LOCUS       BBa_I50040              2226 bp    DNA     linear       26-Dec-2024
DEFINITION  nonfunctional pSC101 origin of replication
ACCESSION   BBa_I50040
VERSION     BBa_I50040.1  
FEATURES             Location/Qualifiers
     misc_feature    180..189
                     /label=inverted repeat
     misc_feature    1..368
                     /label=par region
     misc_feature    191..201
                     /label=inverted repeat
     misc_feature    482..492
                     /label=11bp sequence repeated 4 times in oriC
     misc_feature    503..581
                     /label=AT rich region
     misc_feature    578..601
                     /label=direct repeat
     misc_feature    603..620
                     /label=direct repeat
     misc_feature    631..654
                     /label=direct repeat
     misc_feature    702..723
                     /label=inverted repeat
     promoter        710..729
                     /label=promoter region
     stem_loop       683..761
                     /label=potential hairpin
     promoter        723..727
                     /label=-10 signal functional promoter
     RBS             734..746
                     /label=RBS
     misc_feature    726..749
                     /label=inverted repeat
     promoter        948..953
                     /label=-10 signal functional promoter
     CDS             750..1700
                     /label=repA
     stem_loop       1608..1682
                     /label=potential hairpin
     stem_loop       2063..2088
                     /label=potential hairpin
     misc_feature    708..708
                     /label=A->T mutation to remove MfeI site
     misc_feature    742..742
                     /label=T->A mutation to remove MfeI site
ORIGIN
        1 gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactggctgc attagccagt
       61 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa
      121 ctgctaaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga
      181 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag
      241 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat
      301 gtcacgaaaa agacagcgat tcaggtgcct gatggtcgga gacaaaagga atattcagcg
      361 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta
      421 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg
      481 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat
      541 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag
      601 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca
      661 caactcaaag gaaaaggaca tgtaattatc attgactagc ccatctctat tggtatagtg
      721 attaaaatca cctagactaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg
      781 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg
      841 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca
      901 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag
      961 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag
     1021 gctttgagat tttccaatgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag
     1081 tttttagtga ggagatattg ccttatcttt tccagttaaa aaagttcata aaatataatc
     1141 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa
     1201 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgagttta
     1261 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt
     1321 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc
     1381 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggacctcg
     1441 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta
     1501 catcagattc ctacctacgt aacggactaa gaaaaacact acacgatgct ttaactgcaa
     1561 aaatccagct caccagtttt gaggcaaagt ttttgagtga catgcaaagt aagcatgatc
     1621 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg
     1681 ctaaatacgg aaggatttaa ggttcttatg gctcttgtat ctatcagtga agcatcaaga
     1741 ctaacaaaca aaagtagaac aactgttcac cgttagatat caaagggaaa actgtccaaa
     1801 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt
     1861 gcatttaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca
     1921 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg
     1981 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc catgctgctt
     2041 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca
     2101 tggcaattct ggaagaaata gcgctttcag cgggcaaacc tgaaaccgaa tctgcgattc
     2161 tgataacaaa ctagcaacac cagaacagcc cgtttgcggg cagcaaaacc cgtacacctg
     2221 ggtatc
//