LOCUS BBa_I4212 6330 bp DNA linear 26-Dec-2024 DEFINITION Synchronator design 2, Output- ACCESSION BBa_I4212 VERSION BBa_I4212.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 CDS 127..743 /label=tetR misc_feature 744..777 /label=SsrA misc_feature 784..808 /label=Help:Barcodes CDS 124..783 /label=BBa_C0040 stem_loop 828..871 /label=stem_loop misc_feature 817..896 /label=BBa_B0010 terminator 817..896 /label=BBa_B0010 stem_loop 912..931 /label=T7 TE misc_feature 938..938 /label=stop polyA_site 932..945 /label=polya misc_feature 905..945 /label=BBa_B0012 terminator 905..945 /label=BBa_B0012 misc_feature 954..972 /label=TetR 1 misc_feature 954..1007 /label=BBa_R0040 promoter 973..978 /label=-35 misc_feature 979..997 /label=TetR 2 promoter 996..1001 /label=-10 promoter 954..1007 /label=BBa_R0040 misc_feature 106..1007 /label=BBa_Q04400 misc_feature 1020..1023 /label=conserved RBS 1016..1027 /label=BBa_B0034 misc_feature 2123..2161 /label=LVA CDS 1034..2161 /label=lacI-LVA misc_feature 1034..2161 /label=BBa_C0012 misc_feature 2162..2186 /label=Help:Barcodes CDS 1034..2161 /label=BBa_C0012 stem_loop 2206..2249 /label=stem_loop misc_feature 2195..2274 /label=BBa_B0010 terminator 2195..2274 /label=BBa_B0010 stem_loop 2290..2309 /label=T7 TE misc_feature 2316..2316 /label=stop polyA_site 2310..2323 /label=polya misc_feature 2283..2323 /label=BBa_B0012 terminator 2283..2323 /label=BBa_B0012 terminator 2195..2323 /label=BBa_B0015 misc_feature 2334..2350 /label=lac O1 promoter 2351..2356 /label=-35 misc_feature 2357..2373 /label=lac O1 promoter 2374..2379 /label=-10 misc_feature 2332..2385 /label=BBa_R0011 promoter 2332..2385 /label=BBa_R0011 misc_feature 1016..2386 /label=BBa_Q04121 misc_feature 2399..2402 /label=conserved RBS 2395..2406 /label=BBa_B0034 CDS 2416..3123 /label=cI lambda misc_feature 3124..3156 /label=LVA misc_feature 3163..3187 /label=Help:Barcodes CDS 2413..3162 /label=BBa_C0051 stem_loop 3207..3250 /label=stem_loop misc_feature 3196..3275 /label=BBa_B0010 terminator 3196..3275 /label=BBa_B0010 stem_loop 3291..3310 /label=T7 TE misc_feature 3317..3317 /label=stop polyA_site 3311..3324 /label=polya misc_feature 3284..3324 /label=BBa_B0012 terminator 3284..3324 /label=BBa_B0012 promoter 3370..3375 /label=-10 promoter 3347..3352 /label=-35 misc_feature 3357..3373 /label=OR1 misc_feature 3333..3349 /label=OR2 misc_feature 3333..3381 /label=BBa_R0051 promoter 3333..3381 /label=BBa_R0051 misc_feature 2395..3381 /label=BBa_Q04510 misc_feature 3394..3397 /label=conserved RBS 3390..3401 /label=BBa_B0034 misc_feature 3987..4018 /label=LVA CDS 3408..3986 /label=luxI misc_feature 3408..4025 /label=BBa_C0061 misc_feature 4026..4050 /label=Help:Barcodes CDS 3408..4025 /label=BBa_C0061 stem_loop 4070..4113 /label=stem_loop misc_feature 4059..4138 /label=BBa_B0010 terminator 4059..4138 /label=BBa_B0010 stem_loop 4154..4173 /label=T7 TE misc_feature 4180..4180 /label=stop polyA_site 4174..4187 /label=polya misc_feature 4147..4187 /label=BBa_B0012 terminator 4147..4187 /label=BBa_B0012 misc_feature 3390..4187 /label=BBa_F1610 misc_feature 4196..4215 /label=LuxR/HSL promoter 4310..4317 /label=-10 promoter 4284..4289 /label=-35 misc_feature 4323..4323 /label=start misc_feature 4325..4344 /label=Putative LuxR/HSL misc_feature 4196..4346 /label=BBa_R0063 promoter 4196..4346 /label=BBa_R0063 misc_feature 4359..4362 /label=conserved RBS 4355..4366 /label=BBa_B0034 misc_feature 4373..4374 /label=prefix misc_feature 4546..4546 /label=T misc_feature 4864..4864 /label=A CDS 4373..5122 /label=luxR misc_feature 4373..5128 /label=BBa_C0062 misc_feature 5129..5153 /label=Help:Barcodes CDS 4373..5128 /label=BBa_C0062 stem_loop 5173..5216 /label=stem_loop misc_feature 5162..5241 /label=BBa_B0010 terminator 5162..5241 /label=BBa_B0010 stem_loop 5257..5276 /label=T7 TE misc_feature 5283..5283 /label=stop polyA_site 5277..5290 /label=polya