LOCUS       BBa_I5000                953 bp    DNA     linear       22-Dec-2024
DEFINITION  Olmecs: Inducible expression of HK022 cI repressor
ACCESSION   BBa_I5000
VERSION     BBa_I5000.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    820..825
                     /label=2
     CDS             82..825
                     /label=cI HK022
     misc_feature    787..819
                     /label=SsrA
     misc_feature    82..89
                     /label=OR3 partial
     misc_feature    82..825
                     /label=BBa_C0050
     misc_feature    826..850
                     /label=Help:Barcodes
     CDS             82..825
                     /label=BBa_C0050
     stem_loop       866..885
                     /label=T7 TE
     misc_feature    892..892
                     /label=stop
     polyA_site      886..899
                     /label=polya
     misc_feature    859..899
                     /label=BBa_B0012
     terminator      859..899
                     /label=BBa_B0012
     stem_loop       920..942
                     /label=stem_loop
     misc_feature    908..953
                     /label=BBa_B0011
     terminator      908..953
                     /label=BBa_B0011
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatggttcaa cagaaagagc gtgaaacttt ctcgcagagg
      121 cttgcgctgg cctgtgataa agcgggatta cctttgcatg gtaggcaggc tgatttagct
      181 gtcaggctta aggtcacacc aaaagccatt agtaaatggt tcaacgggga gtcaatacca
      241 agaaaagaca agatggaatc tctggcttcg gtgctgggaa ctactgctgc atatctgcat
      301 ggctatgctg atgatgacgg tatcacggta aatcatctat caagatcaaa tgattattat
      361 cgtgttgatg tattggatgt tcaggcgagc gccgggccag gaaccatggt ttccaatgaa
      421 tttatagaaa agataagagc aattgaatat acgaccgagc aggcaagaat tttatttaat
      481 ggaaggccac aggaaagcgt aaaagtcatc acggttcgcg gtgacagcat ggagggaacc
      541 atcaatccgg gagatgagat ctttgttgat gtatccataa cctgttttga tggcgatggc
      601 atttatgtgt ttgtatacgg gaaaacaatg cacgttaagc gcctgcaaat gcaaaagaac
      661 aggcttgccg tcatctctga caatgccgct tatgatcgat ggtacataga agaaggtgaa
      721 gaagagcaac ttcacattct agccaaagtc ctcattaggc agtcaatcga ttacaagcga
      781 ttcggagctg caaacgacga aaactacgct ttagtagctt aataaccctg atagtgctag
      841 tgtagatccc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      901 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta ttt
//