LOCUS BBa_I5220 3090 bp DNA linear 26-Apr-2024 DEFINITION Olmecs: Repressilator version 3 (434 cI) ACCESSION BBa_I5220 VERSION BBa_I5220.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..766 /label=BBa_C0040 stem_loop 782..801 /label=T7 TE misc_feature 808..808 /label=stop polyA_site 802..815 /label=polya misc_feature 775..815 /label=BBa_B0012 terminator 775..815 /label=BBa_B0012 stem_loop 836..858 /label=stem_loop misc_feature 824..869 /label=BBa_B0011 terminator 824..869 /label=BBa_B0011 misc_feature 878..896 /label=TetR 1 misc_feature 878..931 /label=BBa_R0040 promoter 897..902 /label=-35 misc_feature 903..921 /label=TetR 2 promoter 920..925 /label=-10 promoter 878..931 /label=BBa_R0040 misc_feature 944..947 /label=conserved RBS 940..951 /label=BBa_B0034 CDS 958..1626 /label=cI 434 misc_feature 1588..1626 /label=LVA misc_feature 958..1626 /label=BBa_C0052 misc_feature 1627..1651 /label=Help:Barcodes CDS 958..1626 /label=BBa_C0052 stem_loop 1667..1686 /label=T7 TE misc_feature 1693..1693 /label=stop polyA_site 1687..1700 /label=polya misc_feature 1660..1700 /label=BBa_B0012 terminator 1660..1700 /label=BBa_B0012 stem_loop 1721..1743 /label=stem_loop misc_feature 1709..1754 /label=BBa_B0011 terminator 1709..1754 /label=BBa_B0011 misc_feature 1763..1805 /label=OR2 misc_feature 1770..1783 /label=OR2 misc_feature 1792..1805 /label=OR1 promoter 1763..1768 /label=-35 promoter 1786..1791 /label=-10 promoter 1764..1769 /label=-35 misc_feature 1797..1799 /label=start misc_feature 1763..1808 /label=BBa_R0052 promoter 1763..1808 /label=BBa_R0052 misc_feature 1821..1824 /label=conserved RBS 1817..1828 /label=BBa_B0034 misc_feature 2924..2962 /label=LVA CDS 1835..2962 /label=lacI-LVA misc_feature 1835..2962 /label=BBa_C0012 misc_feature 2963..2987 /label=Help:Barcodes CDS 1835..2962 /label=BBa_C0012 stem_loop 3003..3022 /label=T7 TE misc_feature 3029..3029 /label=stop polyA_site 3023..3036 /label=polya misc_feature 2996..3036 /label=BBa_B0012 terminator 2996..3036 /label=BBa_B0012 stem_loop 3057..3079 /label=stem_loop misc_feature 3045..3090 /label=BBa_B0011 terminator 3045..3090 /label=BBa_B0011 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagtcacac 781 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc 841 agattattaa tccggctttt ttattatttt actagagtcc ctatcagtga tagagattga 901 catccctatc agtgatagag atactgagca ctactagaga aagaggagaa atactagatg 961 agtatttctt ccagggtaaa aagcaaaaga atccagcttg gacttaacca ggctgaactt 1021 gctcaaaagg tggggactac ccagcagtct atagagcagc tcgaaaacgg taaaactaag 1081 cgaccacgct ttttaccaga acttgcgtca gctcttggcg taagtgttga ctggctgctc 1141 aatggcacct ctgattcgaa tgttagattt gttgggcacg ttgagcccaa agggaaatat 1201 ccattgatta gcatggttag agctggttcg tggtgtgaag cttgtgaacc ctacgatatc 1261 aaggacattg atgaatggta tgacagtgac gttaacttat taggcaatgg attctggctg 1321 aaggttgaag gtgattccat gacctcacct gtaggtcaaa gcatccctga aggtcatatg 1381 gtgttagtag atactggacg ggagccagtg aatggaagcc ttgttgtagc caaactgact 1441 gacgcgaacg aagcaacatt caagaaactg gtcatagatg gcggtcagaa gtacctgaaa 1501 ggcctgaatc cttcatggcc tatgactcct atcaacggaa actgcaagat tatcggtgtt 1561 gtcgtggaag cgagggtaaa attcgtagct gcaaacgacg aaaactacgc tttagtagct 1621 taataactct gatagtgcta gtgtagatct ctactagagt cacactggct caccttcggg 1681 tgggcctttc tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg 1741 cttttttatt attttactag agttgacaaa caagatacat tgtatgaaaa tacaagaaag 1801 tttgttgata ctagagaaag aggagaaata ctagatggtg aatgtgaaac cagtaacgtt 1861 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca 1921 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa 1981 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt 2041 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg 2101 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc 2161 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta 2221 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt 2281 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg 2341 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc 2401 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac 2461 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt 2521 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa 2581 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc 2641 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt 2701 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca 2761 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag 2821 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 2881 aatgcagctg gcacgacagg tttcccgact ggaaagcggg caggctgcaa acgacgaaaa 2941 ctacgcttta gtagcttaat aactctgata gtgctagtgt agatctctac tagagtcaca 3001 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagagagaa tataaaaagc 3061 cagattatta atccggcttt tttattattt //