LOCUS BBa_I5210 3174 bp DNA linear 20-Apr-2024 DEFINITION Olmecs: Repressilator version 2 (Lambda cI) ACCESSION BBa_I5210 VERSION BBa_I5210.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..766 /label=BBa_C0040 stem_loop 782..801 /label=T7 TE misc_feature 808..808 /label=stop polyA_site 802..815 /label=polya misc_feature 775..815 /label=BBa_B0012 terminator 775..815 /label=BBa_B0012 stem_loop 836..858 /label=stem_loop misc_feature 824..869 /label=BBa_B0011 terminator 824..869 /label=BBa_B0011 misc_feature 878..896 /label=TetR 1 misc_feature 878..931 /label=BBa_R0040 promoter 897..902 /label=-35 misc_feature 903..921 /label=TetR 2 promoter 920..925 /label=-10 promoter 878..931 /label=BBa_R0040 misc_feature 944..947 /label=conserved RBS 940..951 /label=BBa_B0034 CDS 961..1668 /label=cI lambda misc_feature 1669..1701 /label=LVA misc_feature 1708..1732 /label=Help:Barcodes CDS 958..1732 /label=BBa_C0051 stem_loop 1748..1767 /label=T7 TE misc_feature 1774..1774 /label=stop polyA_site 1768..1781 /label=polya misc_feature 1741..1781 /label=BBa_B0012 terminator 1741..1781 /label=BBa_B0012 stem_loop 1802..1824 /label=stem_loop misc_feature 1790..1835 /label=BBa_B0011 terminator 1790..1835 /label=BBa_B0011 promoter 1881..1886 /label=-10 promoter 1858..1863 /label=-35 misc_feature 1868..1884 /label=OR1 misc_feature 1844..1860 /label=OR2 misc_feature 1844..1892 /label=BBa_R0051 promoter 1844..1892 /label=BBa_R0051 misc_feature 1905..1908 /label=conserved RBS 1901..1912 /label=BBa_B0034 misc_feature 3008..3046 /label=LVA CDS 1919..3046 /label=lacI-LVA misc_feature 1919..3046 /label=BBa_C0012 misc_feature 3047..3071 /label=Help:Barcodes CDS 1919..3046 /label=BBa_C0012 stem_loop 3087..3106 /label=T7 TE misc_feature 3113..3113 /label=stop polyA_site 3107..3120 /label=polya misc_feature 3080..3120 /label=BBa_B0012 terminator 3080..3120 /label=BBa_B0012 stem_loop 3141..3163 /label=stem_loop misc_feature 3129..3174 /label=BBa_B0011 terminator 3129..3174 /label=BBa_B0011 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagtcacac 781 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc 841 agattattaa tccggctttt ttattatttt actagagtcc ctatcagtga tagagattga 901 catccctatc agtgatagag atactgagca ctactagaga aagaggagaa atactagatg 961 agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg ccttaaagca 1021 atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc agacaagatg 1081 gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt aaatgcttat 1141 aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag cccttcaatc 1201 gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact tagaagtgag 1261 tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga gcttagaacc 1321 tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag tgattctgca 1381 ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa gccaagcttt 1441 cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg tgatttctgc 1501 atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga tagcggtcag 1561 gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga gagttgttcc 1621 gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac 1681 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag 1741 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa 1801 aaagccagat tattaatccg gcttttttat tattttacta gagtaacacc gtgcgtgttg 1861 actattttac ctctggcggt gataatggtt gctactagag aaagaggaga aatactagat 1921 ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg tctcttatca 1981 gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc gggaaaaagt 2041 ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac aactggcggg 2101 caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg cgccgtcgca 2161 aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat 2221 ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg 2281 cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg ctgtggaagc 2341 tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac ccatcaacag 2401 tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg tcgcattggg 2461 tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc gtctgcgtct 2521 ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg 2581 cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg agggcatcgt 2641 tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc gcgccattac 2701 cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg ataccgaaga 2761 cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc tgctggggca 2821 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct 2881 gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc aaaccgcctc 2941 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 3001 cgggcaggct gcaaacgacg aaaactacgc tttagtagct taataactct gatagtgcta 3061 gtgtagatct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 3121 tactagagag agaatataaa aagccagatt attaatccgg cttttttatt attt //