BBa_G00102
1
VR
Reverse BioBrick primer annealing site (VR binding site)
2006-02-03T12:00:00Z
2015-08-31T04:07:28Z
This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1961229
1
VR
range1961229
1
1
20
BBa_P1006
1
ampR
ampicillin resistance cassette, reverse orientation
2006-02-03T12:00:00Z
2015-05-08T01:14:11Z
This contains TetR, a terminator and then AmpR and a terminator
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1897233
1
ampR -10
range1897233
1
882
887
annotation1897232
1
ampR RBS
range1897232
1
869
873
annotation1897231
1
ampicillin resistance
range1897231
1
1
864
annotation1897234
1
ampR -35
range1897234
1
908
913
BBa_P1016
1
ccdB
ccdB cassette without ccdA-
2007-01-29T12:00:00Z
2015-06-15T01:13:47Z
This part is derived from BBa_P1010 but it lacks ccdA-.
This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
true
false
_41_
4206
126
70
Not in stock
false
ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
false
Reshma Shetty
annotation1910684
1
-35
range1910684
1
41
46
annotation1910685
1
-10
range1910685
1
64
69
annotation1910686
1
ccdB
range1910686
1
108
416
BBa_I50020
1
pUC19 ori
Minimal pUC19-derived high copy replication origin
2006-01-09T12:00:00Z
2015-08-31T04:07:41Z
High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites.
false
false
_41_6_
0
126
44
Not in stock
false
false
Reshma Shetty
annotation1793682
1
RNAI transcript promoter -35
range1793682
1
204
209
annotation1793683
1
RNAI transcript promoter -10
range1793683
1
182
187
annotation1793680
1
origin of replication
range1793680
1
27
615
annotation1782180
1
rep (pMB1)
range1782180
1
16
630
annotation1782181
1
ORI
range1782181
1
615
615
annotation1910690
1
C to T, nonfunctional mutation
range1910690
1
303
303
annotation1910689
1
G to A, nonfunctional mutation
range1910689
1
274
274
annotation1793684
1
RNAI transcript
range1793684
1
66
173
annotation1910691
1
C to T, nonfunctional mutation
range1910691
1
467
467
annotation1910687
1
G to A, nonfunctional mutation
range1910687
1
261
261
annotation1793686
1
RNAII transcript promoter -35 sequence
range1793686
1
27
32
annotation1793685
1
RNAII transcript promoter -10 sequence
range1793685
1
48
53
annotation1793681
1
RNAII transcript
range1793681
1
63
615
annotation1910688
1
G to A, nonfunctional mutation
range1910688
1
265
265
BBa_B0053
1
BBa_B0053
Terminator (His)
2004-01-29T12:00:00Z
2015-08-31T04:07:20Z
Terminator from the E.coli His operon
false
true
_1_
0
24
7
Not in stock
false
false
Caitlin Conboy
annotation318602
1
stem_loop
range318602
1
9
43
BBa_B0043
1
TOPO
Topoisomerase I mediated cloning site (forward)
2006-02-04T12:00:00Z
2015-08-31T04:07:20Z
Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1797101
1
Topoisomerase I recognition site
range1797101
1
1
5
annotation1797102
1
Nt.BstNB I recognition site
range1797102
1
9
13
annotation1797103
1
Arbitrary sequence (could be any bases)
range1797103
1
6
8
annotation1797104
1
Generated T overhang
range1797104
1
5
5
BBa_B0042
1
TSS
Translational stop sequence
2006-02-04T12:00:00Z
2015-08-31T04:07:20Z
This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1797084
1
stop codon (-1 frame)
range1797084
1
1
3
annotation1797086
1
stop codon (+1 frame)
range1797086
1
10
12
annotation1797083
