LOCUS BBa_I51000 2446 bp DNA linear 02-May-2024 DEFINITION BioBrick base vector, as originally designed ACCESSION BBa_I51000 VERSION BBa_I51000.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..182 /label=NheI site misc_feature 177..182 /label=BBa_B0045 CDS 183..1046 /label=ampicillin resistance RBS 1051..1055 /label=ampR RBS promoter 1064..1069 /label=ampR -10 promoter 1090..1095 /label=ampR -35 misc_feature 183..1125 /label=BBa_P1006 misc_feature 1126..1131 /label=NheI site misc_feature 1126..1131 /label=BBa_B0045 stem_loop 1140..1174 /label=stem_loop terminator 1132..1203 /label=BBa_B0053 primer_bind 1204..1223 /label=VF2 primer 1204..1223 /label=BBa_G00100 stem_loop 1259..1280 /label=stem loop from bacteriophage T3 misc_feature 1251..1289 /label=identical to bacteriophage T3 terminator 1224..1301 /label=BBa_B0055 misc_feature 1303..1305 /label=stop codon (+2 frame) misc_feature 1302..1304 /label=stop codon (-1 frame) misc_feature 1307..1309 /label=stop codon (+3 frame) misc_feature 1311..1313 /label=stop codon (+1 frame) misc_feature 1310..1312 /label=stop codon (-2 frame) misc_feature 1306..1308 /label=stop codon (-3 frame) misc_feature 1302..1313 /label=BBa_B0042 misc_feature 1314..1318 /label=Topoisomerase I recognition site misc_feature 1322..1326 /label=Nt.BstNB I recognition site misc_feature 1319..1321 /label=Arbitrary sequence (could be any bases) misc_feature 1318..1318 /label=Generated T overhang misc_feature 1314..1326 /label=BBa_B0043 misc_feature 1327..1332 /label=EcoRI site misc_feature 1342..1347 /label=XbaI site misc_feature 1327..1348 /label=Prefix misc_feature 1333..1340 /label=NotI site misc_feature 1327..1348 /label=BBa_G00000 promoter 1389..1394 /label=-35 promoter 1412..1417 /label=-10 CDS 1456..1764 /label=ccdB misc_feature 1349..1764 /label=BBa_P1011 misc_feature 1788..2402 /label=rep (pMB1) misc_feature 2387..2387 /label=ORI misc_feature 1799..2387 /label=origin of replication misc_feature 1835..2387 /label=RNAII transcript promoter 1976..1981 /label=RNAI transcript promoter -35 promoter 1954..1959 /label=RNAI transcript promoter -10 misc_feature 1838..1945 /label=RNAI transcript promoter 1820..1825 /label=RNAII transcript promoter -10 sequence promoter 1799..1804 /label=RNAII transcript promoter -35 sequence misc_feature 2033..2033 /label=G to A, nonfunctional mutation misc_feature 2037..2037 /label=G to A, nonfunctional mutation misc_feature 2046..2046 /label=G to A, nonfunctional mutation misc_feature 2075..2075 /label=C to T, nonfunctional mutation misc_feature 2239..2239 /label=C to T, nonfunctional mutation misc_feature 1773..2446 /label=BBa_I50020 misc_feature 1349..2446 /label=BBa_I52000 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gcttattacc aatgcttaat cagggaggca cctatctcag cgatctggcg attacgttcg 241 tccatagttg cctggctccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 301 ggccccaggg ctgcaataat accgcgggac ccacgctcac cggctccaga tttatcagca 361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 421 atccagtcta ttaattgttg ccgggaagcg agagtaagaa gttcgccagt taagagtttg 481 cgcaacgttg ttgccatagc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcacccat gttgtgcaaa 601 aaagcggtaa gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgccgtgtta 661 tcactcatgg ttatggcagc gctgcataat tcgcgtactg tcatgccgtc cgtaagatgc 721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacagagcag aactttaaaa 841 gtgctcatca ttgggaaacg ttcttcgggg cgaaaactct caagaatctt accgctgttg 901 aggtccagtt cgatgtaacc cacacgtgca cccaactgat cttcggcatc ttttactttc 961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 1021 gcgacacgga aatgttgaat actcatattc ttcctttttc aatattattg aagcatttat 1081 cagggttatt gtctcatgag cggatagata tttgaatgta tttaggctag ctccggcaaa 1141 aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc 1201 gtttgccacc tgacgtctaa gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg 1261 cccacccgtg aaggtgagcc agtgagttga ttgctacgta attagttagt tagcccttag 1321 tgactcgaat tcgcggccgc ttctagaggg cttactaaaa gccagataac agtatgcgta 1381 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc 1441 aaaaagaggt atgctatgca gtttaaggtt tacacctata aacgtgagag ccgttatcgt 1501 ctgtttgtgg acgtacagag tgatattatt gacacgcctg ggcgtcgcat ggtgattccc 1561 ctggccagcg cacgtctgct ttcagataaa gtctcccgtg aactttaccc ggtggtgcat 1621 atcggggacg aaagctggcg catgatgacc accgatatgg ccagcgtgcc ggtttccgtg 1681 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 1741 ctgatgttct ggggaatata ataatactag agcccgtaga aaagatcaaa agatcttctt 1801 gagatccttt ttttctgcgc gtaatctgct acttgcaaac aaaaaaacca ccgctaccag 1861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttta 1921 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 1981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtagctactg 2041 ccagtagcga taagtcgtgt cttaccgggt tggattcaag acgatagtta ccggataagg 2101 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2161 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2221 gaaaggcgga caggtatctg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2281 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2341 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2401 cggccttttt acggttcctg gccttttgct ggccttttgc tcacat //