BBa_I51001
type: ComponentDefinition
persistent identity: BBa I51001
display id: BBa_I51001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick base vector, as revised by Codon Devices
created: 2007-01-29T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: This is a composite part generated from several basic parts obtained from a variety of sources.
owned by: james
owned by: myers
mutable description: This is a vector scaffold for constructing new BioBricks vectors. Information on the design is available at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
discontinued: true
dominant: false
experience: experience/Issues
group u list: _41_
m user id: 0
owner id: 126
owning group id: 70
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Documented at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design Due to technical difficulties during synthesis, rather than including BBa_P1011 as designed, BBa_P1016 was synthesized as a component of this part. Hence, BBa_I51000 documents the original design and BBa_I51001 is the vector scaffold as synthesized.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
component: BBa_I50020
component: BBa_P1016
component: BBa_G00000
component: BBa_B0043
component: BBa_G00001
component: BBa_G00102
component: BBa_B0054
component: BBa_B0042
component: BBa_B0044
component: BBa_B0045
component: BBa_P1006
component: BBa_B0062
component: BBa_B0055
component: BBa_G00100
component: BBa_B0053
sequence annotation: BBa_G00001
sequence annotation: BBa_B0044
sequence annotation: BBa_B0042
sequence annotation: BBa_B0054
sequence annotation: BBa_G00102
sequence annotation: BBa_B0062
sequence annotation: BBa_B0045
sequence annotation: BBa_P1006
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_B0043
sequence annotation: BBa_G00000
sequence annotation: BBa_P1016
sequence annotation: BBa_I50020
sequence: https://synbiohub.org/public/igem/BBa_I51001_sequence/1

BBa_I50020
type: Component
persistent identity: component1910759
display id: component1910759
version: 1
top level: BBa_I51001
definition: pUC19 ori
access: public

BBa_P1016
type: Component
persistent identity: component1910744
display id: component1910744
version: 1
top level: BBa_I51001
definition: ccdB
access: public

BBa_G00000
type: Component
persistent identity: component1910740
display id: component1910740
version: 1
top level: BBa_I51001
definition: Prefix
access: public

BBa_B0043
type: Component
persistent identity: component1910738
display id: component1910738
version: 1
top level: BBa_I51001
definition: TOPO
access: public

BBa_G00001
type: Component
persistent identity: component1910693
display id: component1910693
version: 1
top level: BBa_I51001
definition: Suffix
access: public

BBa_G00102
type: Component
persistent identity: component1910709
display id: component1910709
version: 1
top level: BBa_I51001
definition: VR
access: public

BBa_B0054
type: Component
persistent identity: component1910708
display id: component1910708
version: 1
top level: BBa_I51001
definition: BBa_B0054
access: public

BBa_B0042
type: Component
persistent identity: component1910705
display id: component1910705
version: 1
top level: BBa_I51001
definition: TSS
access: public

BBa_B0044
type: Component
persistent identity: component1910698
display id: component1910698
version: 1
top level: BBa_I51001
definition: TOPO
access: public

BBa_B0045
type: Component
persistent identity: component1910720
display id: component1910720
version: 1
top level: BBa_I51001
definition: NheI
access: public

BBa_P1006
type: Component
persistent identity: component1910718
display id: component1910718
version: 1
top level: BBa_I51001
definition: ampR
access: public

BBa_B0045
type: Component
persistent identity: component1910713
display id: component1910713
version: 1
top level: BBa_I51001
definition: NheI
access: public

BBa_B0062
type: Component
persistent identity: component1910711
display id: component1910711
version: 1
top level: BBa_I51001
definition: BBa_B0062
access: public

BBa_B0042
type: Component
persistent identity: component1910733
display id: component1910733
version: 1
top level: BBa_I51001
definition: TSS
access: public

BBa_B0055
type: Component
persistent identity: component1910726
display id: component1910726
version: 1
top level: BBa_I51001
definition: BBa_B0055
access: public