1
stop codon (+2 frame)
range1797083
1
2
4
annotation1797085
1
stop codon (+3 frame)
range1797085
1
6
8
annotation1797087
1
stop codon (-2 frame)
range1797087
1
9
11
annotation1797088
1
stop codon (-3 frame)
range1797088
1
5
7
BBa_B0062
1
BBa_B0062
Terminator (Reverse B0052)
2004-01-29T12:00:00Z
2015-08-31T04:07:21Z
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
false
false
_1_
0
24
7
Not in stock
false
false
Caitlin Conboy
annotation318637
1
stem_loop
range318637
1
14
33
BBa_I51019
1
BBa_I51019
BioBrick base vector, as synthesized by Codon Devices
2008-03-22T12:00:00Z
2015-08-31T04:07:42Z
[[Part:BBa_I51001|BBa_I51001]]
[[Part:BBa_I51019|BBa_I51019]] is the sequence of the BioBrick base vector as synthesized by Codon Devices, Inc. in Cambridge, MA. The original design of the BioBrick base vector is available as [[Part:BBa_I51000|BBa_I51000]]. [[Part:BBa_I51001|BBa_I51001]] is the sequence of the BioBrick base vector as reported by Codon Devices, Inc. although the true sequence is something like to [[Part:BBa_I51019|BBa_I51019]]. The exact sequence of the provided DNA is difficult to determine. The corrected BioBrick base vector that was successfully constructed from [[Part:BBa_I51019|BBa_I51019]] is [[Part:BBa_I51020|BBa_I51020]].
true
false
_41_
0
126
162
Discontinued
false
Synthesis of the BioBrick base vector design ([[Part:BBa_I51000|BBa_I51000]]) was problematic. We encountered two issues while trying to construct the base vector. First, troubleshooting efforts during synthesis compromised the design of our parts: failed attempts to clone the base vector into an ''E. coli'' strain intolerant of expression of the toxic protein CcdB led to an unnecessary redesign of the ''ccdB'' positive selection marker in the BioBrick base vector. As a result, a revised design for the BioBrick base vector ([[Part:BBa_I51001|BBa_I51001]]) was specified instead. Second, faulty part design adversely impacted the synthesis process: our pUC19-based replication origin design ([[Part:BBa_I50020|BBa_I50020]]) was similarly nonfunctional, so the revised base vector ([[Part:BBa_I51001|BBa_I51001]]) could not be propagated as specified. We eventually determined that the provided DNA for the BioBrick base vector was actually a fusion of two slightly different copies of the base vector: one with a nonfunctional version of the pUC19 origin ([[Part:BBa_I50020|BBa_I50020]]) and one with a functional version of the pUC19 origin ([[Part:BBa_I50022|BBa_I50022]]). We corrected the synthesized BioBrick base vector ([[Part:BBa_I51019|BBa_I51019]]) with molecular biology techniques to make the functional BioBrick base vector [[Part:BBa_I51020|BBa_I51020]] (see Methods). Commercial DNA synthesis processes currently rely on cloning, assembly and propagation of synthesized DNA in ''E. coli''. Difficulties during synthesis stemmed from the inclusion of both a ''ccdB'' positive selection marker that is toxic to most ''E. coli'' strains and a synthetic origin incapable of supporting plasmid replication of the BioBrick base vector. In general, for parts whose function are incompatible with growth and replication of ''E. coli'', the processes of DNA design and DNA synthesis cannot be easily decoupled.
