BBa_I51019
type: ComponentDefinition
persistent identity: BBa I51019
display id: BBa_I51019
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick base vector, as synthesized by Codon Devices
created: 2008-03-22T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: BBa_I51001
owned by: james
owned by: myers
mutable description: BBa_I51019 is the sequence of the BioBrick base vector as synthesized by Codon Devices, Inc. in Cambridge, MA. The original design of the BioBrick base vector is available as BBa_I51000. BBa_I51001 is the sequence of the BioBrick base vector as reported by Codon Devices, Inc. although the true sequence is something like to BBa_I51019. The exact sequence of the provided DNA is difficult to determine. The corrected BioBrick base vector that was successfully constructed from BBa_I51019 is BBa_I51020.
discontinued: true
dominant: false
experience: experience/None
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: Synthesis of the BioBrick base vector design (BBa_I51000) was problematic. We encountered two issues while trying to construct the base vector. First, troubleshooting efforts during synthesis compromised the design of our parts: failed attempts to clone the base vector into an ''E. coli'' strain intolerant of expression of the toxic protein CcdB led to an unnecessary redesign of the ''ccdB'' positive selection marker in the BioBrick base vector. As a result, a revised design for the BioBrick base vector (BBa_I51001) was specified instead. Second, faulty part design adversely impacted the synthesis process: our pUC19-based replication origin design (BBa_I50020) was similarly nonfunctional, so the revised base vector (BBa_I51001) could not be propagated as specified. We eventually determined that the provided DNA for the BioBrick base vector was actually a fusion of two slightly different copies of the base vector: one with a nonfunctional version of the pUC19 origin (BBa_I50020) and one with a functional version of the pUC19 origin (BBa_I50022). We corrected the synthesized BioBrick base vector (BBa_I51019) with molecular biology techniques to make the functional BioBrick base vector BBa_I51020 (see Methods). Commercial DNA synthesis processes currently rely on cloning, assembly and propagation of synthesized DNA in ''E. coli''. Difficulties during synthesis stemmed from the inclusion of both a ''ccdB'' positive selection marker that is toxic to most ''E. coli'' strains and a synthetic origin incapable of supporting plasmid replication of the BioBrick base vector. In general, for parts whose function are incompatible with growth and replication of ''E. coli'', the processes of DNA design and DNA synthesis cannot be easily decoupled.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
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component: BBa_B0045
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component: BBa_B0055
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component: BBa_B0043
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component: BBa_G00000
component: BBa_I50022
component: BBa_I50020
component: BBa_B0044
component: BBa_G00001
component: BBa_B0054
component: BBa_B0062
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sequence annotation: BBa_B0044
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sequence annotation: BBa_P1006
sequence annotation: BBa_B0053
sequence annotation: BBa_G00100
sequence annotation: BBa_B0055
sequence annotation: BBa_B0043
sequence annotation: BBa_G00000
sequence annotation: BBa_P1016
sequence annotation: BBa_I50020

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top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961964/range1961964/1
component: BBa_G00100

https://synbiohub.org/public/igem/BBa_I51019/annotation1961964/range1961964/1
type: Range
persistent identity: range1961964
display id: range1961964
version: 1
top level: BBa_I51019
direction: direction/reverse
start: 3642
end: 3661
orientation: inline

BBa_B0055
type: SequenceAnnotation
persistent identity: annotation1961967
display id: annotation1961967
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961967/range1961967/1
component: BBa_B0055

https://synbiohub.org/public/igem/BBa_I51019/annotation1961967/range1961967/1
type: Range
persistent identity: range1961967
display id: range1961967
version: 1
top level: BBa_I51019
direction: direction/reverse
start: 3662
end: 3739
orientation: inline

BBa_B0042
type: SequenceAnnotation
persistent identity: annotation1961974
display id: annotation1961974
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961974/range1961974/1
component: BBa_B0042

https://synbiohub.org/public/igem/BBa_I51019/annotation1961974/range1961974/1
type: Range
persistent identity: range1961974
display id: range1961974
version: 1
top level: BBa_I51019
direction: direction/reverse
start: 3740
end: 3751
orientation: inline

