LOCUS       BBa_I529201              285 bp    DNA     linear       26-Jun-2024
DEFINITION  HappyBirthdayDE Cassette
ACCESSION   BBa_I529201
VERSION     BBa_I529201.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             83..148
                     /label=BBa_I20529
     stem_loop       168..211
                     /label=stem_loop
     misc_feature    157..236
                     /label=BBa_B0010
     terminator      157..236
                     /label=BBa_B0010
     stem_loop       252..271
                     /label=T7 TE
     misc_feature    278..278
                     /label=stop
     polyA_site      272..285
                     /label=polya
     misc_feature    245..285
                     /label=BBa_B0012
     terminator      245..285
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag aggtggagag gtaccacgct cctccttacg atatcaggac
      121 ccaggatgct tacgataggg agtggtaata ctagagccag gcatcaaata aaacgaaagg
      181 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
      241 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//