LOCUS       BBa_I5120                937 bp    DNA     linear       20-Dec-2024
DEFINITION  434 cI regulatable expression of YFP
ACCESSION   BBa_I5120
VERSION     BBa_I5120.1  
FEATURES             Location/Qualifiers
     misc_feature    1..43
                     /label=OR2
     misc_feature    8..21
                     /label=OR2
     misc_feature    30..43
                     /label=OR1
     promoter        1..6
                     /label=-35
     promoter        24..29
                     /label=-10
     promoter        2..7
                     /label=-35
     misc_feature    35..37
                     /label=start
     misc_feature    1..46
                     /label=BBa_R0052
     promoter        1..46
                     /label=BBa_R0052
     misc_feature    59..62
                     /label=conserved
     RBS             55..66
                     /label=BBa_B0034
     CDS             73..834
                     /label=YFP (LVA)
     misc_feature    829..834
                     /label=2
     misc_feature    790..828
                     /label=SsrA
     misc_feature    314..314
                     /label=A (Q->L)
     misc_feature    73..834
                     /label=BBa_E0032
     misc_feature    73..834
                     /label=BBa_E0032
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     stem_loop       904..926
                     /label=stem_loop
     misc_feature    892..937
                     /label=BBa_B0011
     terminator      892..937
                     /label=BBa_B0011
ORIGIN
        1 ttgacaaaca agatacattg tatgaaaata caagaaagtt tgttgatact agagaaagag
       61 gagaaatact agatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      121 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      181 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      241 ccctggccca ccctcgtgac caccttcggc tacggcctgc aatgcttcgc ccgctacccc
      301 gaccacatga agctgcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      361 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      421 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      481 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      541 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      601 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      661 cccgacaacc actacctgag ctaccagtcc gccctgagca aagaccccaa cgagaagcgc
      721 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
      781 ctgtacaaga ggcctgctgc aaacgacgaa aactacgctt tagtagctta ataatactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gagagaatat
      901 aaaaagccag attattaatc cggctttttt attattt
//