BBa_B0043
type: SequenceAnnotation
persistent identity: annotation1961979
display id: annotation1961979
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961979/range1961979/1
component: BBa_B0043

https://synbiohub.org/public/igem/BBa_I51019/annotation1961979/range1961979/1
type: Range
persistent identity: range1961979
display id: range1961979
version: 1
top level: BBa_I51019
direction: direction/reverse
start: 3752
end: 3764
orientation: inline

BBa_G00000
type: SequenceAnnotation
persistent identity: annotation1961984
display id: annotation1961984
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961984/range1961984/1
component: BBa_G00000

https://synbiohub.org/public/igem/BBa_I51019/annotation1961984/range1961984/1
type: Range
persistent identity: range1961984
display id: range1961984
version: 1
top level: BBa_I51019
direction: direction/reverse
start: 3765
end: 3786
orientation: inline

BBa_P1016
type: SequenceAnnotation
persistent identity: annotation1961988
display id: annotation1961988
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961988/range1961988/1
component: BBa_P1016

https://synbiohub.org/public/igem/BBa_I51019/annotation1961988/range1961988/1
type: Range
persistent identity: range1961988
display id: range1961988
version: 1
top level: BBa_I51019
direction: direction/reverse
start: 3787
end: 4202
orientation: inline

BBa_I50022
type: SequenceAnnotation
persistent identity: annotation1961998
display id: annotation1961998
version: 1
top level: BBa_I51019
location: https://synbiohub.org/public/igem/BBa_I51019/annotation1961998/range1961998/1
component: BBa_I50022

https://synbiohub.org/public/igem/BBa_I51019/annotation1961998/range1961998/1
type: Range
persistent identity: range1961998
display id: range1961998
version: 1
top level: BBa_I51019
direction: direction/reverse
start: 4203
end: 4876
orientation: inline

TSS
type: ComponentDefinition
persistent identity: BBa B0042
display id: BBa_B0042
version: 1
was generated by: iGEM to SBOL conversion
description: Translational stop sequence
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: stop codon (-1 frame)
sequence annotation: stop codon (+2 frame)
sequence annotation: stop codon (-3 frame)
sequence annotation: stop codon (+3 frame)
sequence annotation: stop codon (-2 frame)
sequence annotation: stop codon (+1 frame)
sequence: https://synbiohub.org/public/igem/BBa_B0042_sequence/1

stop codon (-1 frame)
type: SequenceAnnotation
persistent identity: annotation1797084
display id: annotation1797084
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797084/range1797084/1
type: Range
persistent identity: range1797084
display id: range1797084
version: 1
top level: TSS
direction: direction/reverse
start: 1
end: 3
orientation: inline

stop codon (+2 frame)
type: SequenceAnnotation
persistent identity: annotation1797083
display id: annotation1797083
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797083/range1797083/1
type: Range
persistent identity: range1797083
display id: range1797083
version: 1
top level: TSS
direction: direction/forward
start: 2
end: 4
orientation: inline

stop codon (-3 frame)
type: SequenceAnnotation
persistent identity: annotation1797088
display id: annotation1797088
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797088/range1797088/1
type: Range
persistent identity: range1797088
display id: range1797088
version: 1
top level: TSS
direction: direction/reverse
start: 5
end: 7
orientation: inline

stop codon (+3 frame)
type: SequenceAnnotation
persistent identity: annotation1797085
display id: annotation1797085
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797085/range1797085/1
type: Range
persistent identity: range1797085
display id: range1797085
version: 1
top level: TSS
direction: direction/forward
start: 6
end: 8
orientation: inline

stop codon (-2 frame)
type: SequenceAnnotation
persistent identity: annotation1797087
display id: annotation1797087
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0042/annotation1797087/range1797087/1
type: Range
persistent identity: range1797087
display id: range1797087
version: 1
top level: TSS
direction: direction/reverse
start: 9
end: 11
orientation: inline

stop codon (+1 frame)
type: SequenceAnnotation
persistent identity: annotation1797086
display id: annotation1797086
version: 1
top level: TSS
location: https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0042/annotation1797086/range1797086/1
type: Range
persistent identity: range1797086
display id: range1797086
version: 1
top level: TSS
direction: direction/forward
start: 10
end: 12
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0043
display id: BBa_B0043
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (forward)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Nt.BstNB I recognition site
sequence: https://synbiohub.org/public/igem/BBa_B0043_sequence/1

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797101
display id: annotation1797101
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797101/range1797101/1
type: Range
persistent identity: range1797101
display id: range1797101
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797104
display id: annotation1797104
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797104/range1797104/1
type: Range
persistent identity: range1797104
display id: range1797104
version: 1
top level: TOPO
direction: direction/forward
start: 5
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797103
display id: annotation1797103
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797103/range1797103/1
type: Range
persistent identity: range1797103
display id: range1797103
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797102
display id: annotation1797102
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0043/annotation1797102/range1797102/1
type: Range
persistent identity: range1797102
display id: range1797102
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

NheI
type: ComponentDefinition
persistent identity: BBa B0045
display id: BBa_B0045
version: 1
was generated by: iGEM to SBOL conversion
description: NheI restriction enzyme site (XbaI, SpeI compatible overhang)
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
discontinued: false
dominant: true
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: NheI site
sequence: https://synbiohub.org/public/igem/BBa_B0045_sequence/1

NheI site
type: SequenceAnnotation
persistent identity: annotation1822724
display id: annotation1822724
version: 1
top level: NheI
location: https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0045/annotation1822724/range1822724/1
type: Range
persistent identity: range1822724
display id: range1822724
version: 1
top level: NheI
direction: direction/forward
start: 1
end: 6
orientation: inline

BBa_B0053
type: ComponentDefinition
persistent identity: BBa B0053
display id: BBa_B0053
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (His)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator from the E.coli His operon
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0053_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318602
display id: annotation318602
version: 1
top level: BBa_B0053
location: https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0053/annotation318602/range318602/1
type: Range
persistent identity: range318602
display id: range318602
version: 1
top level: BBa_B0053
direction: direction/forward
start: 9
end: 43
orientation: inline

BBa_B0055
type: ComponentDefinition
persistent identity: BBa B0055
display id: BBa_B0055
version: 1
was generated by: iGEM to SBOL conversion
description: -- No description --
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to bacteriophage T3
sequence annotation: stem loop from bacteriophage T3
sequence: https://synbiohub.org/public/igem/BBa_B0055_sequence/1

identical to bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783311
display id: annotation1783311
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0055/annotation1783311/range1783311/1
type: Range
persistent identity: range1783311
display id: range1783311
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 28
end: 66
orientation: inline

stem loop from bacteriophage T3
type: SequenceAnnotation
persistent identity: annotation1783301
display id: annotation1783301
version: 1
top level: BBa_B0055
location: https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0055/annotation1783301/range1783301/1
type: Range
persistent identity: range1783301
display id: range1783301
version: 1
top level: BBa_B0055
direction: direction/reverse
start: 36
end: 57
orientation: inline

Prefix
type: ComponentDefinition
persistent identity: BBa G00000
display id: BBa_G00000
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site prefix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks prefix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Prefix
sequence annotation: EcoRI site
sequence annotation: NotI site
sequence annotation: XbaI site
sequence: https://synbiohub.org/public/igem/BBa_G00000_sequence/1

Prefix
type: SequenceAnnotation
persistent identity: annotation1934225
display id: annotation1934225
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_G00000/annotation1934225/range1934225/1
type: Range
persistent identity: range1934225
display id: range1934225
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 22
orientation: inline

EcoRI site
type: SequenceAnnotation
persistent identity: annotation1934223
display id: annotation1934223
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934223/range1934223/1
type: Range
persistent identity: range1934223
display id: range1934223
version: 1
top level: Prefix
direction: direction/forward
start: 1
end: 6
orientation: inline

NotI site
type: SequenceAnnotation
persistent identity: annotation1961228
display id: annotation1961228
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1961228/range1961228/1
type: Range
persistent identity: range1961228
display id: range1961228
version: 1
top level: Prefix
direction: direction/forward
start: 7
end: 14
orientation: inline

XbaI site
type: SequenceAnnotation
persistent identity: annotation1934224
display id: annotation1934224
version: 1
top level: Prefix
location: https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00000/annotation1934224/range1934224/1
type: Range
persistent identity: range1934224
display id: range1934224
version: 1
top level: Prefix
direction: direction/forward
start: 16
end: 21
orientation: inline

VF2
type: ComponentDefinition
persistent identity: BBa G00100
display id: BBa_G00100
version: 1
was generated by: iGEM to SBOL conversion
description: Forward primer for sequencing/amplifying BioBrick parts (VF2)
created: 2004-05-24T11:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 60
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Tom Knight
type: DnaRegion
role: SO:0000112
role: partType/Primer
sequence annotation: VF2
sequence: https://synbiohub.org/public/igem/BBa_G00100_sequence/1

VF2
type: SequenceAnnotation
persistent identity: annotation1934505
display id: annotation1934505
version: 1
top level: VF2
location: https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
role: SO:0005850
role: feature/primer binding

https://synbiohub.org/public/igem/BBa_G00100/annotation1934505/range1934505/1
type: Range
persistent identity: range1934505
display id: range1934505
version: 1
top level: VF2
direction: direction/forward
start: 1
end: 20
orientation: inline

ampR
type: ComponentDefinition
persistent identity: BBa P1006
display id: BBa_P1006
version: 1
was generated by: iGEM to SBOL conversion
description: ampicillin resistance cassette, reverse orientation
created: 2006-02-03T12:00:00Z
modified: 2015-05-08T01:14:11Z
owned by: james
owned by: myers
mutable description: This contains TetR, a terminator and then AmpR and a terminator
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: ampicillin resistance
sequence annotation: ampR RBS
sequence annotation: ampR -10
sequence annotation: ampR -35
sequence: https://synbiohub.org/public/igem/BBa_P1006_sequence/1

ampicillin resistance
type: SequenceAnnotation
persistent identity: annotation1897231
display id: annotation1897231
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897231/range1897231/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1006/annotation1897231/range1897231/1
type: Range
persistent identity: range1897231
display id: range1897231
version: 1
top level: ampR
direction: direction/reverse
start: 1
end: 864
orientation: inline

ampR RBS
type: SequenceAnnotation
persistent identity: annotation1897232
display id: annotation1897232
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897232/range1897232/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_P1006/annotation1897232/range1897232/1
type: Range
persistent identity: range1897232
display id: range1897232
version: 1
top level: ampR
direction: direction/reverse
start: 869
end: 873
orientation: inline

ampR -10
type: SequenceAnnotation
persistent identity: annotation1897233
display id: annotation1897233
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897233/range1897233/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1006/annotation1897233/range1897233/1
type: Range
persistent identity: range1897233
display id: range1897233
version: 1
top level: ampR
direction: direction/reverse
start: 882
end: 887
orientation: inline

ampR -35
type: SequenceAnnotation
persistent identity: annotation1897234
display id: annotation1897234
version: 1
top level: ampR
location: https://synbiohub.org/public/igem/BBa_P1006/annotation1897234/range1897234/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1006/annotation1897234/range1897234/1
type: Range
persistent identity: range1897234
display id: range1897234
version: 1
top level: ampR
direction: direction/reverse
start: 908
end: 913
orientation: inline

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50022
display id: BBa_I50022
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal pUC19-derived high copy replication origin
created: 2008-01-05T12:00:00Z
modified: 2015-08-31T04:07:42Z
mutable provenance: pSB1A3
owned by: james
owned by: myers
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: rep (pMB1)
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: origin of replication
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: RNAI transcript
sequence annotation: RNAI transcript promoter -10
sequence annotation: RNAI transcript promoter -35
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_I50022_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1959117
display id: annotation1959117
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50022/annotation1959117/range1959117/1
type: Range
persistent identity: range1959117
display id: range1959117
version: 1
top level: pUC19 ori
direction: direction/forward
start: 16
end: 630
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1959118
display id: annotation1959118
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50022/annotation1959118/range1959118/1
type: Range
persistent identity: range1959118
display id: range1959118
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 32
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1959119
display id: annotation1959119
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50022/annotation1959119/range1959119/1
type: Range
persistent identity: range1959119
display id: range1959119
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 615
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1959120
display id: annotation1959120
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50022/annotation1959120/range1959120/1
type: Range
persistent identity: range1959120
display id: range1959120
version: 1
top level: pUC19 ori
direction: direction/forward
start: 48
end: 53
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1959121
display id: annotation1959121
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50022/annotation1959121/range1959121/1
type: Range
persistent identity: range1959121
display id: range1959121
version: 1
top level: pUC19 ori
direction: direction/forward
start: 63
end: 615
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1959122
display id: annotation1959122
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50022/annotation1959122/range1959122/1
type: Range
persistent identity: range1959122
display id: range1959122
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 66
end: 173
orientation: inline

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1959123
display id: annotation1959123
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50022/annotation1959123/range1959123/1
type: Range
persistent identity: range1959123
display id: range1959123
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 182
end: 187
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1959124
display id: annotation1959124
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50022/annotation1959124/range1959124/1
type: Range
persistent identity: range1959124
display id: range1959124
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 204
end: 209
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1959125
display id: annotation1959125
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50022/annotation1959125/range1959125/1
type: Range
persistent identity: range1959125
display id: range1959125
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 615
end: 615
orientation: inline

ccdB
type: ComponentDefinition
persistent identity: BBa P1016
display id: BBa_P1016
version: 1
was generated by: iGEM to SBOL conversion
description: ccdB cassette without ccdA-
created: 2007-01-29T12:00:00Z
modified: 2015-06-15T01:13:47Z
mutable provenance: This part is derived from BBa_P1010 but it lacks ccdA-.
owned by: james
owned by: myers
mutable description: This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
discontinued: true
dominant: false
experience: experience/Works
group u list: _41_
m user id: 4206
owner id: 126
owning group id: 70
sample status: Not in stock
status: status/Deleted
bookmark: false
mutable notes: ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Generator
role: SO:0000804
sequence annotation: -35
sequence annotation: -10
sequence annotation: ccdB
sequence: https://synbiohub.org/public/igem/BBa_P1016_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation1910684
display id: annotation1910684
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1016/annotation1910684/range1910684/1
type: Range
persistent identity: range1910684
display id: range1910684
version: 1
top level: ccdB
direction: direction/forward
start: 41
end: 46
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1910685
display id: annotation1910685
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_P1016/annotation1910685/range1910685/1
type: Range
persistent identity: range1910685
display id: range1910685
version: 1
top level: ccdB
direction: direction/forward
start: 64
end: 69
orientation: inline

ccdB
type: SequenceAnnotation
persistent identity: annotation1910686
display id: annotation1910686
version: 1
top level: ccdB
location: https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_P1016/annotation1910686/range1910686/1
type: Range
persistent identity: range1910686
display id: range1910686
version: 1
top level: ccdB
direction: direction/forward
start: 108
end: 416
orientation: inline

TOPO
type: ComponentDefinition
persistent identity: BBa B0044
display id: BBa_B0044
version: 1
was generated by: iGEM to SBOL conversion
description: Topoisomerase I mediated cloning site (reverse)
created: 2006-02-04T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: Nt.BstNB I recognition site
sequence annotation: Arbitrary sequence (could be any bases)
sequence annotation: Topoisomerase I recognition site
sequence annotation: Generated T overhang
sequence: https://synbiohub.org/public/igem/BBa_B0044_sequence/1

Nt.BstNB I recognition site
type: SequenceAnnotation
persistent identity: annotation1797126
display id: annotation1797126
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797126/range1797126/1
type: Range
persistent identity: range1797126
display id: range1797126
version: 1
top level: TOPO
direction: direction/forward
start: 1
end: 5
orientation: inline

Arbitrary sequence (could be any bases)
type: SequenceAnnotation
persistent identity: annotation1797127
display id: annotation1797127
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0044/annotation1797127/range1797127/1
type: Range
persistent identity: range1797127
display id: range1797127
version: 1
top level: TOPO
direction: direction/forward
start: 6
end: 8
orientation: inline

Topoisomerase I recognition site
type: SequenceAnnotation
persistent identity: annotation1797128
display id: annotation1797128
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797128/range1797128/1
type: Range
persistent identity: range1797128
display id: range1797128
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 13
orientation: inline

Generated T overhang
type: SequenceAnnotation
persistent identity: annotation1797129
display id: annotation1797129
version: 1
top level: TOPO
location: https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_B0044/annotation1797129/range1797129/1
type: Range
persistent identity: range1797129
display id: range1797129
version: 1
top level: TOPO
direction: direction/reverse
start: 9
end: 9
orientation: inline

BBa_B0054
type: ComponentDefinition
persistent identity: BBa B0054
display id: BBa_B0054
version: 1
was generated by: iGEM to SBOL conversion
description: Transcriptional terminator
created: 2005-11-16T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
discontinued: false
dominant: false
experience: experience/None
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: identical to E. coli MG1655 between tonB and yciA
sequence annotation: stem loop
sequence: https://synbiohub.org/public/igem/BBa_B0054_sequence/1

identical to E. coli MG1655 between tonB and yciA
type: SequenceAnnotation
persistent identity: annotation1783342
display id: annotation1783342
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_B0054/annotation1783342/range1783342/1
type: Range
persistent identity: range1783342
display id: range1783342
version: 1
top level: BBa_B0054
direction: direction/forward
start: 4
end: 43
orientation: inline

stem loop
type: SequenceAnnotation
persistent identity: annotation1747359
display id: annotation1747359
version: 1
top level: BBa_B0054
location: https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0054/annotation1747359/range1747359/1
type: Range
persistent identity: range1747359
display id: range1747359
version: 1
top level: BBa_B0054
direction: direction/forward
start: 11
end: 42
orientation: inline

BBa_B0062
type: ComponentDefinition
persistent identity: BBa B0062
display id: BBa_B0062
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (Reverse B0052)
created: 2004-01-29T12:00:00Z
modified: 2015-08-31T04:07:21Z
owned by: james
owned by: myers
mutable description: Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Caitlin Conboy
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0062_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation318637
display id: annotation318637
version: 1
top level: BBa_B0062
location: https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0062/annotation318637/range318637/1
type: Range
persistent identity: range318637
display id: range318637
version: 1
top level: BBa_B0062
direction: direction/forward
start: 14
end: 33
orientation: inline

Suffix
type: ComponentDefinition
persistent identity: BBa G00001
display id: BBa_G00001
version: 1
was generated by: iGEM to SBOL conversion
description: BioBrick cloning site suffix
created: 2006-03-13T12:00:00Z
modified: 2015-08-31T04:07:27Z
owned by: james
owned by: myers
mutable description: This part is the standard BioBricks suffix.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: Suffix
sequence annotation: SpeI
sequence annotation: NotI
sequence annotation: PstI
sequence: https://synbiohub.org/public/igem/BBa_G00001_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation1821544
display id: annotation1821544
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_G00001/annotation1821544/range1821544/1
type: Range
persistent identity: range1821544
display id: range1821544
version: 1
top level: Suffix
direction: direction/forward
start: 1
end: 21
orientation: inline

SpeI
type: SequenceAnnotation
persistent identity: annotation1934226
display id: annotation1934226
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_G00001/annotation1934226/range1934226/1
type: Range
persistent identity: range1934226
display id: range1934226
version: 1
top level: Suffix
direction: direction/forward
start: 2
end: 7
orientation: inline

NotI
type: SequenceAnnotation
persistent identity: annotation1934228
display id: annotation1934228
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934228/range1934228/1
type: Range
persistent identity: range1934228
display id: range1934228
version: 1
top level: Suffix
direction: direction/forward
start: 9
end: 16
orientation: inline

PstI
type: SequenceAnnotation
persistent identity: annotation1934227
display id: annotation1934227
version: 1
top level: Suffix
location: https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_G00001/annotation1934227/range1934227/1
type: Range
persistent identity: range1934227
display id: range1934227
version: 1
top level: Suffix
direction: direction/forward
start: 16
end: 21
orientation: inline

VR
type: ComponentDefinition
persistent identity: BBa G00102
display id: BBa_G00102
version: 1
was generated by: iGEM to SBOL conversion
description: Reverse BioBrick primer annealing site (VR binding site)
created: 2006-02-03T12:00:00Z
modified: 2015-08-31T04:07:28Z
owned by: james
owned by: myers
mutable description: This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 45
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: VR
sequence: https://synbiohub.org/public/igem/BBa_G00102_sequence/1

VR
type: SequenceAnnotation
persistent identity: annotation1961229
display id: annotation1961229
version: 1
top level: VR
location: https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_G00102/annotation1961229/range1961229/1
type: Range
persistent identity: range1961229
display id: range1961229
version: 1
top level: VR
direction: direction/reverse
start: 1
end: 20
orientation: inline

pUC19 ori
type: ComponentDefinition
persistent identity: BBa I50020
display id: BBa_I50020
version: 1
was generated by: iGEM to SBOL conversion
description: Minimal pUC19-derived high copy replication origin
created: 2006-01-09T12:00:00Z
modified: 2015-08-31T04:07:41Z
owned by: james
owned by: myers
mutable description: High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _41_6_
m user id: 0
owner id: 126
owning group id: 44
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: rep (pMB1)
sequence annotation: RNAII transcript promoter -35 sequence
sequence annotation: origin of replication
sequence annotation: RNAII transcript promoter -10 sequence
sequence annotation: RNAII transcript
sequence annotation: RNAI transcript
sequence annotation: RNAI transcript promoter -10
sequence annotation: RNAI transcript promoter -35
sequence annotation: G to A, nonfunctional mutation
sequence annotation: C to T, nonfunctional mutation
sequence annotation: ORI
sequence: https://synbiohub.org/public/igem/BBa_I50020_sequence/1

rep (pMB1)
type: SequenceAnnotation
persistent identity: annotation1782180
display id: annotation1782180
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1782180/range1782180/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50020/annotation1782180/range1782180/1
type: Range
persistent identity: range1782180
display id: range1782180
version: 1
top level: pUC19 ori
direction: direction/forward
start: 16
end: 630
orientation: inline

RNAII transcript promoter -35 sequence
type: SequenceAnnotation
persistent identity: annotation1793686
display id: annotation1793686
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793686/range1793686/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793686/range1793686/1
type: Range
persistent identity: range1793686
display id: range1793686
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 32
orientation: inline

origin of replication
type: SequenceAnnotation
persistent identity: annotation1793680
display id: annotation1793680
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793680/range1793680/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1793680/range1793680/1
type: Range
persistent identity: range1793680
display id: range1793680
version: 1
top level: pUC19 ori
direction: direction/forward
start: 27
end: 615
orientation: inline

RNAII transcript promoter -10 sequence
type: SequenceAnnotation
persistent identity: annotation1793685
display id: annotation1793685
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793685/range1793685/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793685/range1793685/1
type: Range
persistent identity: range1793685
display id: range1793685
version: 1
top level: pUC19 ori
direction: direction/forward
start: 48
end: 53
orientation: inline

RNAII transcript
type: SequenceAnnotation
persistent identity: annotation1793681
display id: annotation1793681
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793681/range1793681/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_I50020/annotation1793681/range1793681/1
type: Range
persistent identity: range1793681
display id: range1793681
version: 1
top level: pUC19 ori
direction: direction/forward
start: 63
end: 615
orientation: inline

RNAI transcript
type: SequenceAnnotation
persistent identity: annotation1793684
display id: annotation1793684
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793684/range1793684/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1793684/range1793684/1
type: Range
persistent identity: range1793684
display id: range1793684
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 66
end: 173
orientation: inline

RNAI transcript promoter -10
type: SequenceAnnotation
persistent identity: annotation1793683
display id: annotation1793683
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793683/range1793683/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793683/range1793683/1
type: Range
persistent identity: range1793683
display id: range1793683
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 182
end: 187
orientation: inline

RNAI transcript promoter -35
type: SequenceAnnotation
persistent identity: annotation1793682
display id: annotation1793682
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1793682/range1793682/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I50020/annotation1793682/range1793682/1
type: Range
persistent identity: range1793682
display id: range1793682
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 204
end: 209
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910687
display id: annotation1910687
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910687/range1910687/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910687/range1910687/1
type: Range
persistent identity: range1910687
display id: range1910687
version: 1
top level: pUC19 ori
direction: direction/forward
start: 261
end: 261
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910688
display id: annotation1910688
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910688/range1910688/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910688/range1910688/1
type: Range
persistent identity: range1910688
display id: range1910688
version: 1
top level: pUC19 ori
direction: direction/forward
start: 265
end: 265
orientation: inline

G to A, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910689
display id: annotation1910689
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910689/range1910689/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910689/range1910689/1
type: Range
persistent identity: range1910689
display id: range1910689
version: 1
top level: pUC19 ori
direction: direction/forward
start: 274
end: 274
orientation: inline

C to T, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910690
display id: annotation1910690
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910690/range1910690/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910690/range1910690/1
type: Range
persistent identity: range1910690
display id: range1910690
version: 1
top level: pUC19 ori
direction: direction/forward
start: 303
end: 303
orientation: inline

C to T, nonfunctional mutation
type: SequenceAnnotation
persistent identity: annotation1910691
display id: annotation1910691
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1910691/range1910691/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I50020/annotation1910691/range1910691/1
type: Range
persistent identity: range1910691
display id: range1910691
version: 1
top level: pUC19 ori
direction: direction/forward
start: 467
end: 467
orientation: inline

ORI
type: SequenceAnnotation
persistent identity: annotation1782181
display id: annotation1782181
version: 1
top level: pUC19 ori
location: https://synbiohub.org/public/igem/BBa_I50020/annotation1782181/range1782181/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I50020/annotation1782181/range1782181/1
type: Range
persistent identity: range1782181
display id: range1782181
version: 1
top level: pUC19 ori
direction: direction/reverse
start: 615
end: 615
orientation: inline

https://synbiohub.org/public/igem/BBa_I51019_sequence/1
type: Sequence
persistent identity: BBa I51019 sequence
display id: BBa_I51019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctttcgctaaggatgatttctgctagcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttaggctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttctagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0042_sequence/1
type: Sequence
persistent identity: BBa B0042 sequence
display id: BBa_B0042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttagttagttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0043_sequence/1
type: Sequence
persistent identity: BBa B0043 sequence
display id: BBa_B0043_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccttagtgactc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0045_sequence/1
type: Sequence
persistent identity: BBa B0045 sequence
display id: BBa_B0045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0053_sequence/1
type: Sequence
persistent identity: BBa B0053 sequence
display id: BBa_B0053_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgtt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0055_sequence/1
type: Sequence
persistent identity: BBa B0055 sequence
display id: BBa_B0055_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaggaatattcagcaatttgcccgtgccgaagaaaggcccacccgtgaaggtgagccagtgagttgattgctacgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00000_sequence/1
type: Sequence
persistent identity: BBa G00000 sequence
display id: BBa_G00000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaattcgcggccgcttctagag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00100_sequence/1
type: Sequence
persistent identity: BBa G00100 sequence
display id: BBa_G00100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tgccacctgacgtctaagaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1006_sequence/1
type: Sequence
persistent identity: BBa P1006 sequence
display id: BBa_P1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgttcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaataataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaagagtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgttgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggggcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatagatatttgaatgtatttag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50022_sequence/1
type: Sequence
persistent identity: BBa I50022 sequence
display id: BBa_I50022_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P1016_sequence/1
type: Sequence
persistent identity: BBa P1016 sequence
display id: BBa_P1016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctgatgttctggggaatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0044_sequence/1
type: Sequence
persistent identity: BBa B0044 sequence
display id: BBa_B0044_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagtcactaaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0054_sequence/1
type: Sequence
persistent identity: BBa B0054 sequence
display id: BBa_B0054_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attagcagaaagtcaaaagcctccgaccggaggcttttgactaaaacttcccttggggttatcattggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0062_sequence/1
type: Sequence
persistent identity: BBa B0062 sequence
display id: BBa_B0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cagataaaaaaaatccttagctttcgctaaggatgatttct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00001_sequence/1
type: Sequence
persistent identity: BBa G00001 sequence
display id: BBa_G00001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_G00102_sequence/1
type: Sequence
persistent identity: BBa G00102 sequence
display id: BBa_G00102_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctcactcaaaggcggtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I50020_sequence/1
type: Sequence
persistent identity: BBa I50020 sequence
display id: BBa_I50020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccgggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z