LOCUS       BBa_I5100                946 bp    DNA     linear       21-Dec-2024
DEFINITION  HK022 cI regulatable expression of YFP
ACCESSION   BBa_I5100
VERSION     BBa_I5100.1  
FEATURES             Location/Qualifiers
     promoter        8..13
                     /label=-35
     RBS             13..27
                     /label=OR2
     promoter        30..35
                     /label=-10
     RBS             37..51
                     /label=OR1
     misc_feature    43..43
                     /label=putative
     misc_feature    1..55
                     /label=BBa_R0050
     promoter        1..55
                     /label=BBa_R0050
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..843
                     /label=YFP (LVA)
     misc_feature    838..843
                     /label=2
     misc_feature    799..837
                     /label=SsrA
     misc_feature    323..323
                     /label=A (Q->L)
     misc_feature    82..843
                     /label=BBa_E0032
     misc_feature    82..843
                     /label=BBa_E0032
     stem_loop       859..878
                     /label=T7 TE
     misc_feature    885..885
                     /label=stop
     polyA_site      879..892
                     /label=polya
     misc_feature    852..892
                     /label=BBa_B0012
     terminator      852..892
                     /label=BBa_B0012
     stem_loop       913..935
                     /label=stem_loop
     misc_feature    901..946
                     /label=BBa_B0011
     terminator      901..946
                     /label=BBa_B0011
ORIGIN
        1 ttaggtattg actgtactat cagttccgtc ataatatgaa ccataagttc accactacta
       61 gagaaagagg agaaatacta gatggtgagc aagggcgagg agctgttcac cggggtggtg
      121 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      181 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      241 ctgcccgtgc cctggcccac cctcgtgacc accttcggct acggcctgca atgcttcgcc
      301 cgctaccccg accacatgaa gctgcacgac ttcttcaagt ccgccatgcc cgaaggctac
      361 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      421 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      481 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      541 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      601 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      661 gtgctgctgc ccgacaacca ctacctgagc taccagtccg ccctgagcaa agaccccaac
      721 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      781 atggacgagc tgtacaagag gcctgctgca aacgacgaaa actacgcttt agtagcttaa
      841 taatactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
      901 agagaatata aaaagccaga ttattaatcc ggctttttta ttattt
//