BBa_G00100
type: Component
persistent identity: component1910723
display id: component1910723
version: 1
top level: BBa_I51001
definition: VF2
access: public

BBa_B0053
type: Component
persistent identity: component1910722
display id: component1910722
version: 1
top level: BBa_I51001
definition: BBa_B0053
access: public

BBa_G00001
type: SequenceAnnotation
persistent identity: annotation1910693
display id: annotation1910693
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910693/range1910693/1
component: BBa_G00001

https://synbiohub.org/public/igem/BBa_I51001/annotation1910693/range1910693/1
type: Range
persistent identity: range1910693
display id: range1910693
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1
end: 21
orientation: inline

BBa_B0044
type: SequenceAnnotation
persistent identity: annotation1910698
display id: annotation1910698
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910698/range1910698/1
component: BBa_B0044

https://synbiohub.org/public/igem/BBa_I51001/annotation1910698/range1910698/1
type: Range
persistent identity: range1910698
display id: range1910698
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 22
end: 34
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1910705
display id: annotation1910705
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910705/range1910705/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51001/annotation1910705/range1910705/1
type: Range
persistent identity: range1910705
display id: range1910705
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 35
end: 46
orientation: inline

BBa_B0054
type: SequenceAnnotation
persistent identity: annotation1910708
display id: annotation1910708
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910708/range1910708/1
component: BBa_B0054

https://synbiohub.org/public/igem/BBa_I51001/annotation1910708/range1910708/1
type: Range
persistent identity: range1910708
display id: range1910708
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 47
end: 115
orientation: inline

BBa_G00102
type: SequenceAnnotation
persistent identity: annotation1910709
display id: annotation1910709
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910709/range1910709/1
component: BBa_G00102

https://synbiohub.org/public/igem/BBa_I51001/annotation1910709/range1910709/1
type: Range
persistent identity: range1910709
display id: range1910709
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 116
end: 135
orientation: inline

BBa_B0062
type: SequenceAnnotation
persistent identity: annotation1910711
display id: annotation1910711
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910711/range1910711/1
component: BBa_B0062

https://synbiohub.org/public/igem/BBa_I51001/annotation1910711/range1910711/1
type: Range
persistent identity: range1910711
display id: range1910711
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 136
end: 176
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1910713
display id: annotation1910713
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910713/range1910713/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51001/annotation1910713/range1910713/1
type: Range
persistent identity: range1910713
display id: range1910713
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 177
end: 182
orientation: inline

BBa_P1006
type: SequenceAnnotation
persistent identity: annotation1910718
display id: annotation1910718
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910718/range1910718/1
component: BBa_P1006

https://synbiohub.org/public/igem/BBa_I51001/annotation1910718/range1910718/1
type: Range
persistent identity: range1910718
display id: range1910718
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 183
end: 1125
orientation: inline

BBa_B0045
type: SequenceAnnotation
persistent identity: annotation1910720
display id: annotation1910720
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910720/range1910720/1
component: BBa_B0045

https://synbiohub.org/public/igem/BBa_I51001/annotation1910720/range1910720/1
type: Range
persistent identity: range1910720
display id: range1910720
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1126
end: 1131
orientation: inline

BBa_B0053
type: SequenceAnnotation
persistent identity: annotation1910722
display id: annotation1910722
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910722/range1910722/1
component: BBa_B0053

https://synbiohub.org/public/igem/BBa_I51001/annotation1910722/range1910722/1
type: Range
persistent identity: range1910722
display id: range1910722
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1132
end: 1203
orientation: inline

