LOCUS       BBa_I50041              2037 bp    DNA     linear       19-Apr-2024
DEFINITION  pSC101 origin of replication, reverse
ACCESSION   BBa_I50041
VERSION     BBa_I50041.1  
FEATURES             Location/Qualifiers
     polyA_site      76..91
                     /label=putative polyA
     CDS             202..1152
                     /label=repA
     misc_feature    1154..1176
                     /label=inverted repeat
     RBS             1154..1159
                     /label=RBS
     RBS             1156..1168
                     /label=RBS
     promoter        1173..1192
                     /label=promoter region
     misc_feature    1179..1200
                     /label=inverted repeat
     promoter        1183..1188
                     /label=rep promoter -10
     promoter        1205..1210
                     /label=rep promoter -35
     misc_feature    1219..1224
                     /label=Mutated SpeI site
     misc_feature    1250..1269
                     /label=direct repeat
     misc_feature    1282..1301
                     /label=direct repeat
     misc_feature    1303..1322
                     /label=direct repeat
     misc_feature    1321..1403
                     /label=AT rich region
     misc_feature    1534..1901
                     /label=par region
     misc_feature    1800..1817
                     /label=inverted repeat
     misc_feature    1823..1846
                     /label=inverted repeat
     misc_feature    1857..1873
                     /label=inverted repeat
     misc_feature    1876..1880
                     /label=Tn3 integration site
     misc_feature    1886..1899
                     /label=inverted repeat
     stem_loop       1963..1992
                     /label=terminator
     misc_feature    36..36
                     /label=difference from canonical pSC101 sequence in 
                     Genbank
     misc_feature    126..126
                     /label=difference from canonical pSC101 sequence in 
                     Genbank
     misc_feature    288..288
                     /label=difference from canonical pSC101 sequence in 
                     Genbank
     misc_feature    383..383
                     /label=difference from canonical pSC101 sequence in 
                     Genbank
ORIGIN
        1 gttacattgt cgatctgttc atggtgaaca gctttgaatg caccaaaaac tcgtaaaagc
       61 tctgatgtat ctatcttttt tacaccgttt tcatctgtgc atatggacag ttttcccttt
      121 gatatgtaac ggtgaacagt tgttctactt ttgtttgtta gtcttgatgc ttcactgata
      181 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt
      241 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat
      301 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg
      361 tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt
      421 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc
      481 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt
      541 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct
      601 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat
      661 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat
      721 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt
      781 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct
      841 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct
      901 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt
      961 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc
     1021 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt
     1081 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac
     1141 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg
     1201 ggctagtcaa tgataattac atgtcctttt cctttgagtt gtgggtatct gtaaattctg
     1261 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc
     1321 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata
     1381 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc
     1441 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa
     1501 ccctaaaggc ttaagtagca ccctcgcaag ctcgggcaaa tcgctgaata ttccttttgt
     1561 ctccgaccat caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca
     1621 cggctctggc agtgaatggg ggtaaatggc actacaggcg ccttttatgg attcatgcaa
     1681 ggaaactacc cataatacaa gaaaagcccg tcacgggctt ctcagggcgt tttatggcgg
     1741 gtctgctatg tggtgctatc tgactttttg ctgttcagca gttcctgccc tctgattttc
     1801 cagtctgacc acttcggatt atcccgtgac aggtcattca gactggctaa tgcacccagt
     1861 aaggcagcgg tatcatcaac aggcttaccc gtcttactgt ccctagtgct tggattctca
     1921 ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag
     1981 gtcattactg gatctatcaa caggagtcca agcgagctcg taaacttggt ctgacag
//