LOCUS BBa_I5230 3116 bp DNA linear 25-Apr-2024 DEFINITION Olmecs: Repressilator version 4 (P22 c2) ACCESSION BBa_I5230 VERSION BBa_I5230.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 CDS 85..701 /label=tetR misc_feature 702..735 /label=SsrA misc_feature 742..766 /label=Help:Barcodes CDS 82..766 /label=BBa_C0040 stem_loop 782..801 /label=T7 TE misc_feature 808..808 /label=stop polyA_site 802..815 /label=polya misc_feature 775..815 /label=BBa_B0012 terminator 775..815 /label=BBa_B0012 stem_loop 836..858 /label=stem_loop misc_feature 824..869 /label=BBa_B0011 terminator 824..869 /label=BBa_B0011 misc_feature 878..896 /label=TetR 1 misc_feature 878..931 /label=BBa_R0040 promoter 897..902 /label=-35 misc_feature 903..921 /label=TetR 2 promoter 920..925 /label=-10 promoter 878..931 /label=BBa_R0040 misc_feature 944..947 /label=conserved RBS 940..951 /label=BBa_B0034 CDS 958..1605 /label=cII p22 misc_feature 1606..1638 /label=LVA misc_feature 1639..1644 /label=stop misc_feature 958..1644 /label=BBa_C0053 misc_feature 1645..1669 /label=Help:Barcodes CDS 958..1644 /label=BBa_C0053 stem_loop 1685..1704 /label=T7 TE misc_feature 1711..1711 /label=stop polyA_site 1705..1718 /label=polya misc_feature 1678..1718 /label=BBa_B0012 terminator 1678..1718 /label=BBa_B0012 stem_loop 1739..1761 /label=stem_loop misc_feature 1727..1772 /label=BBa_B0011 terminator 1727..1772 /label=BBa_B0011 misc_feature 1781..1783 /label=OR3 misc_feature 1791..1808 /label=OR2 misc_feature 1814..1831 /label=OR1 promoter 1798..1803 /label=-35 promoter 1788..1793 /label=-35 promoter 1810..1815 /label=-10 misc_feature 1781..1834 /label=BBa_R0053 promoter 1781..1834 /label=BBa_R0053 misc_feature 1847..1850 /label=conserved RBS 1843..1854 /label=BBa_B0034 misc_feature 2950..2988 /label=LVA CDS 1861..2988 /label=lacI-LVA misc_feature 1861..2988 /label=BBa_C0012 misc_feature 2989..3013 /label=Help:Barcodes CDS 1861..2988 /label=BBa_C0012 stem_loop 3029..3048 /label=T7 TE misc_feature 3055..3055 /label=stop polyA_site 3049..3062 /label=polya misc_feature 3022..3062 /label=BBa_B0012 terminator 3022..3062 /label=BBa_B0012 stem_loop 3083..3105 /label=stem_loop misc_feature 3071..3116 /label=BBa_B0011 terminator 3071..3116 /label=BBa_B0011 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca 121 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag 181 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac 241 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa 301 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat 361 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc 421 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca 481 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc 541 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct 601 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc 661 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata acactgatag tgctagtgta gatcactact agagtcacac 781 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc 841 agattattaa tccggctttt ttattatttt actagagtcc ctatcagtga tagagattga 901 catccctatc agtgatagag atactgagca ctactagaga aagaggagaa atactagatg 961 aatacacaat tgatgggtga gcgtattcgc gctcgaagaa aaaaactcaa gattagacaa 1021 gccgctcttg gtaagatggt gggagtgtct aatgttgcaa tatcgcaatg ggagcgctcg 1081 gagactgagc caaatgggga gaacctgttg gcactttcga aggctcttca gtgctcccct 1141 gactatttgc tgaaaggaga tttaagccag acaaacgttg cctatcatag taggcatgag 1201 ccaagaggat cataccctct tatcagttgg gtaagcgcag ggcaatggat ggaagctgta 1261 gaaccttatc acaagcgcgc gatagagaac tggcacgaca ccactgtaga ttgttcagaa 1321 gattcatttt ggcttgatgt ccaaggtgac tctatgacag caccggcagg gttaagcatt 1381 ccagaaggaa tgataattct ggttgatccc gaagtcgaac caagaaacgg caagctggtt 1441 gttgcaaaat tagaaggtga aaacgaggcc acattcaaaa aattagttat ggatgcaggc 1501 cgaaagtttt taaaaccatt aaacccacaa tatccgatga tagaaatcaa cggaaactgc 1561 aaaatcattg gcgtagttgt tgacgcaaaa ctcgcaaatc ttccagctgc aaacgacgaa 1621 aactacgctt tagtagctta ataacactga tagtgctagt gtagatcact actagagtca 1681 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagagag aatataaaaa 1741 gccagattat taatccggct tttttattat tttactagag aataaacttg actaaagatt 1801 cctttagtag ataatttaag tgttctttaa tttctactag agaaagagga gaaatactag 1861 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 1921 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 1981 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 2041 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 2101 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 2161 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 2221 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 2281 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 2341 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 2401 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 2461 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 2521 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 2581 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 2641 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 2701 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 2761 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 2821 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 2881 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 2941 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc 3001 tagtgtagat ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta 3061 tatactagag agagaatata aaaagccaga ttattaatcc ggctttttta ttattt //