BioBrick base vector (for constructing pSB**5 vectors)
This is a composite part generated from several basic parts obtained from a variety of sources.
This is a vector scaffold for constructing new BioBricks vectors. Information on the design is available at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design
Documented at http://openwetware.org/wiki/Synthetic_Biology:Vectors/pSB%2A%2A5_design Due to technical difficulties during synthesis, rather than including BBa_P1011 as designed, BBa_P1016 was synthesized as a component of this part. Hence, BBa_I51000 documents the original design and BBa_I51001 is the vector scaffold as synthesized. BBa_I52002 is the base vector as corrected from the synthesized construct.
Topoisomerase I mediated cloning site (reverse)
Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning. This site belongs 3' of the cloning site. It is the reverse complement of BBa_B0043.
This part is the standard BioBricks suffix.
Minimal pUC19-derived high copy replication origin
High copy origin of replication derived from pSB1A3. Designed to omit antibiotic resistance markers and primer binding sites. Lacks mutations that render BBa_I50020 nonfunctional as a replication origin.
We had to leave several restriction sites in because point mutations that eliminated these sites were found to render the origin nonfunctional.
This part is derived from BBa_P1010 but it lacks ccdA-.
This part is a differs from BBa_P1011 in that the mutations introduced in BBa_P1011 to remove restriction sites are not present in this part. It does have the "normal" ccd promoter and ccdB coding region. It also has a double TAA stop codon as per BioBrick conventions.
ccdA- was eliminated since it is an inactive form of ccdA and should not be required for the toxic phenotype of ccdB.
This part contains a stop codon in all 6 reading frames. Useful for ensuring that translation does not proceed through this part.
Topoisomerase I mediated cloning site (forward)
Insertion of this site will enable generation of a TOPO vector. The vector will have a T overhang and permit binding of topoisomerase I for direct cloning of PCR products. See http://openwetware.org/wiki/Topoisomerase_I_mediated_TA_cloning for a complete description of Topoisomerase I mediated cloning.
NheI restriction enzyme site (XbaI, SpeI compatible overhang)
NheI recognition site. Digestion with NheI generates a compatible cohesive end to XbaI and SpeI.
Terminator to be placed downstream of multiple cloning site in new pSB series plasmids. Similar to BBa_B0051.
identical to E. coli MG1655 between tonB and yciA
Terminator to be placed upstream of multiple cloning site in new pSB series plasmids.
Reverse of B0052, E.coli transcriptional terminator from the ribosomal RNA rrnC operon.
This part is the standard BioBricks prefix.
Forward primer for sequencing/amplifying BioBrick parts (VF2)
Originally named VF2, primer binds upstream of the standard BBa_MCS on biobrick vectors.
Reverse BioBrick primer annealing site (VR binding site)
This is the VR sequence to be used in construction of plasmids which include the VR primer site. It is the reverse complement of BBa_G00101.
ampicillin resistance cassette, reverse orientation
This contains TetR, a terminator and then AmpR and a terminator
Conversion of the iGEM parts registry to SBOL2.1