BBa_G00001
1
Suffix
BioBrick cloning site suffix
2006-03-13T12:00:00Z
2015-08-31T04:07:27Z
This part is the standard BioBricks suffix.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1934228
1
NotI
range1934228
1
9
16
annotation1821544
1
Suffix
range1821544
1
1
21
annotation1934226
1
SpeI
range1934226
1
2
7
annotation1934227
1
PstI
range1934227
1
16
21
BBa_G00100
1
VF2
Forward primer for sequencing/amplifying BioBrick parts (VF2)
2004-05-24T11:00:00Z
2015-08-31T04:07:28Z
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
false
false
_11_1_
0
60
7
Not in stock
false
false
Tom Knight
annotation1934505
1
VF2
range1934505
1
1
20
BBa_B0054
1
BBa_B0054
Transcriptional terminator
2005-11-16T12:00:00Z
2015-08-31T04:07:20Z
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
false
false
_41_6_
0
126
44
Not in stock
false
false
Reshma Shetty
annotation1747359
1
stem loop
range1747359
1
11
42
annotation1783342
1
identical to E. coli MG1655 between tonB and yciA
range1783342
1
4
43
BBa_G00000
1
Prefix
BioBrick cloning site prefix
2006-03-13T12:00:00Z
2015-08-31T04:07:27Z
This part is the standard BioBricks prefix.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1934224
1
XbaI site
range1934224
1
16
21
annotation1934225
1
Prefix
range1934225
1
1
22
annotation1934223
1
EcoRI site
range1934223
1
1
6
annotation1961228
1
NotI site
range1961228
1
7
14
BBa_B0044
1
TOPO
Topoisomerase I mediated cloning site (reverse)
2006-02-04T12:00:00Z
2015-08-31T04:07:20Z
Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1797126
1
Nt.BstNB I recognition site
range1797126
1
1
5
annotation1797127
1
Arbitrary sequence (could be any bases)
range1797127
1
6
8
annotation1797129
1
Generated T overhang
range1797129
1
9
9
annotation1797128
1
Topoisomerase I recognition site
range1797128
1
9
13
BBa_B0053
1
BBa_B0053
Terminator (His)
2004-01-29T12:00:00Z
2015-08-31T04:07:20Z
Terminator from the E.coli His operon
false
true
_1_
0
24
7
Not in stock
false
false
Caitlin Conboy
annotation318602
1
stem_loop
range318602
1
9
43
BBa_P1016
1
ccdB
ccdB cassette without ccdA-
2007-01-29T12:00:00Z
2015-06-15T01:13:47Z
This part is derived from BBa_P1010 but it lacks ccdA-.
This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
true
false
_41_
4206
126
70
Not in stock
false
ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
false
Reshma Shetty
annotation1910684
1
-35
range1910684
1
41
46
annotation1910686
1
ccdB
range1910686
1
108
416
annotation1910685
1
-10
range1910685
1
64
69
BBa_G00102
1
VR
Reverse BioBrick primer annealing site (VR binding site)
2006-02-03T12:00:00Z
2015-08-31T04:07:28Z
This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1961229
1
VR
range1961229
1
1
20
BBa_B0043
1
TOPO
Topoisomerase I mediated cloning site (forward)
2006-02-04T12:00:00Z
2015-08-31T04:07:20Z
Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1797103
1
Arbitrary sequence (could be any bases)
range1797103
1
6
8
annotation1797102
1
Nt.BstNB I recognition site
range1797102
1
9
13
annotation1797104
1
Generated T overhang
range1797104
1
5
5
annotation1797101
1
Topoisomerase I recognition site
range1797101
1
1
5
BBa_I51001
1
BBa_I51001
BioBrick base vector, as revised by Codon Devices
2007-01-29T12:00:00Z
2015-08-31T04:07:42Z
This is a composite part generated from several basic parts obtained from a variety of sources.
This is a vector scaffold for constructing new BioBricks vectors. Information on the design is available at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
true
false
_41_
0
126
70
Discontinued
false
Documented at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
Due to technical difficulties during synthesis, rather than including BBa_P1011 as designed, BBa_P1016 was synthesized as a component of this part. Hence, BBa_I51000 documents the original design and BBa_I51001 is the vector scaffold as synthesized.
