##gff-version 3
##sequence-region BBa_I52001 1 1090
BBa_I52001	.	plasmid	417	1090	.	+	0	ID=BBa_I50020;Name=BBa_I50020
BBa_I52001	.	sequence_feature	443	1031	.	+	0	ID=annotation1793680;Name=origin of replication;Parent=BBa_I50020
BBa_I52001	.	sequence_feature	432	1046	.	+	0	ID=annotation1782180;Name=rep (pMB1);Parent=BBa_I50020
BBa_I52001	.	sequence_alteration	681	681	.	+	0	ID=annotation1910688;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
BBa_I52001	.	sequence_alteration	719	719	.	+	0	ID=annotation1910690;Name=C to T, nonfunctional mutation;Parent=BBa_I50020
BBa_I52001	.	sequence_alteration	883	883	.	+	0	ID=annotation1910691;Name=C to T, nonfunctional mutation;Parent=BBa_I50020
BBa_I52001	.	sequence_alteration	690	690	.	+	0	ID=annotation1910689;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
BBa_I52001	.	promoter	620	625	.	+	0	ID=annotation1793682;Name=RNAI transcript promoter -35;Parent=BBa_I50020
BBa_I52001	.	sequence_alteration	677	677	.	+	0	ID=annotation1910687;Name=G to A, nonfunctional mutation;Parent=BBa_I50020
BBa_I52001	.	promoter	464	469	.	+	0	ID=annotation1793685;Name=RNAII transcript promoter -10 sequence;Parent=BBa_I50020
BBa_I52001	.	promoter	598	603	.	+	0	ID=annotation1793683;Name=RNAI transcript promoter -10;Parent=BBa_I50020
BBa_I52001	.	sequence_feature	1031	1031	.	+	0	ID=annotation1782181;Name=ORI;Parent=BBa_I50020
BBa_I52001	.	promoter	443	448	.	+	0	ID=annotation1793686;Name=RNAII transcript promoter -35 sequence;Parent=BBa_I50020
BBa_I52001	.	sequence_feature	479	1031	.	+	0	ID=annotation1793681;Name=RNAII transcript;Parent=BBa_I50020
BBa_I52001	.	sequence_feature	482	589	.	+	0	ID=annotation1793684;Name=RNAI transcript;Parent=BBa_I50020
BBa_I52001	.	engineered_region	1	416	.	+	0	ID=BBa_P1016;Name=BBa_P1016
BBa_I52001	.	promoter	41	46	.	+	0	ID=annotation1910684;Name=-35;Parent=BBa_P1016
BBa_I52001	.	promoter	64	69	.	+	0	ID=annotation1910685;Name=-10;Parent=BBa_P1016
BBa_I52001	.	CDS	108	416	.	+	0	ID=annotation1910686;Name=ccdB;Parent=BBa_P1016
>BBa_I52001
ggcttactaaaagccagataacagtatgcgtatttgcgcgctgatttttgcggtataagaatatatactgatatgtata
cccgaagtatgtcaaaaagaggtatgctatgcagtttaaggtttacacctataaaagagagagccgttatcgtctgttt
gtggatgtacagagtgatattattgacacgcccgggcgacggatggtgatccccctggccagtgcacgtctgctgtcag
ataaagtctcccgtgaactttacccggtggtgcatatcggggatgaaagctggcgcatgatgaccaccgatatggccag
tgtgccggtctccgttatcggggaagaagtggctgatctcagccaccgcgaaaatgacatcaaaaacgccattaacctg
atgttctggggaatataataacccgtagaaaagatcaaaagatcttcttgagatcctttttttctgcgcgtaatctgct
acttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggt
aactggctttagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactct
gtagcaccgcctacatacctcgctctgctaatcctgttaccagtagctactgccagtagcgataagtcgtgtcttaccg
ggttggattcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagctt
ggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaag
gcggacaggtatctggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatc
tttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatg
gaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacat