BBa_I50052 1 pSC101 ts pSC101 temperature sensitive origin of replication 2010-06-20T11:00:00Z 2015-08-31T04:07:42Z This part is derived from BBa_I50042. It contains a point mutation that changes Alanine to Valine in RepA protein. BBa_I50052 is a pSC101 temperature sensitive origin of replication. BBa_I50052 is the temperature sensitive replication origin found in the pSB8* series of BioBrick vectors as well as the pSIM5 recombineering vector. false false _41_ 0 126 162 Not in stock false See design notes for [[Part:BBa_I50042]]. false Reshma Shetty annotation2071361 1 G to C difference with canonical pSC101 vector sequence range2071361 1 1912 1912 annotation2071339 1 Tn3 integration site range2071339 1 158 162 annotation2071347 1 mutated SpeI site range2071347 1 814 819 annotation2071350 1 promoter region range2071350 1 846 865 annotation2071343 1 AT rich region range2071343 1 635 717 annotation2071356 1 inverted repeat range2071356 1 1847 1861 annotation2071358 1 G to A difference with canonical pSC101 vector sequence range2071358 1 1655 1655 annotation2071348 1 -35 range2071348 1 828 833 annotation2071340 1 inverted repeat range2071340 1 165 181 annotation2071349 1 inverted repeat range2071349 1 838 859 annotation2071353 1 RBS range2071353 1 870 882 annotation2071362 1 T to C difference with canonical pSC101 vector sequence range2071362 1 2002 2002 annotation2071342 1 inverted repeat range2071342 1 221 238 annotation2071355 1 repA range2071355 1 886 1836 annotation2071336 1 Terminator range2071336 1 46 75 annotation2071351 1 -10 range2071351 1 850 855 annotation2071352 1 inverted repeat range2071352 1 862 884 annotation2071341 1 inverted repeat range2071341 1 192 215 annotation2071344 1 direct repeat range2071344 1 716 735 annotation2071346 1 direct repeat range2071346 1 769 788 annotation2071345 1 direct repeat range2071345 1 737 756 annotation2071360 1 C to T difference with canonical pSC101 vector sequence range2071360 1 1750 1750 annotation2071354 1 RBS range2071354 1 879 884 annotation2071337 1 par region range2071337 1 137 504 annotation2071338 1 inverted repeat range2071338 1 139 152 annotation2071359 1 putative polyA range2071359 1 1947 1962 annotation2071357 1 GCT->GTT ts mutation (Ala->Val) range2071357 1 1051 1053 BBa_I50052_sequence 1 ctgtcagaccaagtttacgagctcgcttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccaagcactagggacagtaagacgggtaagcctgttgatgataccgctgccttactgggtgcattagccagtctgaatgacctgtcacgggataatccgaagtggtcagactggaaaatcagagggcaggaactgctgaacagcaaaaagtcagatagcaccacatagcagacccgccataaaacgccctgagaagcccgtgacgggcttttcttgtattatgggtagtttccttgcatgaatccataaaaggcgcctgtagtgccatttacccccattcactgccagagccgtgagcgcagcgaactgaatgtcacgaaaaagacagcgactcaggtgcctgatggtcggagacaaaaggaatattcagcgatttgcccgagcttgcgagggtgctacttaagcctttagggttttaaggtctgttttgtagaggagcaaacagcgtttgcgacatccttttgtaatactgcggaactgactaaagtagtgagttatacacagggctgggatctattctttttatctttttttattctttctttattctataaattataaccacttgaatataaacaaaaaaaacacacaaaggtctagcggaatttacagagggtctagcagaatttacaagttttccagcaaaggtctagcagaatttacagatacccacaactcaaaggaaaaggacatgtaattatcattgactagcccatctcaattggtatagtgattaaaatcacctagaccaattgagatgtatgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgttcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctgaggttcttatggctcttgtatctatcagtgaagcatcaagactaacaaacaaaagtagaacaactgttcaccgttacatatcaaagggaaaactgtccatatgcacagatgaaaacggtgtaaaaaagatagatacatcagagcttttacgagtttttggtgcattcaaagctgttcaccatgaacagatcgacaatgtaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z