LOCUS       BBa_I5030                896 bp    DNA     linear       14-May-2024
DEFINITION  Olmecs: Inducible expression of P22 c2 repressor
ACCESSION   BBa_I5030
VERSION     BBa_I5030.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..729
                     /label=cII p22
     misc_feature    730..762
                     /label=LVA
     misc_feature    763..768
                     /label=stop
     misc_feature    82..768
                     /label=BBa_C0053
     misc_feature    769..793
                     /label=Help:Barcodes
     CDS             82..768
                     /label=BBa_C0053
     stem_loop       809..828
                     /label=T7 TE
     misc_feature    835..835
                     /label=stop
     polyA_site      829..842
                     /label=polya
     misc_feature    802..842
                     /label=BBa_B0012
     terminator      802..842
                     /label=BBa_B0012
     stem_loop       863..885
                     /label=stem_loop
     misc_feature    851..896
                     /label=BBa_B0011
     terminator      851..896
                     /label=BBa_B0011
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaataca caattgatgg gtgagcgtat tcgcgctcga
      121 agaaaaaaac tcaagattag acaagccgct cttggtaaga tggtgggagt gtctaatgtt
      181 gcaatatcgc aatgggagcg ctcggagact gagccaaatg gggagaacct gttggcactt
      241 tcgaaggctc ttcagtgctc ccctgactat ttgctgaaag gagatttaag ccagacaaac
      301 gttgcctatc atagtaggca tgagccaaga ggatcatacc ctcttatcag ttgggtaagc
      361 gcagggcaat ggatggaagc tgtagaacct tatcacaagc gcgcgataga gaactggcac
      421 gacaccactg tagattgttc agaagattca ttttggcttg atgtccaagg tgactctatg
      481 acagcaccgg cagggttaag cattccagaa ggaatgataa ttctggttga tcccgaagtc
      541 gaaccaagaa acggcaagct ggttgttgca aaattagaag gtgaaaacga ggccacattc
      601 aaaaaattag ttatggatgc aggccgaaag tttttaaaac cattaaaccc acaatatccg
      661 atgatagaaa tcaacggaaa ctgcaaaatc attggcgtag ttgttgacgc aaaactcgca
      721 aatcttccag ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc
      781 tagtgtagat cactactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      841 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttattt
//