2992..2997 /label=-10 promoter 2950..3003 /label=BBa_R0040 misc_feature 3016..3019 /label=conserved RBS 3012..3023 /label=BBa_B0034 CDS 3030..3791 /label=YFP (LVA) misc_feature 3786..3791 /label=2 misc_feature 3747..3785 /label=SsrA misc_feature 3271..3271 /label=A (Q->L) misc_feature 3030..3791 /label=BBa_E0032 misc_feature 3030..3791 /label=BBa_E0032 stem_loop 3811..3854 /label=stem_loop misc_feature 3800..3879 /label=BBa_B0010 terminator 3800..3879 /label=BBa_B0010 stem_loop 3895..3914 /label=T7 TE misc_feature 3921..3921 /label=stop polyA_site 3915..3928 /label=polya misc_feature 3888..3928 /label=BBa_B0012 terminator 3888..3928 /label=BBa_B0012 misc_feature 3012..3928 /label=BBa_E0432 ORIGIN 1 aaagaggaga aatactagat gactataatg ataaaaaaat cggatttttt ggcaattcca 61 tcggaggagt ataaaggtat tctaagtctt cgttatcaag tgtttaagca aagacttgag 121 tgggacttag ttgtagaaaa taaccttgaa tcagatgagt atgataactc aaatgcagaa 181 tatatttatg cttgtgatga tactgaaaat gtaagtggat gctggcgttt attacctaca 241 acaggtgatt atatgctgaa aagtgttttt cctgaattgc ttggtcaaca gagtgctccc 301 aaagatccta atatagtcga attaagtcgt tttgctgtag gtaaaaatag ctcaaagata 361 aataactctg ctagtgaaat tacaatgaaa ctatttgaag ctatatataa acacgctgtt 421 agtcaaggta ttacagaata tgtaacagta acatcaacag caatagagcg atttttaaag 481 cgtattaaag ttccttgtca tcgtattgga gacaaagaaa ttcatgtatt aggtgatact 541 aaatcggttg tattgtctat gcctattaat gaacagttta aaaaagcagt cttaaatgct 601 gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta gtgtagatct 661 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 721 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 781 ggcctttctg cgtttatata ctagagacct gtacgatcct acaggtgctt atgttaagta 841 attgtattcc cagcgataca atagtgtgac aaaaatccaa tttattagaa tcaaatgtca 901 atccattacc gttttaatga tatataacac gcaaaacttg cgacaaacaa taggtaatac 961 tagagaaaga ggagaaatac tagatgaaaa acataaatgc cgacgacaca tacagaataa 1021 ttaataaaat taaagcttgt agaagcaata atgatattaa tcaatgctta tctgatatga 1081 ctaaaatggt acattgtgaa tattatttac tcgcgatcat ttatcctcat tctatggtta 1141 aatctgatat ttcaatccta gataattacc ctaaaaaatg gaggcaatat tatgatgacg 1201 ctaatttaat aaaatatgat cctatagtag attattctaa ctccaatcat tcaccaatta 1261 attggaatat atttgaaaac aatgctgtaa ataaaaaatc tccaaatgta attaaagaag 1321 cgaaaacatc aggtcttatc actgggttta gtttccctat tcatacggct aacaatggct 1381 tcggaatgct tagttttgca cattcagaaa aagacaacta tatagatagt ttatttttac 1441 atgcgtgtat gaacatacca ttaattgttc cttctctagt tgataattat cgaaaaataa 1501 atatagcaaa taataaatca aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt 1561 gggcatgcga aggaaaaagc tcttgggata tttcaaaaat attaggttgc agtgagcgta 1621 ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa 1681 gtatttctaa agcaatttta acaggagcaa ttgattgccc atactttaaa aattaataac 1741 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca 1801 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag 1861 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga attgtgagcg 1921 gataacaatt gacattgtga gcggataaca agatactgag cacatactag agaaagagga 1981 gaaatactag atgacagtaa agaagcttta tttcgtccca gcaggtcgtt gtatgttgga 2041 tcattcgtct gttaatagta cattaacacc aggagaatta ttagacttac cggtttggtg 2101 ttatcttttg gagactgaag aaggacctat tttagtagat acaggtatgc cagaaagtgc 2161 agttaataat gaaggtcttt ttaacggtac atttgtcgaa gggcaggttt taccgaaaat 2221 gactgaagaa gatagaatcg tgaatatttt aaaacgggtt ggttatgagc cggaagacct 2281 tctttatatt attagttctc acttgcattt tgatcatgca ggaggaaatg gcgcttttat 2341 aaatacacca atcattgtac agcgtgctga atatgaggcg gcgcagcata gcgaagaata 2401 tttgaaagaa tgtatattgc cgaatttaaa ctacaaaatc attgaaggtg attatgaagt 2461 cgtaccagga gttcaattat tgcatacacc aggccatact ccagggcatc aatcgctatt 2521 aattgagaca gaaaaatccg gtcctgtatt attaacgatt gatgcatcgt atacgaaaga 2581 gaattttgaa aatgaagtgc catttgcggg atttgattca gaattagctt tatcttcaat 2641 taaacgttta aaagaagtgg tgatgaaaga gaagccgatt gttttctttg gacatgatat 2701 agagcaggaa aggggatgta aagtgttccc tgaatatata gctgcaaacg acgaaaacta 2761 cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag agccaggcat 2821 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 2881 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 2941 atactagagt ccctatcagt gatagagatt gacatcccta tcagtgatag agatactgag 3001 cactactaga gaaagaggag aaatactaga tggtgagcaa gggcgaggag ctgttcaccg 3061 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 3121 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca 3181 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcctgcaat 3241 gcttcgcccg ctaccccgac cacatgaagc tgcacgactt cttcaagtcc gccatgcccg 3301 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 3361 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 3421 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 3481 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca 3541 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 3601 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 3661 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 3721 ctctcggcat ggacgagctg tacaagaggc ctgctgcaaa cgacgaaaac tacgctttag 3781 tagcttaata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 3841 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 3901 ccttcgggtg ggcctttctg cgtttata //