##gff-version 3
##sequence-region BBa_I50041 36 1992
BBa_I50041	.	sequence_feature	1250	1269	.	+	0	ID=annotation1932225;Name=direct repeat
BBa_I50041	.	promoter	1183	1188	.	+	0	ID=annotation1932222;Name=rep promoter -10
BBa_I50041	.	sequence_alteration	36	36	.	+	0	ID=annotation2080856;Name=difference from canonical pSC101 sequence in Genbank
BBa_I50041	.	sequence_feature	1857	1873	.	+	0	ID=annotation1932232;Name=inverted repeat
BBa_I50041	.	sequence_feature	1800	1817	.	+	0	ID=annotation1932230;Name=inverted repeat
BBa_I50041	.	sequence_feature	1303	1322	.	+	0	ID=annotation1932227;Name=direct repeat
BBa_I50041	.	sequence_alteration	383	383	.	+	0	ID=annotation2080859;Name=difference from canonical pSC101 sequence in Genbank
BBa_I50041	.	sequence_feature	1823	1846	.	+	0	ID=annotation1932231;Name=inverted repeat
BBa_I50041	.	sequence_alteration	288	288	.	+	0	ID=annotation2080858;Name=difference from canonical pSC101 sequence in Genbank
BBa_I50041	.	sequence_feature	1282	1301	.	+	0	ID=annotation1932226;Name=direct repeat
BBa_I50041	.	sequence_feature	1886	1899	.	+	0	ID=annotation1932234;Name=inverted repeat
BBa_I50041	.	sequence_feature	1321	1403	.	+	0	ID=annotation1932228;Name=AT rich region
BBa_I50041	.	sequence_alteration	126	126	.	+	0	ID=annotation2080857;Name=difference from canonical pSC101 sequence in Genbank
BBa_I50041	.	sequence_feature	1534	1901	.	+	0	ID=annotation1932229;Name=par region
BBa_I50041	.	ribosome_entry_site	1154	1159	.	+	0	ID=annotation1932218;Name=RBS
BBa_I50041	.	CDS	202	1152	.	+	0	ID=annotation1932216;Name=repA
BBa_I50041	.	promoter	1205	1210	.	+	0	ID=annotation1932223;Name=rep promoter -35
BBa_I50041	.	sequence_feature	1876	1880	.	+	0	ID=annotation1932233;Name=Tn3 integration site
BBa_I50041	.	sequence_feature	1154	1176	.	+	0	ID=annotation1932217;Name=inverted repeat
BBa_I50041	.	ribosome_entry_site	1156	1168	.	+	0	ID=annotation1932219;Name=RBS
BBa_I50041	.	promoter	1173	1192	.	+	0	ID=annotation1932220;Name=promoter region
BBa_I50041	.	stem_loop	1963	1992	.	+	0	ID=annotation1932235;Name=terminator
BBa_I50041	.	sequence_feature	1179	1200	.	+	0	ID=annotation1932221;Name=inverted repeat
BBa_I50041	.	sequence_alteration	1219	1224	.	+	0	ID=annotation1932224;Name=Mutated SpeI site
BBa_I50041	.	polyA_site	76	91	.	+	0	ID=annotation1932215;Name=putative polyA
>BBa_I50041
gttacattgtcgatctgttcatggtgaacagctttgaatgcaccaaaaactcgtaaaagctctgatgtatctatctttt
ttacaccgttttcatctgtgcatatggacagttttccctttgatatgtaacggtgaacagttgttctacttttgtttgt
tagtcttgatgcttcactgatagatacaagagccataagaacctcagatccttccgtatttagccagtatgttctctag
tgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaatttt
gcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaat
ctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttg
tctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaa
gtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcatta
acatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataacca
ctcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactgg
aaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactgga
aaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatac
accataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttcctt
gtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagc
gactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcact
ataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctag
acctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttata
ttcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactc
actactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaac
cctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctg
agtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggc
gccttttatggattcatgcaaggaaactacccataatacaagaaaagcccgtcacgggcttctcagggcgttttatggc
gggtctgctatgtggtgctatctgactttttgctgttcagcagttcctgccctctgattttccagtctgaccacttcgg
attatcccgtgacaggtcattcagactggctaatgcacccagtaaggcagcggtatcatcaacaggcttacccgtctta
ctgtccctagtgcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagtt
ctgaggtcattactggatctatcaacaggagtccaagcgagctcgtaaacttggtctgacag