false
Reshma Shetty
component1961871
1
BBa_B0054
component1961868
1
BBa_B0042
component1961888
1
BBa_G00100
component1961875
1
BBa_B0062
component1961882
1
BBa_P1006
component1961998
1
BBa_I50022
component1961908
1
BBa_G00000
component1961898
1
BBa_B0042
component1961974
1
BBa_B0042
component1961967
1
BBa_B0055
component1961856
1
BBa_G00001
component1961951
1
BBa_B0062
component1961861
1
BBa_B0044
component1961884
1
BBa_B0045
component1961912
1
BBa_P1016
component1961988
1
BBa_P1016
component1961927
1
BBa_I50020
component1961891
1
BBa_B0055
component1961873
1
BBa_G00102
component1961960
1
BBa_B0045
component1961944
1
BBa_B0042
component1961958
1
BBa_P1006
component1961953
1
BBa_B0045
component1961964
1
BBa_G00100
component1961886
1
BBa_B0053
component1961949
1
BBa_G00102
component1961979
1
BBa_B0043
component1961877
1
BBa_B0045
component1961947
1
BBa_B0054
component1961962
1
BBa_B0053
component1961903
1
BBa_B0043
component1961937
1
BBa_B0044
component1961984
1
BBa_G00000
component1961932
1
BBa_G00001
annotation1961949
1
BBa_G00102
range1961949
1
2554
2573
annotation1961898
1
BBa_B0042
range1961898
1
1302
1313
annotation1961974
1
BBa_B0042
range1961974
1
3740
3751
annotation1961908
1
BBa_G00000
range1961908
1
1327
1348
annotation1961927
1
BBa_I50020
range1961927
1
1765
2438
annotation1961871
1
BBa_B0054
range1961871
1
47
115
annotation1961856
1
BBa_G00001
range1961856
1
1
21
annotation1961873
1
BBa_G00102
range1961873
1
116
135
annotation1961953
1
BBa_B0045
range1961953
1
2615
2620
annotation1961932
1
BBa_G00001
range1961932
1
2439
2459
annotation1961891
1
BBa_B0055
range1961891
1
1224
1301
annotation1961998
1
BBa_I50022
range1961998
1
4203
4876
annotation1961868
1
BBa_B0042
range1961868
1
35
46
annotation1961958
1
BBa_P1006
range1961958
1
2621
3563
annotation1961944
1
BBa_B0042
range1961944
1
2473
2484
annotation1961875
1
BBa_B0062
range1961875
1
136
176
annotation1961861
1
BBa_B0044
range1961861
1
22
34
annotation1961884
1
BBa_B0045
range1961884
1
1126
1131
annotation1961984
1
BBa_G00000
range1961984
1
3765
3786
annotation1961947
1
BBa_B0054
range1961947
1
2485
2553
annotation1961960
1
BBa_B0045
range1961960
1
3564
3569
annotation1961882
1
BBa_P1006
range1961882
1
183
1125
annotation1961886
1
BBa_B0053
range1961886
1
1132
1203
annotation1961962
1
BBa_B0053
range1961962
1
3570
3641
annotation1961888
1
BBa_G00100
range1961888
1
1204
1223
annotation1961951
1
BBa_B0062
range1961951
1
2574
2614
annotation1961937
1
BBa_B0044
range1961937
1
2460
2472
annotation1961988
1
BBa_P1016
range1961988
1
3787
4202
annotation1961912
1
BBa_P1016
range1961912
1
1349
1764
annotation1961903
1
BBa_B0043
range1961903
1
1314
1326
annotation1961877
1
BBa_B0045
range1961877
1
177
182
annotation1961979
1
BBa_B0043
range1961979
1
3752
3764
annotation1961967
1
BBa_B0055
range1961967
1
3662
3739
annotation1961964
1
BBa_G00100
range1961964
1
3642
3661
BBa_I50022
1
pUC19 ori
Minimal pUC19-derived high copy replication origin
2008-01-05T12:00:00Z
2015-08-31T04:07:42Z
pSB1A3
High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.
false
false
_41_
0
126
162
Not in stock
false
We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.
false
Reshma Shetty
annotation1959117
1
rep (pMB1)
range1959117
1
16
630
annotation1959124
1
RNAI transcript promoter -35
range1959124
1
204
209
annotation1959119
1
origin of replication
range1959119
1
27
615
annotation1959120
1
RNAII transcript promoter -10 sequence
range1959120
1
48
53
annotation1959122
1
RNAI transcript
range1959122
1
66
173
annotation1959125
1
ORI
range1959125
1
615
615
annotation1959121
1
RNAII transcript
range1959121
1
63
615
annotation1959118
1
RNAII transcript promoter -35 sequence
range1959118
1
27
32
annotation1959123
1
RNAI transcript promoter -10
range1959123
1
182
187
BBa_G00001
1
Suffix
BioBrick cloning site suffix
2006-03-13T12:00:00Z
2015-08-31T04:07:27Z
This part is the standard BioBricks suffix.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1934228
1
NotI
range1934228
1
9
16
annotation1934226
1
SpeI
range1934226
1
2
7
annotation1821544
1
Suffix
range1821544
1
1
21
annotation1934227
1
PstI
range1934227
1
16
21
BBa_B0055
1
BBa_B0055
-- No description --
2005-11-16T12:00:00Z
2015-08-31T04:07:20Z
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
false
false
_41_6_
0
126
44
Not in stock
false
false
Reshma Shetty
annotation1783311
1
identical to bacteriophage T3
range1783311
1
28
66
annotation1783301
1
stem loop from bacteriophage T3
range1783301
1
36
57
BBa_B0054
1
BBa_B0054
Transcriptional terminator
2005-11-16T12:00:00Z
2015-08-31T04:07:20Z
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
false
false
_41_6_
0
126
44
Not in stock
false
false
Reshma Shetty
annotation1747359
1
stem loop
range1747359
1
11
42
annotation1783342
1
identical to E. coli MG1655 between tonB and yciA
range1783342
1
4
43
BBa_B0044
1
TOPO
Topoisomerase I mediated cloning site (reverse)
2006-02-04T12:00:00Z
2015-08-31T04:07:20Z
Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
false
false
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1797129
1
Generated T overhang
range1797129
1
9
9
annotation1797128
1
Topoisomerase I recognition site
range1797128
1
9
13
annotation1797126
1
Nt.BstNB I recognition site
range1797126
1
1
5
annotation1797127
1
Arbitrary sequence (could be any bases)
range1797127
1
6
8
BBa_B0045
1
NheI
NheI restriction enzyme site (XbaI, SpeI compatible overhang)
2006-03-13T12:00:00Z
2015-08-31T04:07:20Z
NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1822724
1
NheI site
range1822724
1
1
6
BBa_G00100
1
VF2
Forward primer for sequencing/amplifying BioBrick parts (VF2)
2004-05-24T11:00:00Z
2015-08-31T04:07:28Z
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
false
false
_11_1_
0
60
7
Not in stock
false
false
Tom Knight
annotation1934505
1
VF2
range1934505
1
1
20
BBa_G00000
1
Prefix
BioBrick cloning site prefix
2006-03-13T12:00:00Z
2015-08-31T04:07:27Z
This part is the standard BioBricks prefix.
false
true
_41_6_
0
126
45
Not in stock
false
false
Reshma Shetty
annotation1934225
1
Prefix
range1934225
1
1
22
annotation1961228
1
NotI site
range1961228
1
7
14
annotation1934223
1
EcoRI site
range1934223
1
1
6
annotation1934224
1
XbaI site
range1934224
1
16
21
BBa_I50022_sequence
1
cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
BBa_I51019_sequence
1
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttaggctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacattactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttaggctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
BBa_I50020_sequence
1
cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
BBa_B0045_sequence
1
gctagc
BBa_B0062_sequence
1
cagataaaaaaaatccttagctttcgctaaggatgatttct
BBa_P1016_sequence
1
ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
BBa_B0055_sequence
1
aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
BBa_B0044_sequence
1
gagtcactaaggg
BBa_B0042_sequence
1
ttagttagttag
BBa_B0054_sequence
1
attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
BBa_G00000_sequence
1
gaattcgcggccgcttctagag
BBa_G00001_sequence
1
tactagtagcggccgctgcag
BBa_B0053_sequence
1
tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
BBa_G00100_sequence
1
tgccacctgacgtctaagaa
BBa_P1006_sequence
1
ttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttag
BBa_G00102_sequence
1
gctcactcaaaggcggtaat
BBa_B0043_sequence
1
cccttagtgactc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z