BBa_I50040 1 pSC101 nonfunctional pSC101 origin of replication 2006-02-03T12:00:00Z 2015-08-31T04:07:42Z pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. false false _41_6_ 0 126 45 It's complicated false false Reshma Shetty annotation1796226 1 direct repeat range1796226 1 578 601 annotation1796223 1 inverted repeat range1796223 1 191 201 annotation1796232 1 -10 signal functional promoter range1796232 1 723 727 annotation1796228 1 direct repeat range1796228 1 631 654 annotation1880623 1 A->T mutation to remove MfeI site range1880623 1 708 708 annotation1796231 1 potential hairpin range1796231 1 683 761 annotation1796221 1 inverted repeat range1796221 1 180 189 annotation1796235 1 -10 signal functional promoter range1796235 1 948 953 annotation1796227 1 direct repeat range1796227 1 603 620 annotation1796237 1 potential hairpin range1796237 1 1608 1682 annotation1796238 1 potential hairpin range1796238 1 2063 2088 annotation1796225 1 AT rich region range1796225 1 503 581 annotation1796222 1 par region range1796222 1 1 368 annotation1796230 1 promoter region range1796230 1 710 729 annotation1796224 1 11bp sequence repeated 4 times in oriC range1796224 1 482 492 annotation1796236 1 repA range1796236 1 750 1700 annotation1796233 1 RBS range1796233 1 734 746 annotation1796234 1 inverted repeat range1796234 1 726 749 annotation1880624 1 T->A mutation to remove MfeI site range1880624 1 742 742 annotation1796229 1 inverted repeat range1796229 1 702 723 BBa_I50040_sequence 1 gacagtaagacgggtaagcctgttgatgataccgctgccttactggctgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctaaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgattcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctctattggtatagtgattaaaatcacctagactaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccaatggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaggagatattgccttatcttttccagttaaaaaagttcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgagtttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggacctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacgtaacggactaagaaaaacactacacgatgctttaactgcaaaaatccagctcaccagttttgaggcaaagtttttgagtgacatgcaaagtaagcatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatttaaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttagatatcaaagggaaaactgtccaaatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcatttaaagctgttcaccatgaacagatcgacaatgtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagtaaaacaaagcaactagaacatgaaattgaacacctgagacaacttgttacagctcaacagtcacacatagacagcctgaaacaggccatgctgcttatcgaatcaaagctgccgacaacacgggagccagtgacgcctcccgtggggaaaaaatcatggcaattctggaagaaatagcgctttcagcgggcaaacctgaaaccgaatctgcgattctgataacaaactagcaacaccagaacagcccgtttgcgggcagcaaaacccgtacacctgggtatc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z