BBa_G00100
type: SequenceAnnotation
persistent identity: annotation1910723
display id: annotation1910723
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910723/range1910723/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51001/annotation1910723/range1910723/1
type: Range
persistent identity: range1910723
display id: range1910723
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1204
end: 1223
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1910726
display id: annotation1910726
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910726/range1910726/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51001/annotation1910726/range1910726/1
type: Range
persistent identity: range1910726
display id: range1910726
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1224
end: 1301
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1910733
display id: annotation1910733
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910733/range1910733/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51001/annotation1910733/range1910733/1
type: Range
persistent identity: range1910733
display id: range1910733
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1302
end: 1313
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1910738
display id: annotation1910738
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910738/range1910738/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51001/annotation1910738/range1910738/1
type: Range
persistent identity: range1910738
display id: range1910738
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1314
end: 1326
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1910740
display id: annotation1910740
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910740/range1910740/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51001/annotation1910740/range1910740/1
type: Range
persistent identity: range1910740
display id: range1910740
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1327
end: 1348
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1910744
display id: annotation1910744
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910744/range1910744/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51001/annotation1910744/range1910744/1
type: Range
persistent identity: range1910744
display id: range1910744
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1349
end: 1764
orientation: inline

BBa_I50020
type: SequenceAnnotation
persistent identity: annotation1910759
display id: annotation1910759
version: 1
top level: BBa_I51001
location: https://synbiohub.org/public/igem/BBa_I51001/annotation1910759/range1910759/1
component: BBa_I50020

https://synbiohub.org/public/igem/BBa_I51001/annotation1910759/range1910759/1
type: Range
persistent identity: range1910759
display id: range1910759
version: 1
top level: BBa_I51001
direction: direction/reverse
start: 1765
end: 2438
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0044
display id: BBa_B0044
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (reverse)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Nt.BstNB I recognition site
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence: https://synbiohub.org/public/igem/BBa_B0044_sequence/1

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797126
display id: annotation1797126
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
type: Range
persistent identity: range1797126
display id: range1797126
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797127
display id: annotation1797127
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
type: Range
persistent identity: range1797127
display id: range1797127
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797128
display id: annotation1797128
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
type: Range
persistent identity: range1797128
display id: range1797128
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797129
display id: annotation1797129
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
type: Range
persistent identity: range1797129
display id: range1797129
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 9
orientation: inline

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Suffix
sequence annotation: SpeI
sequence annotation: NotI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1016
display id: BBa_P1016
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cassette without ccdA-
created: 2007-01-29T12:00:00Z
modified: 2015-06-15T01:13:47Z
mutable provenance: This part is derived from BBa_P1010 but it lacks ccdA-.
owned by: james
owned by: myers
mutable description: This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 4206
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: -35
sequence annotation: -10
sequence annotation: ccdB
sequence: https://synbiohub.org/public/igem/BBa_P1016_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1910684
display id: annotation1910684
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
type: Range
persistent identity: range1910684
display id: range1910684
version: 1
top level: ccdB
direction: direction/forward
start: 41
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1910685
display id: annotation1910685
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
type: Range
persistent identity: range1910685
display id: range1910685
version: 1
top level: ccdB
direction: direction/forward
start: 64
end: 69
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1910686
display id: annotation1910686
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
type: Range
persistent identity: range1910686
display id: range1910686
version: 1
top level: ccdB
direction: direction/forward
start: 108
end: 416
orientation: inline

TSS
type: ComponentDefinition
persistent identity: BBa B0042
display id: BBa_B0042
version: 1
was generated by: iGEM to SBOL conversion
description: Translational stop sequence
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: stop codon (-1 frame)
sequence annotation: stop codon (+2 frame)
sequence annotation: stop codon (-3 frame)
sequence annotation: stop codon (+3 frame)
sequence annotation: stop codon (-2 frame)
sequence annotation: stop codon (+1 frame)
sequence: https://synbiohub.org/public/igem/BBa_B0042_sequence/1

stop codon (-1 frame)
type: SequenceAnnotation
persistent identity: annotation1797084
display id: annotation1797084
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
type: Range
persistent identity: range1797084
display id: range1797084
version: 1
top level: TSS
direction: direction/reverse
start: 1
end: 3
orientation: inline

stop codon (+2 frame)
type: SequenceAnnotation
persistent identity: annotation1797083
display id: annotation1797083
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
type: Range
persistent identity: range1797083
display id: range1797083
version: 1
top level: TSS
direction: direction/forward
start: 2
end: 4
orientation: inline

stop codon (-3 frame)
type: SequenceAnnotation
persistent identity: annotation1797088
display id: annotation1797088
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
type: Range
persistent identity: range1797088
display id: range1797088
version: 1
top level: TSS
direction: direction/reverse
start: 5
end: 7
orientation: inline

stop codon (+3 frame)
type: SequenceAnnotation
persistent identity: annotation1797085
display id: annotation1797085
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
type: Range
persistent identity: range1797085
display id: range1797085
version: 1
top level: TSS
direction: direction/forward
start: 6
end: 8
orientation: inline

stop codon (-2 frame)
type: SequenceAnnotation
persistent identity: annotation1797087
display id: annotation1797087
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
type: Range
persistent identity: range1797087
display id: range1797087
version: 1
top level: TSS
direction: direction/reverse
start: 9
end: 11
orientation: inline

stop codon (+1 frame)
type: SequenceAnnotation
persistent identity: annotation1797086
display id: annotation1797086
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
type: Range
persistent identity: range1797086
display id: range1797086
version: 1
top level: TSS
direction: direction/forward
start: 10
end: 12
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0043
display id: BBa_B0043
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (forward)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Nt.BstNB I recognition site
sequence: https://synbiohub.org/public/igem/BBa_B0043_sequence/1

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797101
display id: annotation1797101
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
type: Range
persistent identity: range1797101
display id: range1797101
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797104
display id: annotation1797104
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
type: Range
persistent identity: range1797104
display id: range1797104
version: 1
top level: TOPO
direction: direction/forward
start: 5
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797103
display id: annotation1797103
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
type: Range
persistent identity: range1797103
display id: range1797103
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797102
display id: annotation1797102
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
type: Range
persistent identity: range1797102
display id: range1797102
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Prefix
sequence annotation: EcoRI site
sequence annotation: NotI site
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa P1006
display id: BBa_P1006
version: 1
was generated by: iGEM to SBOL conversion
description: ampicillin resistance cassette, reverse orientation
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: This contains TetR, a terminator and then AmpR and a terminator
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: ampicillin resistance
sequence annotation: ampR RBS
sequence annotation: ampR -10
sequence annotation: ampR -35
sequence: https://synbiohub.org/public/igem/BBa_P1006_sequence/1

ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation1897231
display id: annotation1897231
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897231/range1897231/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1006/annotation1897231/range1897231/1
type: Range
persistent identity: range1897231
display id: range1897231
version: 1
top level: ampR
direction: direction/reverse
start: 1
end: 864
orientation: inline

ampR RBS
type: SequenceAnnotation
persistent identity: annotation1897232
display id: annotation1897232
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897232/range1897232/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_P1006/annotation1897232/range1897232/1
type: Range
persistent identity: range1897232
display id: range1897232
version: 1
top level: ampR
direction: direction/reverse
start: 869
end: 873
orientation: inline

ampR -10
type: SequenceAnnotation
persistent identity: annotation1897233
display id: annotation1897233
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897233/range1897233/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1006/annotation1897233/range1897233/1
type: Range
persistent identity: range1897233
display id: range1897233
version: 1
top level: ampR
direction: direction/reverse
start: 882
end: 887
orientation: inline

ampR -35
type: SequenceAnnotation
persistent identity: annotation1897234
display id: annotation1897234
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897234/range1897234/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1006/annotation1897234/range1897234/1
type: Range
persistent identity: range1897234
display id: range1897234
version: 1
top level: ampR
direction: direction/reverse
start: 908
end: 913
orientation: inline

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50020
display id: BBa_I50020
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal pUC19-derived high copy replication origin
created: 2006-01-09T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: rep (pMB1)
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: origin of replication
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: RNAI transcript
sequence annotation: RNAI transcript promoter -10
sequence annotation: RNAI transcript promoter -35
sequence annotation: G to A, nonfunctional mutation
sequence annotation: C to T, nonfunctional mutation
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_I50020_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1782180
display id: annotation1782180
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1782180/range1782180/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50020/annotation1782180/range1782180/1
type: Range
persistent identity: range1782180
display id: range1782180
version: 1
top level: pUC19 ori
direction: direction/forward
start: 16
end: 630
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1793686
display id: annotation1793686
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793686/range1793686/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793686/range1793686/1
type: Range
persistent identity: range1793686
display id: range1793686
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 32
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1793680
display id: annotation1793680
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793680/range1793680/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1793680/range1793680/1
type: Range
persistent identity: range1793680
display id: range1793680
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 615
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1793685
display id: annotation1793685
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793685/range1793685/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793685/range1793685/1
type: Range
persistent identity: range1793685
display id: range1793685
version: 1
top level: pUC19 ori
direction: direction/forward
start: 48
end: 53
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1793681
display id: annotation1793681
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793681/range1793681/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50020/annotation1793681/range1793681/1
type: Range
persistent identity: range1793681
display id: range1793681
version: 1
top level: pUC19 ori
direction: direction/forward
start: 63
end: 615
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1793684
display id: annotation1793684
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793684/range1793684/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1793684/range1793684/1
type: Range
persistent identity: range1793684
display id: range1793684
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 66
end: 173
orientation: inline

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1793683
display id: annotation1793683
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793683/range1793683/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793683/range1793683/1
type: Range
persistent identity: range1793683
display id: range1793683
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 182
end: 187
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1793682
display id: annotation1793682
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793682/range1793682/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793682/range1793682/1
type: Range
persistent identity: range1793682
display id: range1793682
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 204
end: 209
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910687
display id: annotation1910687
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910687/range1910687/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910687/range1910687/1
type: Range
persistent identity: range1910687
display id: range1910687
version: 1
top level: pUC19 ori
direction: direction/forward
start: 261
end: 261
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910688
display id: annotation1910688
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910688/range1910688/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910688/range1910688/1
type: Range
persistent identity: range1910688
display id: range1910688
version: 1
top level: pUC19 ori
direction: direction/forward
start: 265
end: 265
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910689
display id: annotation1910689
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910689/range1910689/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910689/range1910689/1
type: Range
persistent identity: range1910689
display id: range1910689
version: 1
top level: pUC19 ori
direction: direction/forward
start: 274
end: 274
orientation: inline

C to T, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910690
display id: annotation1910690
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910690/range1910690/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910690/range1910690/1
type: Range
persistent identity: range1910690
display id: range1910690
version: 1
top level: pUC19 ori
direction: direction/forward
start: 303
end: 303
orientation: inline

C to T, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910691
display id: annotation1910691
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910691/range1910691/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910691/range1910691/1
type: Range
persistent identity: range1910691
display id: range1910691
version: 1
top level: pUC19 ori
direction: direction/forward
start: 467
end: 467
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1782181
display id: annotation1782181
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1782181/range1782181/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1782181/range1782181/1
type: Range
persistent identity: range1782181
display id: range1782181
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 615
end: 615
orientation: inline

https://synbiohub.org/public/igem/BBa_I51001_sequence/1
type: Sequence
persistent identity: BBa I51001 sequence
display id: BBa_I51001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttaggctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0044_sequence/1
type: Sequence
persistent identity: BBa B0044 sequence
display id: BBa_B0044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtcactaaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1016_sequence/1
type: Sequence
persistent identity: BBa P1016 sequence
display id: BBa_P1016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0042_sequence/1
type: Sequence
persistent identity: BBa B0042 sequence
display id: BBa_B0042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagttagttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0043_sequence/1
type: Sequence
persistent identity: BBa B0043 sequence
display id: BBa_B0043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttagtgactc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1006_sequence/1
type: Sequence
persistent identity: BBa P1006 sequence
display id: BBa_P1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50020_sequence/1
type: Sequence
persistent identity: BBa I50020 sequence
display id: BBa_I50020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z