##gff-version 3
##sequence-region BBa_I51020 1 2438
BBa_I51020	.	sequence_feature	1765	2438	.	+	0	ID=BBa_I50022;Name=BBa_I50022
BBa_I51020	.	sequence_feature	2379	2379	.	+	0	ID=annotation1959125;Name=ORI;Parent=BBa_I50022
BBa_I51020	.	promoter	1968	1973	.	+	0	ID=annotation1959124;Name=RNAI transcript promoter -35;Parent=BBa_I50022
BBa_I51020	.	promoter	1812	1817	.	+	0	ID=annotation1959120;Name=RNAII transcript promoter -10 sequence;Parent=BBa_I50022
BBa_I51020	.	promoter	1791	1796	.	+	0	ID=annotation1959118;Name=RNAII transcript promoter -35 sequence;Parent=BBa_I50022
BBa_I51020	.	sequence_feature	1827	2379	.	+	0	ID=annotation1959121;Name=RNAII transcript;Parent=BBa_I50022
BBa_I51020	.	sequence_feature	1780	2394	.	+	0	ID=annotation1959117;Name=rep (pMB1);Parent=BBa_I50022
BBa_I51020	.	sequence_feature	1791	2379	.	+	0	ID=annotation1959119;Name=origin of replication;Parent=BBa_I50022
BBa_I51020	.	promoter	1946	1951	.	+	0	ID=annotation1959123;Name=RNAI transcript promoter -10;Parent=BBa_I50022
BBa_I51020	.	sequence_feature	1830	1937	.	+	0	ID=annotation1959122;Name=RNAI transcript;Parent=BBa_I50022
BBa_I51020	.	terminator	136	176	.	+	0	ID=BBa_B0062;Name=BBa_B0062
BBa_I51020	.	stem_loop	149	168	.	+	0	ID=annotation318637;Name=stem_loop;Parent=BBa_B0062
BBa_I51020	.	sequence_feature	35	46	.	+	0	ID=BBa_B0042;Name=BBa_B0042
BBa_I51020	.	stop_codon	40	42	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	39	41	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	35	37	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	44	46	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	43	45	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	36	38	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
BBa_I51020	.	primer	1204	1223	.	+	0	ID=BBa_G00100;Name=BBa_G00100
BBa_I51020	.	primer_binding_site	1204	1223	.	+	0	ID=annotation1934505;Name=VF2;Parent=BBa_G00100
BBa_I51020	.	engineered_region	1349	1764	.	+	0	ID=BBa_P1016;Name=BBa_P1016
BBa_I51020	.	promoter	1389	1394	.	+	0	ID=annotation1910684;Name=-35;Parent=BBa_P1016
BBa_I51020	.	promoter	1412	1417	.	+	0	ID=annotation1910685;Name=-10;Parent=BBa_P1016
BBa_I51020	.	CDS	1456	1764	.	+	0	ID=annotation1910686;Name=ccdB;Parent=BBa_P1016
BBa_I51020	.	sequence_feature	1302	1313	.	+	0	ID=BBa_B0042;Name=BBa_B0042
BBa_I51020	.	stop_codon	1307	1309	.	+	0	ID=annotation1797085;Name=stop codon (+3 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	1306	1308	.	+	0	ID=annotation1797088;Name=stop codon (-3 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	1302	1304	.	+	0	ID=annotation1797084;Name=stop codon (-1 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	1311	1313	.	+	0	ID=annotation1797086;Name=stop codon (+1 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	1310	1312	.	+	0	ID=annotation1797087;Name=stop codon (-2 frame);Parent=BBa_B0042
BBa_I51020	.	stop_codon	1303	1305	.	+	0	ID=annotation1797083;Name=stop codon (+2 frame);Parent=BBa_B0042
BBa_I51020	.	sequence_feature	22	34	.	+	0	ID=BBa_B0044;Name=BBa_B0044
BBa_I51020	.	sequence_feature	30	30	.	+	0	ID=annotation1797129;Name=Generated T overhang;Parent=BBa_B0044
BBa_I51020	.	sequence_feature	22	26	.	+	0	ID=annotation1797126;Name=Nt.BstNB I recognition site;Parent=BBa_B0044
BBa_I51020	.	sequence_feature	30	34	.	+	0	ID=annotation1797128;Name=Topoisomerase I recognition site;Parent=BBa_B0044
BBa_I51020	.	sequence_feature	27	29	.	+	0	ID=annotation1797127;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0044
BBa_I51020	.	sequence_feature	1314	1326	.	+	0	ID=BBa_B0043;Name=BBa_B0043
BBa_I51020	.	sequence_feature	1319	1321	.	+	0	ID=annotation1797103;Name=Arbitrary sequence (could be any bases);Parent=BBa_B0043
BBa_I51020	.	sequence_feature	1314	1318	.	+	0	ID=annotation1797101;Name=Topoisomerase I recognition site;Parent=BBa_B0043
BBa_I51020	.	sequence_feature	1322	1326	.	+	0	ID=annotation1797102;Name=Nt.BstNB I recognition site;Parent=BBa_B0043
BBa_I51020	.	sequence_feature	1318	1318	.	+	0	ID=annotation1797104;Name=Generated T overhang;Parent=BBa_B0043
BBa_I51020	.	sequence_feature	1	21	.	+	0	ID=BBa_G00001;Name=BBa_G00001
BBa_I51020	.	sequence_feature	9	16	.	+	0	ID=annotation1934228;Name=NotI;Parent=BBa_G00001
BBa_I51020	.	engineered_region	1	21	.	+	0	ID=annotation1821544;Name=Suffix;Parent=BBa_G00001
BBa_I51020	.	sequence_feature	2	7	.	+	0	ID=annotation1934226;Name=SpeI;Parent=BBa_G00001
BBa_I51020	.	sequence_feature	16	21	.	+	0	ID=annotation1934227;Name=PstI;Parent=BBa_G00001
