LOCUS BBa_I50000 4640 bp DNA linear 04-May-2024 DEFINITION F plasmid backbone with BioBrick sites removed. ACCESSION BBa_I50000 VERSION BBa_I50000.1 FEATURES Location/Qualifiers misc_feature 3938..4640 /label=sopC(IncD) repeat region CDS 2893..3864 /label=parB CDS 1718..2893 /label=parA misc_feature 1..1390 /label=sequence derived from F plasmid in GenBank Accession Number AP001918 CDS 384..1139 /label=repE misc_feature 1..290 /label=ori2 (oriS) single copy replication origin misc_feature 2553..2553 /label=C to T mutation (eliminates PstI site) misc_feature 3711..3711 /label=A to T mutation (eliminates SpeI site) misc_feature 1013..1013 /label=C to T mutation (eliminates PstI site) misc_feature 183..183 /label=C to G mutation (eliminates XbaI site, noncoding region) misc_feature 89..97 /label=DnaA box misc_feature 101..147 /label=A/T region misc_feature 192..211 /label=iteron (repeat region) misc_feature 213..232 /label=iteron (repeat region) misc_feature 246..265 /label=iteron (repeat region) misc_feature 268..287 /label=iteron (repeat region) misc_feature 81..88 /label=DnaA box misc_feature 1720..4640 /label=sequence derived from F plasmid in GenBank Accession Number AP001918 ORIGIN 1 tcgagtgagc gaggaagcac cagggaacag cacttatata ttctgcttac acacgatgcc 61 tgaaaaaact tcccttgggg ttatccactt atccacgggg atatttttat aattattttt 121 tttatagttt ttagatcttc ttttttagag cgccttgtag gcctttatcc atgctggttc 181 tacagaaggt gttgtgacaa attgcccttt cagtgtgaca aatcaccctc aaatgacagt 241 cctgtctgtg acaaattgcc cttaaccctg tgacaaattg ccctcagaag aagctgtttt 301 ttcacaaagt tatccctgct tattgactct tttttattta gtgtgacaat ctaaaaactt 361 gtcacacttc acatggatct gtcatggcgg aaacagcggt tatcaatcac aagaaacgta 421 aaaatagccc gcgaatcgtc cagtcaaacg acctcactga ggcggcatat agtctctccc 481 gggatcaaaa acgtatgctg tatctgttcg ttgaccagat cagaaaatct gatggcaccc 541 tacaggaaca tgacggtatc tgcgagatcc atgttgctaa atatgctgaa atattcggat 601 tgacctctgc ggaagccagt aaggatatac ggcaggcatt gaagagtttc gcggggaagg 661 aagtggtttt ttatcgccct gaagaggatg ccggcgatga aaaaggctat gaatcttttc 721 cttggtttat caaacgtgcg cacagtccat ccagagggct ttacagtgta catatcaacc 781 catatctcat tcccttcttt atcgggttac agaaccggtt tacgcagttt cggcttagtg 841 aaacaaaaga aatcaccaat ccgtatgcca tgcgtttata cgaatccctg tgtcagtatc 901 gtaagccgga tggctcaggc atcgtctctc tgaaaatcga ctggatcata gagcgttacc 961 agctgcctca aagttaccag cgtatgcctg acttccgccg ccgcttcctg caagtctgtg 1021 ttaatgagat caacagcaga actccaatgc gcctctcata cattgagaaa aagaaaggcc 1081 gccagacgac tcatatcgta ttttccttcc gcgatatcac ttccatgacg acaggatagt 1141 ctgagggtta tctgtcacag atttgagggt ggttcgtcac atttgttctg acctactgag 1201 ggtaatttgt cacagttttg ctgtttcctt cagcctgcat ggattttctc atactttttg 1261 aactgtaatt tttaaggaag ccaaatttga gggcagtttg tcacagttga tttccttctc 1321 tttcccttcg tcatgtgact tgatatcggg ggttagttcg tcatcattga tgagggttga 1381 ttatcacagt ttattactct gaattggcta tccgcgtgtg tacctctacc tggagttttt 1441 cccacggtgg atatttcttc ttgcgctgag cgtaagagct atctgacaga acagttcttc 1501 tttgcttcct cgccagttcg ctcgctatgc tcggttacac ggctgcggcg agcgctagtg 1561 ataataagtg actgaggtat gtgctcttct tatctccttt tgtagtgttg ctcttatttt 1621 aaacaacttt gcggtttttt gatgactttg cgattttgtt gttgctttgc agtaaattgc 1681 aagatttaat aaaaaaacgc aaagcaatga ttaaaggatg ttcagaatga aactcatgga 1741 aacacttaac cagtgcataa acgctggtca tgaaatgacg aaggctatcg ccattgcaca 1801 gtttaatgat gacagcccgg aagcgaggaa aataacccgg cgctggagaa taggtgaagc 1861 agcggattta gttggggttt cttctcaggc tatcagagat gccgagaaag cagggcgact 1921 accgcacccg gatatggaaa ttcgaggacg ggttgagcaa cgtgttggtt atacaattga 1981 acaaattaat catatgcgtg atgtgtttgg tacgcgattg cgacgtgctg aagacgtatt 2041 tccaccggtg atcggggttg ctgcccataa aggtggcgtt tacaaaacct cagtttctgt 2101 tcatcttgct caggatctgg ctctgaaggg gctacgtgtt ttgctcgtgg aaggtaacga 2161 cccccaggga acagcctcaa tgtatcacgg atgggtacca gatcttcata ttcatgcaga 2221 agacactctc ctgcctttct atcttgggga aaaggacgat gtcacttatg caataaagcc 2281 cacttgctgg ccggggcttg acattattcc ttcctgtctg gctctgcacc gtattgaaac 2341 tgagttaatg ggcaaatttg atgaaggtaa actgcccacc gatccacacc tgatgctccg 2401 actggccatt gaaactgttg ctcatgacta tgatgtcata gttattgaca gcgcgcctaa 2461 cctgggtatc ggcacgatta atgtcgtatg tgctgctgat gtgctgattg ttcccacgcc 2521 tgctgagttg tttgactaca cctccgcact gcaatttttc gatatgcttc gtgatctgct 2581 caagaacgtt gatcttaaag ggttcgagcc tgatgtacgt attttgctta ccaaatacag 2641 caatagtaat ggctctcagt ccccgtggat ggaggagcaa attcgggatg cctggggaag 2701 catggttcta aaaaatgttg tacgtgaaac ggatgaagtt ggtaaaggtc agatccggat 2761 gagaactgtt tttgaacagg ccattgatca acgctcttca actggtgcct ggagaaatgc 2821 tctttctatt tgggaacctg tctgcaatga aattttcgat cgtctgatta aaccacgctg 2881 ggagattaga taatgaagcg tgcgcctgtt attccaaaac atacgctcaa tactcaaccg 2941 gttgaagata cttcgttatc gacaccagct gccccgatgg tggattcgtt aattgcgcgc 3001 gtaggagtaa tggctcgcgg taatgccatt actttgcctg tatgtggtcg ggatgtgaag 3061 tttactcttg aagtgctccg gggtgatagt gttgagaaga cctctcgggt atggtcaggt 3121 aatgaacgtg accaggagct gcttactgag gacgcactgg atgatctcat cccttctttt 3181 ctactgactg gtcaacagac accggcgttc ggtcgaagag tatctggtgt catagaaatt 3241 gccgatggga gtcgccgtcg taaagctgct gcacttaccg aaagtgatta tcgtgttctg 3301 gttggcgagc tggatgatga gcagatggct gcattatcca gattgggtaa cgattatcgc 3361 ccaacaagtg cttatgaacg tggtcagcgt tatgcaagcc gattgcagaa tgaatttgct 3421 ggaaatattt ctgcgctggc tgatgcggaa aatatttcac gtaagattat tacccgctgt 3481 atcaacaccg ccaaattgcc taaatcagtt gttgctcttt tttctcaccc cggtgaacta 3541 tctgcccggt caggtgatgc acttcaaaaa gcctttacag ataaagagga attacttaag 3601 cagcaggcat ctaaccttca tgagcagaaa aaagctgggg tgatatttga agctgaagaa 3661 gttatcactc ttttaacttc tgtgcttaaa acgtcatctg catcaagaac aagtttaagc 3721 tcacgacatc agtttgctcc tggagcgaca gtattgtata agggcgataa aatggtgctt 3781 aacctggaca ggtctcgtgt tccaactgag tgtatagaga aaattgaggc cattcttaag 3841 gaacttgaaa agccagcacc ctgatgcgac cacgttttag tctacgttta tctgtcttta 3901 cttaatgtcc tttgttacag gccagaaagc ataactggcc tgaatattct ctctgggccc 3961 actgttccac ttgtatcgtc ggtctgataa tcagactggg accacggtcc cactcgtatc 4021 gtcggtctga ttattagtct gggaccacgg tcccactcgt atcgtcggtc tgattattag 4081 tctgggacca cggtcccact cgtatcgtcg gtctgataat cagactggga ccacggtccc 4141 actcgtatcg tcggtctgat tattagtctg ggaccatggt cccactcgta tcgtcggtct 4201 gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctggaac 4261 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat 4321 cgtcggtctg attattagtc tgggaccacg atcccactcg tgttgtcggt ctgattatcg 4381 gtctgggacc acggtcccac ttgtattgtc gatcagacta tcagcgtgag actacgattc 4441 catcaatgcc tgtcaagggc aagtattgac atgtcgtcgt aacctgtaga acggagtaac 4501 ctcggtgtgc ggttgtatgc ctgctgtgga ttgctgctgt gtcctgctta tccacaacat 4561 tttgcgcacg gttatgtgga caaaatacct ggttacccag gccgtgccgg cacgttcacc 4621 gggctgcatc cgatgcaagt //