false
Reshma Shetty
component1910759
1
BBa_I50020
component1910713
1
BBa_B0045
component1910723
1
BBa_G00100
component1910693
1
BBa_G00001
component1910705
1
BBa_B0042
component1910708
1
BBa_B0054
component1910722
1
BBa_B0053
component1910744
1
BBa_P1016
component1910740
1
BBa_G00000
component1910718
1
BBa_P1006
component1910711
1
BBa_B0062
component1910726
1
BBa_B0055
component1910733
1
BBa_B0042
component1910698
1
BBa_B0044
component1910738
1
BBa_B0043
component1910709
1
BBa_G00102
component1910720
1
BBa_B0045
annotation1910744
1
BBa_P1016
range1910744
1
1349
1764
annotation1910726
1
BBa_B0055
range1910726
1
1224
1301
annotation1910698
1
BBa_B0044
range1910698
1
22
34
annotation1910722
1
BBa_B0053
range1910722
1
1132
1203
annotation1910709
1
BBa_G00102
range1910709
1
116
135
annotation1910723
1
BBa_G00100
range1910723
1
1204
1223
annotation1910693
1
BBa_G00001
range1910693
1
1
21
annotation1910733
1
BBa_B0042
range1910733
1
1302
1313
annotation1910711
1
BBa_B0062
range1910711
1
136
176
annotation1910720
1
BBa_B0045
range1910720
1
1126
1131
annotation1910713
1
BBa_B0045
range1910713
1
177
182
annotation1910705
1
BBa_B0042
range1910705
1
35
46
annotation1910740
1
BBa_G00000
range1910740
1
1327
1348
annotation1910738
1
BBa_B0043
range1910738
1
1314
1326
annotation1910718
1
BBa_P1006
range1910718
1
183
1125
annotation1910708
1
BBa_B0054
range1910708
1
47
115
annotation1910759
1
BBa_I50020
range1910759
1
1765
2438
BBa_B0045
1
NheI
NheI restriction enzyme site (XbaI, SpeI compatible overhang)
2006-03-13T12:00:00Z
2015-08-31T04:07:20Z
NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1822724
1
NheI site
range1822724
1
1
6
BBa_B0062
1
BBa_B0062
Terminator (Reverse B0052)
2004-01-29T12:00:00Z
2015-08-31T04:07:21Z
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
false
false
_1_
0
24
7
Not in stock
false
false
Caitlin Conboy
annotation318637
1
stem_loop
range318637
1
14
33
BBa_B0042
1
TSS
Translational stop sequence
2006-02-04T12:00:00Z
2015-08-31T04:07:20Z
This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1797086
1
stop codon (+1 frame)
range1797086
1
10
12
annotation1797088
1
stop codon (-3 frame)
range1797088
1
5
7
annotation1797087
1
stop codon (-2 frame)
range1797087
1
9
11
annotation1797083
1
stop codon (+2 frame)
range1797083
1
2
4
annotation1797085
1
stop codon (+3 frame)
range1797085
1
6
8
annotation1797084
1
stop codon (-1 frame)
range1797084
1
1
3
BBa_P1006
1
ampR
ampicillin resistance cassette, reverse orientation
2006-02-03T12:00:00Z
2015-05-08T01:14:11Z
This contains TetR, a terminator and then AmpR and a terminator
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1897233
1
ampR -10
range1897233
1
882
887
annotation1897231
1
ampicillin resistance
range1897231
1
1
864
annotation1897232
1
ampR RBS
range1897232
1
869
873
annotation1897234
1
ampR -35
range1897234
1
908
913
BBa_B0055
1
BBa_B0055
-- No description --
2005-11-16T12:00:00Z
2015-08-31T04:07:20Z
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
false
false
_41_6_
0
126
44
Not in stock
false
false
Reshma Shetty
annotation1783301
1
stem loop from bacteriophage T3
range1783301
1
36
57
annotation1783311
1
identical to bacteriophage T3
range1783311
1
28
66
BBa_I50020
1
pUC19 ori
Minimal pUC19-derived high copy replication origin
2006-01-09T12:00:00Z
2015-08-31T04:07:41Z
High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites.
false
false
_41_6_
0
126
44
Not in stock
false
false
Reshma Shetty
annotation1910690
1
C to T, nonfunctional mutation
range1910690
1
303
303
annotation1793686
1
RNAII transcript promoter -35 sequence
range1793686
1
27
32
annotation1793681
1
RNAII transcript
range1793681
1
63
615
annotation1910687
1
G to A, nonfunctional mutation
range1910687
1
261
261
annotation1793685
1
RNAII transcript promoter -10 sequence
range1793685
1
48
53
annotation1793680
1
origin of replication
range1793680
1
27
615
annotation1793682
1
RNAI transcript promoter -35
range1793682
1
204
209
annotation1782181
1
ORI
range1782181
1
615
615
annotation1793683
1
RNAI transcript promoter -10
range1793683
1
182
187
annotation1782180
1
rep (pMB1)
range1782180
1
16
630
annotation1910689
1
G to A, nonfunctional mutation
range1910689
1
274
274
annotation1793684
1
RNAI transcript
range1793684
1
66
173
annotation1910691
1
C to T, nonfunctional mutation
range1910691
1
467
467
annotation1910688
1
G to A, nonfunctional mutation
range1910688
1
265
265
BBa_I51001_sequence
1
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttaggctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
BBa_I50020_sequence
1
cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
BBa_B0045_sequence
1
gctagc
BBa_B0062_sequence
1
cagataaaaaaaatccttagctttcgctaaggatgatttct
BBa_P1016_sequence
1
ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
BBa_B0055_sequence
1
aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
BBa_B0044_sequence
1
gagtcactaaggg
BBa_B0042_sequence
1
ttagttagttag
BBa_B0054_sequence
1
attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
BBa_G00000_sequence
1
gaattcgcggccgcttctagag
BBa_G00001_sequence
1
tactagtagcggccgctgcag
BBa_B0053_sequence
1
tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
BBa_G00100_sequence
1
tgccacctgacgtctaagaa
BBa_P1006_sequence
1
ttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttag
BBa_G00102_sequence
1
gctcactcaaaggcggtaat
BBa_B0043_sequence
1
cccttagtgactc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z