##gff-version 3
##sequence-region BBa_I5030 1 896
BBa_I5030	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I5030	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I5030	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I5030	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I5030	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I5030	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I5030	.	CDS	82	768	.	+	0	ID=BBa_C0053;Name=BBa_C0053
BBa_I5030	.	stop_codon	763	768	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_I5030	.	engineered_region	82	768	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_I5030	.	tag	730	762	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_I5030	.	engineered_tag	769	793	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_I5030	.	CDS	82	729	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_I5030	.	terminator	802	842	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5030	.	stem_loop	809	828	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5030	.	stop_codon	835	835	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5030	.	polyA_site	829	842	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I5030	.	engineered_region	802	842	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5030	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5030	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5030	.	terminator	851	896	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5030	.	engineered_region	851	896	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5030	.	stem_loop	863	885	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
>BBa_I5030
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatgaatacacaattgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaa
gatggtgggagtgtctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggca
ctttcgaaggctcttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagta
ggcatgagccaagaggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatca
caagcgcgcgatagagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgac
tctatgacagcaccggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacg
gcaagctggttgttgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtt
tttaaaaccattaaacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgca
aaactcgcaaatcttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagat
cactactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccag
attattaatccggcttttttattattt