BBa_I51020	.	sequence_feature	116	135	.	+	0	ID=BBa_G00102;Name=BBa_G00102
BBa_I51020	.	primer_binding_site	116	135	.	+	0	ID=annotation1961229;Name=VR;Parent=BBa_G00102
BBa_I51020	.	sequence_feature	1327	1348	.	+	0	ID=BBa_G00000;Name=BBa_G00000
BBa_I51020	.	sequence_feature	1342	1347	.	+	0	ID=annotation1934224;Name=XbaI site;Parent=BBa_G00000
BBa_I51020	.	sequence_feature	1333	1340	.	+	0	ID=annotation1961228;Name=NotI site;Parent=BBa_G00000
BBa_I51020	.	engineered_region	1327	1348	.	+	0	ID=annotation1934225;Name=Prefix;Parent=BBa_G00000
BBa_I51020	.	sequence_feature	1327	1332	.	+	0	ID=annotation1934223;Name=EcoRI site;Parent=BBa_G00000
BBa_I51020	.	engineered_region	183	1125	.	+	0	ID=BBa_P1006;Name=BBa_P1006
BBa_I51020	.	CDS	183	1046	.	+	0	ID=annotation1897231;Name=ampicillin resistance;Parent=BBa_P1006
BBa_I51020	.	ribosome_entry_site	1051	1055	.	+	0	ID=annotation1897232;Name=ampR RBS;Parent=BBa_P1006
BBa_I51020	.	promoter	1064	1069	.	+	0	ID=annotation1897233;Name=ampR -10;Parent=BBa_P1006
BBa_I51020	.	promoter	1090	1095	.	+	0	ID=annotation1897234;Name=ampR -35;Parent=BBa_P1006
BBa_I51020	.	terminator	1132	1203	.	+	0	ID=BBa_B0053;Name=BBa_B0053
BBa_I51020	.	stem_loop	1140	1174	.	+	0	ID=annotation318602;Name=stem_loop;Parent=BBa_B0053
BBa_I51020	.	sequence_feature	1126	1131	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_I51020	.	sequence_feature	1126	1131	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
BBa_I51020	.	terminator	47	115	.	+	0	ID=BBa_B0054;Name=BBa_B0054
BBa_I51020	.	sequence_feature	50	89	.	+	0	ID=annotation1783342;Name=identical to E. coli MG1655 between tonB and yciA;Parent=BBa_B0054
BBa_I51020	.	stem_loop	57	88	.	+	0	ID=annotation1747359;Name=stem loop;Parent=BBa_B0054
BBa_I51020	.	terminator	1224	1301	.	+	0	ID=BBa_B0055;Name=BBa_B0055
BBa_I51020	.	stem_loop	1259	1280	.	+	0	ID=annotation1783301;Name=stem loop from bacteriophage T3;Parent=BBa_B0055
BBa_I51020	.	sequence_feature	1251	1289	.	+	0	ID=annotation1783311;Name=identical to bacteriophage T3;Parent=BBa_B0055
BBa_I51020	.	sequence_feature	177	182	.	+	0	ID=BBa_B0045;Name=BBa_B0045
BBa_I51020	.	sequence_feature	177	182	.	+	0	ID=annotation1822724;Name=NheI site;Parent=BBa_B0045
>BBa_I51020
tactagtagcggccgctgcaggagtcactaagggttagttagttagattagcagaaagtcaaaagcctccgaccggagg
cttttgactaaaacttcccttggggttatcattggggctcactcaaaggcggtaatcagataaaaaaaatccttagctt
tcgctaaggatgatttctgctagcttattaccaatgcttaatcagggaggcacctatctcagcgatctggcgattacgt
tcgtccatagttgcctggctccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagggctgcaa
taataccgcgggacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaag
tggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagcgagagtaagaagttcgccagttaag
agtttgcgcaacgttgttgccatagctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg
gttcccaacgatcaaggcgagttacatgatcacccatgttgtgcaaaaaagcggtaagctccttcggtcctccgatcgt
tgtcagaagtaagttggccgccgtgttatcactcatggttatggcagcgctgcataattcgcgtactgtcatgccgtcc
gtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctctt
gcccggcgtcaatacgggataataccgcgccacagagcagaactttaaaagtgctcatcattgggaaacgttcttcggg
gcgaaaactctcaagaatcttaccgctgttgaggtccagttcgatgtaacccacacgtgcacccaactgatcttcggca
tcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacac
ggaaatgttgaatactcatattcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggata
gatatttgaatgtatttaggctagctccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccg
aaaagattacttcgcgtttgccacctgacgtctaagaaaaggaatattcagcaatttgcccgtgccgaagaaaggccca
cccgtgaaggtgagccagtgagttgattgctacgtaattagttagttagcccttagtgactcgaattcgcggccgcttc
tagagggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatat
gtatacccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtc
tgtttgtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgct
gtcagataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatg
gccagtgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccatta
acctgatgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaat
ctgctacttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccg
aaggtaactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaaga
actctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtct
taccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagccc
agcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaaggga
gaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctg
gtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagc
ctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat