##gff-version 3
##sequence-region BBa_I50040 1 2088
BBa_I50040	.	promoter	710	729	.	+	0	ID=annotation1796230;Name=promoter region
BBa_I50040	.	sequence_feature	503	581	.	+	0	ID=annotation1796225;Name=AT rich region
BBa_I50040	.	sequence_feature	578	601	.	+	0	ID=annotation1796226;Name=direct repeat
BBa_I50040	.	sequence_feature	631	654	.	+	0	ID=annotation1796228;Name=direct repeat
BBa_I50040	.	promoter	723	727	.	+	0	ID=annotation1796232;Name=-10 signal functional promoter
BBa_I50040	.	sequence_alteration	742	742	.	+	0	ID=annotation1880624;Name=T->A mutation to remove MfeI site
BBa_I50040	.	stem_loop	683	761	.	+	0	ID=annotation1796231;Name=potential hairpin
BBa_I50040	.	sequence_feature	603	620	.	+	0	ID=annotation1796227;Name=direct repeat
BBa_I50040	.	sequence_feature	180	189	.	+	0	ID=annotation1796221;Name=inverted repeat
BBa_I50040	.	sequence_feature	482	492	.	+	0	ID=annotation1796224;Name=11bp sequence repeated 4 times in oriC
BBa_I50040	.	stem_loop	2063	2088	.	+	0	ID=annotation1796238;Name=potential hairpin
BBa_I50040	.	stem_loop	1608	1682	.	+	0	ID=annotation1796237;Name=potential hairpin
BBa_I50040	.	sequence_feature	191	201	.	+	0	ID=annotation1796223;Name=inverted repeat
BBa_I50040	.	CDS	750	1700	.	+	0	ID=annotation1796236;Name=repA
BBa_I50040	.	ribosome_entry_site	734	746	.	+	0	ID=annotation1796233;Name=RBS
BBa_I50040	.	sequence_alteration	708	708	.	+	0	ID=annotation1880623;Name=A->T mutation to remove MfeI site
BBa_I50040	.	sequence_feature	726	749	.	+	0	ID=annotation1796234;Name=inverted repeat
BBa_I50040	.	sequence_feature	1	368	.	+	0	ID=annotation1796222;Name=par region
BBa_I50040	.	promoter	948	953	.	+	0	ID=annotation1796235;Name=-10 signal functional promoter
BBa_I50040	.	sequence_feature	702	723	.	+	0	ID=annotation1796229;Name=inverted repeat
>BBa_I50040
gacagtaagacgggtaagcctgttgatgataccgctgccttactggctgcattagccagtctgaatgacctgtcacggg
ataatccgaagtggtcagactggaaaatcagagggcaggaactgctaaacagcaaaaagtcagatagcaccacatagca
gacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataa
aaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacag
cgattcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcct
ttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaa
gtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccac
ttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagca
aaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctctatt
ggtatagtgattaaaatcacctagactaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgat
tagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaac
cctacaaggaaagaacggacggtatcgttcacttataaccaatacgctcagatgatgaacatcagtagggaaaatgctt
atggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttga
gattttccaatggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaggagatattgccttat
cttttccagttaaaaaagttcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggattt
atgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgagtttaagtt
catgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagattta
aacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactag
atagacaaatggacctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattac
atcagattcctacctacgtaacggactaagaaaaacactacacgatgctttaactgcaaaaatccagctcaccagtttt
gaggcaaagtttttgagtgacatgcaaagtaagcatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaa
ccacactagagaacatactggctaaatacggaaggatttaaggttcttatggctcttgtatctatcagtgaagcatcaa
gactaacaaacaaaagtagaacaactgttcaccgttagatatcaaagggaaaactgtccaaatgcacagatgaaaacgg
tgtaaaaaagatagatacatcagagcttttacgagtttttggtgcatttaaagctgttcaccatgaacagatcgacaat
gtaacagatgaacagcatgtaacacctaatagaacaggtgaaaccagtaaaacaaagcaactagaacatgaaattgaac
acctgagacaacttgttacagctcaacagtcacacatagacagcctgaaacaggccatgctgcttatcgaatcaaagct
gccgacaacacgggagccagtgacgcctcccgtggggaaaaaatcatggcaattctggaagaaatagcgctttcagcgg
gcaaacctgaaaccgaatctgcgattctgataacaaactagcaacaccagaacagcccgtttgcgggcagcaaaacccg
tacacctgggtatc