##gff-version 3
##sequence-region BBa_I4204 1 3928
BBa_I4204	.	engineered_region	1973	2941	.	+	0	ID=BBa_I0460;Name=BBa_I0460
BBa_I4204	.	terminator	2813	2892	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0460
BBa_I4204	.	stem_loop	2824	2867	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4204	.	engineered_region	2813	2892	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4204	.	ribosome_entry_site	1973	1984	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0460
BBa_I4204	.	conserved_region	1977	1980	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I4204	.	CDS	1991	2779	.	+	0	ID=BBa_C0060;Name=BBa_C0060;Parent=BBa_I0460
BBa_I4204	.	CDS	1991	2740	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_I4204	.	start_codon	1991	1993	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_I4204	.	engineered_region	1991	2779	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_I4204	.	engineered_tag	2780	2804	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_I4204	.	tag	2741	2773	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_I4204	.	stop_codon	2774	2779	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_I4204	.	terminator	2901	2941	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0460
BBa_I4204	.	stem_loop	2908	2927	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4204	.	stop_codon	2934	2934	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4204	.	polyA_site	2928	2941	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4204	.	engineered_region	2901	2941	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4204	.	engineered_region	1	798	.	+	0	ID=BBa_F1610;Name=BBa_F1610
BBa_I4204	.	terminator	758	798	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F1610
BBa_I4204	.	stem_loop	765	784	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4204	.	stop_codon	791	791	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4204	.	polyA_site	785	798	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4204	.	engineered_region	758	798	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4204	.	terminator	670	749	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F1610
BBa_I4204	.	stem_loop	681	724	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4204	.	engineered_region	670	749	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4204	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F1610
BBa_I4204	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I4204	.	CDS	19	636	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_F1610
BBa_I4204	.	CDS	19	597	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_I4204	.	engineered_tag	637	661	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_I4204	.	engineered_region	19	636	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_I4204	.	tag	598	629	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_I4204	.	promoter	807	957	.	+	0	ID=BBa_R0063;Name=BBa_R0063
BBa_I4204	.	promoter	895	900	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_I4204	.	promoter	921	928	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_I4204	.	non_covalent_binding_site	807	826	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_I4204	.	non_covalent_binding_site	936	955	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_I4204	.	engineered_region	807	957	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_I4204	.	start_codon	934	934	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_I4204	.	engineered_region	3012	3928	.	+	0	ID=BBa_E0432;Name=BBa_E0432
BBa_I4204	.	terminator	3800	3879	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0432
BBa_I4204	.	stem_loop	3811	3854	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4204	.	engineered_region	3800	3879	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I4204	.	ribosome_entry_site	3012	3023	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0432
BBa_I4204	.	conserved_region	3016	3019	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I4204	.	engineered_region	3030	3791	.	+	0	ID=BBa_E0032;Name=BBa_E0032;Parent=BBa_E0432
BBa_I4204	.	CDS	3030	3791	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I4204	.	sequence_alteration	3271	3271	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I4204	.	engineered_region	3030	3791	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I4204	.	stop_codon	3786	3791	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I4204	.	tag	3747	3785	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I4204	.	terminator	3888	3928	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0432
BBa_I4204	.	stem_loop	3895	3914	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4204	.	stop_codon	3921	3921	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4204	.	polyA_site	3915	3928	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4204	.	engineered_region	3888	3928	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4204	.	promoter	2950	3003	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I4204	.	engineered_region	2950	3003	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I4204	.	promoter	2969	2974	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I4204	.	promoter	2992	2997	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I4204	.	non_covalent_binding_site	2950	2968	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I4204	.	non_covalent_binding_site	2975	2993	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I4204	.	promoter	1910	1963	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I4204	.	promoter	1952	1957	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I4204	.	promoter	1929	1934	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I4204	.	operator	1912	1928	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I4204	.	engineered_region	1910	1963	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I4204	.	operator	1935	1951	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I4204	.	engineered_region	966	1901	.	+	0	ID=BBa_I0462;Name=BBa_I0462
BBa_I4204	.	terminator	1861	1901	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0462
BBa_I4204	.	stem_loop	1868	1887	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I4204	.	stop_codon	1894	1894	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I4204	.	polyA_site	1888	1901	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I4204	.	engineered_region	1861	1901	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I4204	.	CDS	984	1739	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I0462
BBa_I4204	.	point_mutation	1475	1475	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_I4204	.	engineered_region	984	1739	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_I4204	.	engineered_region	984	985	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_I4204	.	point_mutation	1157	1157	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_I4204	.	CDS	984	1733	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_I4204	.	engineered_tag	1740	1764	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_I4204	.	ribosome_entry_site	966	977	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0462
BBa_I4204	.	conserved_region	970	973	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I4204	.	terminator	1773	1852	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0462
BBa_I4204	.	stem_loop	1784	1827	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I4204	.	engineered_region	1773	1852	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_I4204
aaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataaaggta
ttctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatcagatga
gtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttattacct
acaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaatatag
tcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaactatt
tgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagcgattt
ttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggttgtat
tgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagtagctta
ataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtga
caaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaac
aataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataa
aattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattat
ttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggc
aatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattg
gaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcact
gggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactata
tagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaa
tatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagc
tcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactca
atacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaata
acactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcct
ttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgt
ttatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaa
gaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgtta
atagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctatttt
agtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggtttta
ccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatatta
ttagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctga
atatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggt
gattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgaga
cagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgc
gggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttc
tttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctt
tagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtg
ggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagca
ctactagagaaagaggagaaatactagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcg
agctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgac
cctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaa
tgcttcgcccgctaccccgaccacatgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagc
gcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccg
catcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccac
aacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggca
gcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacct
gagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgcc
gggatcactctcggcatggacgagctgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataat
actagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga
acgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata