LOCUS BBa_I51020 2438 bp DNA linear 04-May-2024 DEFINITION BioBrick base vector (for constructing pSB**5 vectors) ACCESSION BBa_I51020 VERSION BBa_I51020.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=Suffix misc_feature 2..7 /label=SpeI misc_feature 16..21 /label=PstI misc_feature 9..16 /label=NotI misc_feature 1..21 /label=BBa_G00001 misc_feature 22..26 /label=Nt.BstNB I recognition site misc_feature 27..29 /label=Arbitrary sequence (could be any bases) misc_feature 30..34 /label=Topoisomerase I recognition site misc_feature 30..30 /label=Generated T overhang misc_feature 22..34 /label=BBa_B0044 misc_feature 36..38 /label=stop codon (+2 frame) misc_feature 35..37 /label=stop codon (-1 frame) misc_feature 40..42 /label=stop codon (+3 frame) misc_feature 44..46 /label=stop codon (+1 frame) misc_feature 43..45 /label=stop codon (-2 frame) misc_feature 39..41 /label=stop codon (-3 frame) misc_feature 35..46 /label=BBa_B0042 stem_loop 57..88 /label=stem loop misc_feature 50..89 /label=identical to E. coli MG1655 between tonB and yciA terminator 47..115 /label=BBa_B0054 primer_bind 116..135 /label=VR misc_feature 116..135 /label=BBa_G00102 stem_loop 149..168 /label=stem_loop terminator 136..176 /label=BBa_B0062 misc_feature 177..182 /label=NheI site misc_feature 177..182 /label=BBa_B0045 CDS 183..1046 /label=ampicillin resistance RBS 1051..1055 /label=ampR RBS promoter 1064..1069 /label=ampR -10 promoter 1090..1095 /label=ampR -35 misc_feature 183..1125 /label=BBa_P1006 misc_feature 1126..1131 /label=NheI site misc_feature 1126..1131 /label=BBa_B0045 stem_loop 1140..1174 /label=stem_loop terminator 1132..1203 /label=BBa_B0053 primer_bind 1204..1223 /label=VF2 primer 1204..1223 /label=BBa_G00100 stem_loop 1259..1280 /label=stem loop from bacteriophage T3 misc_feature 1251..1289 /label=identical to bacteriophage T3 terminator 1224..1301 /label=BBa_B0055 misc_feature 1303..1305 /label=stop codon (+2 frame) misc_feature 1302..1304 /label=stop codon (-1 frame) misc_feature 1307..1309 /label=stop codon (+3 frame) misc_feature 1311..1313 /label=stop codon (+1 frame) misc_feature 1310..1312 /label=stop codon (-2 frame) misc_feature 1306..1308 /label=stop codon (-3 frame) misc_feature 1302..1313 /label=BBa_B0042 misc_feature 1314..1318 /label=Topoisomerase I recognition site misc_feature 1322..1326 /label=Nt.BstNB I recognition site misc_feature 1319..1321 /label=Arbitrary sequence (could be any bases) misc_feature 1318..1318 /label=Generated T overhang misc_feature 1314..1326 /label=BBa_B0043 misc_feature 1327..1332 /label=EcoRI site misc_feature 1342..1347 /label=XbaI site misc_feature 1327..1348 /label=Prefix misc_feature 1333..1340 /label=NotI site misc_feature 1327..1348 /label=BBa_G00000 promoter 1389..1394 /label=-35 promoter 1412..1417 /label=-10 CDS 1456..1764 /label=ccdB misc_feature 1349..1764 /label=BBa_P1016 misc_feature 1780..2394 /label=rep (pMB1) promoter 1791..1796 /label=RNAII transcript promoter -35 sequence misc_feature 1791..2379 /label=origin of replication promoter 1812..1817 /label=RNAII transcript promoter -10 sequence misc_feature 1827..2379 /label=RNAII transcript misc_feature 1830..1937 /label=RNAI transcript promoter 1946..1951 /label=RNAI transcript promoter -10 promoter 1968..1973 /label=RNAI transcript promoter -35 misc_feature 2379..2379 /label=ORI misc_feature 1765..2438 /label=BBa_I50022 ORIGIN 1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc 61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca 121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg atttctgcta 181 gcttattacc aatgcttaat cagggaggca cctatctcag cgatctggcg attacgttcg 241 tccatagttg cctggctccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 301 ggccccaggg ctgcaataat accgcgggac ccacgctcac cggctccaga tttatcagca 361 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 421 atccagtcta ttaattgttg ccgggaagcg agagtaagaa gttcgccagt taagagtttg 481 cgcaacgttg ttgccatagc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 541 tcattcagct ccggttccca acgatcaagg cgagttacat gatcacccat gttgtgcaaa 601 aaagcggtaa gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgccgtgtta 661 tcactcatgg ttatggcagc gctgcataat tcgcgtactg tcatgccgtc cgtaagatgc 721 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 781 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacagagcag aactttaaaa 841 gtgctcatca ttgggaaacg ttcttcgggg cgaaaactct caagaatctt accgctgttg 901 aggtccagtt cgatgtaacc cacacgtgca cccaactgat cttcggcatc ttttactttc 961 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 1021 gcgacacgga aatgttgaat actcatattc ttcctttttc aatattattg aagcatttat 1081 cagggttatt gtctcatgag cggatagata tttgaatgta tttaggctag ctccggcaaa 1141 aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag attacttcgc 1201 gtttgccacc tgacgtctaa gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg 1261 cccacccgtg aaggtgagcc agtgagttga ttgctacgta attagttagt tagcccttag 1321 tgactcgaat tcgcggccgc ttctagaggg cttactaaaa gccagataac agtatgcgta 1381 tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc gaagtatgtc 1441 aaaaagaggt atgctatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 1501 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc 1561 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 1621 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 1681 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 1741 ctgatgttct ggggaatata ataacccgta gaaaagatca aaagatcttc ttgagatcct 1801 ttttttctgc gcgtaatctg ctacttgcaa acaaaaaaac caccgctacc agcggtggtt 1861 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt tagcagagcg 1921 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 1981 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 2041 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 2101 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 2161 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 2221 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 2281 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 2341 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 2401 ttacggttcc tggccttttg ctggcctttt gctcacat //