misc_feature 5250..5290 /label=BBa_B0012 terminator 5250..5290 /label=BBa_B0012 misc_feature 4355..5290 /label=BBa_I0462 misc_feature 5301..5317 /label=lac O1 promoter 5318..5323 /label=-35 misc_feature 5324..5340 /label=lac O1 promoter 5341..5346 /label=-10 misc_feature 5299..5352 /label=BBa_R0011 promoter 5299..5352 /label=BBa_R0011 misc_feature 5366..5369 /label=conserved RBS 5362..5373 /label=BBa_B0034 misc_feature 5380..5382 /label=start misc_feature 6163..6168 /label=2 misc_feature 6130..6162 /label=LVA CDS 5380..6129 /label=aiiA misc_feature 5380..6168 /label=BBa_C0060 misc_feature 6169..6193 /label=Help:Barcodes CDS 5380..6168 /label=BBa_C0060 stem_loop 6213..6256 /label=stem_loop misc_feature 6202..6281 /label=BBa_B0010 terminator 6202..6281 /label=BBa_B0010 stem_loop 6297..6316 /label=T7 TE misc_feature 6323..6323 /label=stop polyA_site 6317..6330 /label=polya misc_feature 6290..6330 /label=BBa_B0012 terminator 6290..6330 /label=BBa_B0012 misc_feature 5362..6330 /label=BBa_I0460 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatgtcca gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag 181 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agctaggtgt agagcagcct 241 acattgtatt ggcatgtaaa aaataagcgg gctttgctcg acgccttagc cattgagatg 301 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta 361 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta 421 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt 481 ttatgccaac aaggtttttc actagagaat gcattatatg cactcagcgc tgtggggcat 541 tttactttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa 601 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac 661 caaggtgcag agccagcctt cttattcggc cttgaattga tcatatgcgg attagaaaaa 721 caacttaaat gtgaaagtgg gtccgctgca aacgacgaaa actacgcttt agtagcttaa 781 taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg 841 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 901 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat 961 cagtgataga gattgacatc cctatcagtg atagagatac tgagcactac tagagaaaga 1021 ggagaaatac tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc 1081 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa 1141 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc 1201 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct 1261 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag 1321 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa 1381 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc 1441 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca 1501 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca 1561 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc 1621 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat 1681 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct 1741 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc 1801 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata 1861 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt 1921 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt 1981 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa 2041 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 2101 ttcccgactg gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata 2161 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct 2221 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 2281 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gaattgtgag 2341 cggataacaa ttgacattgt gagcggataa caagatactg agcacatact agagaaagag 2401 gagaaatact agatgagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca 2461 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct 2521 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat 2581 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa 2641 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg 2701 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca 2761 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa 2821 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc 2881 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag 2941 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc 3001 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc 3061 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt 3121 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt 3181 agatcgctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 3241 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 3301 cgggtgggcc tttctgcgtt tatatactag agtaacaccg tgcgtgttga ctattttacc 3361 tctggcggtg ataatggttg ctactagaga aagaggagaa atactagatg actataatga 3421 taaaaaaatc ggattttttg gcaattccat cggaggagta taaaggtatt ctaagtcttc 3481 gttatcaagt gtttaagcaa agacttgagt gggacttagt tgtagaaaat aaccttgaat 3541 cagatgagta tgataactca aatgcagaat atatttatgc ttgtgatgat actgaaaatg 3601 taagtggatg ctggcgttta ttacctacaa caggtgatta tatgctgaaa agtgtttttc 3661 ctgaattgct tggtcaacag agtgctccca aagatcctaa tatagtcgaa ttaagtcgtt 3721 ttgctgtagg taaaaatagc tcaaagataa ataactctgc tagtgaaatt acaatgaaac 3781 tatttgaagc tatatataaa cacgctgtta gtcaaggtat tacagaatat gtaacagtaa 3841 catcaacagc aatagagcga tttttaaagc gtattaaagt tccttgtcat cgtattggag 3901 acaaagaaat tcatgtatta ggtgatacta aatcggttgt attgtctatg cctattaatg 3961 aacagtttaa aaaagcagtc ttaaatgctg caaacgacga aaactacgct ttagtagctt 4021 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa 4081 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4141 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg 4201 tacgatccta caggtgctta tgttaagtaa ttgtattccc agcgatacaa tagtgtgaca 4261 aaaatccaat ttattagaat caaatgtcaa tccattaccg ttttaatgat atataacacg 4321 caaaacttgc gacaaacaat aggtaatact agagaaagag gagaaatact agatgaaaaa 4381 cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa 4441 tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact 4501 cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc 4561 taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga 4621 ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa 4681 taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag 4741 tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa 4801 agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc 4861 ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt 4921 aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat 4981 ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat 5041 gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat 5101 tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga 5161 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 5221 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 5281 tgcgtttata tactagagaa ttgtgagcgg ataacaattg acattgtgag cggataacaa 5341 gatactgagc acatactaga gaaagaggag aaatactaga tgacagtaaa gaagctttat 5401 ttcgtcccag caggtcgttg tatgttggat cattcgtctg ttaatagtac attaacacca 5461 ggagaattat tagacttacc ggtttggtgt tatcttttgg agactgaaga aggacctatt 5521 ttagtagata caggtatgcc agaaagtgca gttaataatg aaggtctttt taacggtaca 5581 tttgtcgaag ggcaggtttt accgaaaatg actgaagaag atagaatcgt gaatatttta 5641 aaacgggttg gttatgagcc ggaagacctt ctttatatta ttagttctca cttgcatttt 5701 gatcatgcag gaggaaatgg cgcttttata aatacaccaa tcattgtaca gcgtgctgaa 5761 tatgaggcgg cgcagcatag cgaagaatat ttgaaagaat gtatattgcc gaatttaaac 5821 tacaaaatca ttgaaggtga ttatgaagtc gtaccaggag ttcaattatt gcatacacca 5881 ggccatactc cagggcatca atcgctatta attgagacag aaaaatccgg tcctgtatta 5941 ttaacgattg atgcatcgta tacgaaagag aattttgaaa atgaagtgcc atttgcggga 6001 tttgattcag aattagcttt atcttcaatt aaacgtttaa aagaagtggt gatgaaagag 6061 aagccgattg ttttctttgg acatgatata gagcaggaaa ggggatgtaa agtgttccct 6121 gaatatatag ctgcaaacga cgaaaactac gctttagtag cttaataacg ctgatagtgc 6181 tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact 6241 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct 6301 caccttcggg tgggcctttc tgcgtttata //