LOCUS       BBa_I5300                945 bp    DNA     linear       21-Dec-2024
DEFINITION  Olmecs: Reporter 1 of Synchro-Dualator
ACCESSION   BBa_I5300
VERSION     BBa_I5300.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..842
                     /label=CFP (LVA)
     misc_feature    799..836
                     /label=SsrA
     misc_feature    837..842
                     /label=2
     misc_feature    149..149
                     /label=A
     misc_feature    81..842
                     /label=BBa_E0022
     misc_feature    81..842
                     /label=BBa_E0022
     stem_loop       858..877
                     /label=T7 TE
     misc_feature    884..884
                     /label=stop
     polyA_site      878..891
                     /label=polya
     misc_feature    851..891
                     /label=BBa_B0012
     terminator      851..891
                     /label=BBa_B0012
     stem_loop       912..934
                     /label=stem_loop
     misc_feature    900..945
                     /label=BBa_B0011
     terminator      900..945
                     /label=BBa_B0011
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggtgagca agggcgagga gctgttcacc ggggtggtgc
      121 ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg tccggcgagg
      181 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      241 tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc
      301 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      361 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      421 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      481 acggcaacat cctggggcac aagctggagt acaactacat cagccacaac gtctatatca
      541 ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacatcgagg
      601 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      661 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      721 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      781 tggacgagct gtacaagagg cctgctgcaa acgacgaaaa ctacgcttta gtagcttaat
      841 aatactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga
      901 gagaatataa aaagccagat tattaatccg gcttttttat tattt
//