##gff-version 3
##sequence-region BBa_I529201 1 285
BBa_I529201	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_I529201	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_I529201	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_I529201	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_I529201	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_I529201	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_I529201	.	terminator	245	285	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I529201	.	stem_loop	252	271	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I529201	.	stop_codon	278	278	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I529201	.	engineered_region	245	285	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I529201	.	polyA_site	272	285	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_I529201	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I529201	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I529201	.	terminator	157	236	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_I529201	.	engineered_region	157	236	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_I529201	.	stem_loop	168	211	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_I529201	.	CDS	83	148	.	+	0	ID=BBa_I20529;Name=BBa_I20529
>BBa_I529201
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gaggtggagaggtaccacgctcctccttacgatatcaggacccaggatgcttacgatagggagtggtaatactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttata