LOCUS       BBa_I52001              1090 bp    DNA     linear       07-May-2024
DEFINITION  ccdB and nonfunctional pUC19 derived high copy origin
ACCESSION   BBa_I52001
VERSION     BBa_I52001.1  
FEATURES             Location/Qualifiers
     promoter        41..46
                     /label=-35
     promoter        64..69
                     /label=-10
     CDS             108..416
                     /label=ccdB
     misc_feature    1..416
                     /label=BBa_P1016
     misc_feature    432..1046
                     /label=rep (pMB1)
     misc_feature    1031..1031
                     /label=ORI
     misc_feature    443..1031
                     /label=origin of replication
     misc_feature    479..1031
                     /label=RNAII transcript
     promoter        620..625
                     /label=RNAI transcript promoter -35
     promoter        598..603
                     /label=RNAI transcript promoter -10
     misc_feature    482..589
                     /label=RNAI transcript
     promoter        464..469
                     /label=RNAII transcript promoter -10 sequence
     promoter        443..448
                     /label=RNAII transcript promoter -35 sequence
     misc_feature    677..677
                     /label=G to A, nonfunctional mutation
     misc_feature    681..681
                     /label=G to A, nonfunctional mutation
     misc_feature    690..690
                     /label=G to A, nonfunctional mutation
     misc_feature    719..719
                     /label=C to T, nonfunctional mutation
     misc_feature    883..883
                     /label=C to T, nonfunctional mutation
     misc_feature    417..1090
                     /label=BBa_I50020
ORIGIN
        1 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
       61 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg cagtttaagg
      121 tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag agtgatatta
      181 ttgacacgcc cgggcgacgg atggtgatcc ccctggccag tgcacgtctg ctgtcagata
      241 aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg cgcatgatga
      301 ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct gatctcagcc
      361 accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata taataacccg
      421 tagaaaagat caaaagatct tcttgagatc ctttttttct gcgcgtaatc tgctacttgc
      481 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      541 tttttccgaa ggtaactggc tttagcagag cgcagatacc aaatactgtc cttctagtgt
      601 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      661 taatcctgtt accagtagct actgccagta gcgataagtc gtgtcttacc gggttggatt
      721 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      781 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      841 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tctggtaagc ggcagggtcg
      901 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      961 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1021 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1081 ttgctcacat
//