BBa_I50041 1 pSC101 pSC101 origin of replication, reverse 2006-03-13T12:00:00Z 2015-08-31T04:07:42Z pSC101 origin of replication which is supposed to have low copy number. This was the origin used in Michael Elowitz's repressilator and in Bujard's pZS4Int-1 vector with mutations introduced to eliminate a SpeI site. It is also the origin of replication in pSB4* series plasmids. This part is the reverse copy of BBa_I50040. false false _41_6_ 0 126 45 Not in stock false false Reshma Shetty annotation2080857 1 difference from canonical pSC101 sequence in Genbank range2080857 1 126 126 annotation1932235 1 terminator range1932235 1 1963 1992 annotation1932225 1 direct repeat range1932225 1 1250 1269 annotation1932216 1 repA range1932216 1 202 1152 annotation1932226 1 direct repeat range1932226 1 1282 1301 annotation2080859 1 difference from canonical pSC101 sequence in Genbank range2080859 1 383 383 annotation1932223 1 rep promoter -35 range1932223 1 1205 1210 annotation1932233 1 Tn3 integration site range1932233 1 1876 1880 annotation1932228 1 AT rich region range1932228 1 1321 1403 annotation1932220 1 promoter region range1932220 1 1173 1192 annotation2080858 1 difference from canonical pSC101 sequence in Genbank range2080858 1 288 288 annotation1932219 1 RBS range1932219 1 1156 1168 annotation1932231 1 inverted repeat range1932231 1 1823 1846 annotation1932222 1 rep promoter -10 range1932222 1 1183 1188 annotation1932229 1 par region range1932229 1 1534 1901 annotation1932218 1 RBS range1932218 1 1154 1159 annotation1932227 1 direct repeat range1932227 1 1303 1322 annotation1932217 1 inverted repeat range1932217 1 1154 1176 annotation1932221 1 inverted repeat range1932221 1 1179 1200 annotation1932215 1 putative polyA range1932215 1 76 91 annotation2080856 1 difference from canonical pSC101 sequence in Genbank range2080856 1 36 36 annotation1932232 1 inverted repeat range1932232 1 1857 1873 annotation1932230 1 inverted repeat range1932230 1 1800 1817 annotation1932234 1 inverted repeat range1932234 1 1886 1899 annotation1932224 1 Mutated SpeI site range1932224 1 1219 1224 BBa_I50041_sequence 1 gttacattgtcgatctgttcatggtgaacagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatcttttttacaccgttttcatctgtgcatatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgttagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggcgggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcggattatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtcttactgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z