LOCUS BBa_I53325 2845 bp DNA linear 28-Nov-2024 DEFINITION p15A.Tet ACCESSION BBa_I53325 VERSION BBa_I53325.1 FEATURES Location/Qualifiers misc_feature 399..1223 /label=p15A origin of replication misc_feature 1223..1450 /label=repeat region misc_feature 1..1554 /label=BBa_I50032 promoter 1563..1651 /label=tetR promoter CDS 1652..2845 /label=tetA misc_feature 1563..2845 /label=BBa_P1005 ORIGIN 1 atggaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 61 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 121 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 181 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 241 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 301 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 361 cttaacgtga gttttcgttc cactgagcgt cagacccctt aataagatga tcttcttgag 421 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg 481 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag 541 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta 601 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg 661 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg 721 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg 781 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga 841 gcgcacgagg gagccgccag gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 901 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa 961 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga 1021 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg 1081 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg 1141 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata 1201 gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac 1261 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg 1321 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg 1381 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt 1441 caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata 1501 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgtttactag 1561 aggagattct catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca 1621 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat 1681 tctcggcacc gtcaccctgg acgctgtagg cataggcttg gttatgccgg tactgccggg 1741 cctcttgcgg gatatcgtcc attccgacag tattgccagt cactatggcg tgctgcttgc 1801 gctctatgcg ttgatgcaat ttctttgcgc acccgttctc ggagccctgt ccgaccgctt 1861 tggccgccgt ccagtcctgc tcgcttcgct ccttggagcc actatcgact acgcgatcat 1921 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggcgg gcatcacggg 1981 tgccacaggt gcggttgctg gtgcctatat cgccgacatc accgatgggg aagatcgggc 2041 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 2101 cgggggactg ttgggtgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa 2161 cggcctcaac ctcctcctgg gctgcttcct tatgcaggaa tcgcataagg gagagcgccg 2221 tccgatgccc ttgcgtgcct tcaatccagt cagctccttc cggtgggcgc ggggcatgac 2281 tatcgtcgcc gcacttatga ctgttttctt tatcatgcaa ctcgtaggac aggttccggc 2341 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 2401 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca cgggccccgc 2461 caccaaacgt ttcggcgaga agcaggccat tatcgcgggc atggcggccg acgcgctggg 2521 ctacgtcttg ctggcgttcg cgacgcgcgg ctggatggcc ttccccatta tgattcttct 2581 cgcttccggc ggcatcggta tgcccgcgtt gcaggccatg ctgtcccgcc aagtagatga 2641 cgaccatcag ggacagcttc aagggtcgct cgcggctctt accagcctca cttcgatcat 2701 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 2761 atggattgta ggtgccgccc tttaccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 2821 gagccgggcc acctcgacct aataa // LOCUS BBa_I53000 2845 bp DNA linear 28-Nov-2024 DEFINITION p15A.Tet ACCESSION BBa_I53000 VERSION BBa_I53000.1 FEATURES Location/Qualifiers misc_feature 399..1223 /label=p15A origin of replication misc_feature 1223..1450 /label=repeat region misc_feature 1..1554 /label=BBa_I50032 promoter 1563..1651 /label=tetR promoter CDS 1652..2845 /label=tetA misc_feature 1563..2845 /label=BBa_P1005 ORIGIN 1 atggaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 61 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 121 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 181 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 241 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 301 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 361 cttaacgtga gttttcgttc cactgagcgt cagacccctt aataagatga tcttcttgag 421 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg 481 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag 541 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta 601 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg 661 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg 721 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg 781 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga 841 gcgcacgagg gagccgccag gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 901 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa 961 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga 1021 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg 1081 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg 1141 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata 1201 gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac 1261 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg 1321 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg 1381 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt 1441 caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata 1501 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgtttactag 1561 aggagattct catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca 1621 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat 1681 tctcggcacc gtcaccctgg acgctgtagg cataggcttg gttatgccgg tactgccggg 1741 cctcttgcgg gatatcgtcc attccgacag tattgccagt cactatggcg tgctgcttgc 1801 gctctatgcg ttgatgcaat ttctttgcgc acccgttctc ggagccctgt ccgaccgctt 1861 tggccgccgt ccagtcctgc tcgcttcgct ccttggagcc actatcgact acgcgatcat 1921 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggcgg gcatcacggg 1981 tgccacaggt gcggttgctg gtgcctatat cgccgacatc accgatgggg aagatcgggc 2041 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 2101 cgggggactg ttgggtgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa 2161 cggcctcaac ctcctcctgg gctgcttcct tatgcaggaa tcgcataagg gagagcgccg 2221 tccgatgccc ttgcgtgcct tcaatccagt cagctccttc cggtgggcgc ggggcatgac 2281 tatcgtcgcc gcacttatga ctgttttctt tatcatgcaa ctcgtaggac aggttccggc 2341 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct 2401 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca cgggccccgc 2461 caccaaacgt ttcggcgaga agcaggccat tatcgcgggc atggcggccg acgcgctggg 2521 ctacgtcttg ctggcgttcg cgacgcgcgg ctggatggcc ttccccatta tgattcttct 2581 cgcttccggc ggcatcggta tgcccgcgtt gcaggccatg ctgtcccgcc aagtagatga 2641 cgaccatcag ggacagcttc aagggtcgct cgcggctctt accagcctca cttcgatcat 2701 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc 2761 atggattgta ggtgccgccc tttaccttgt ctgcctcccc gcgttgcgtc gcggtgcatg 2821 gagccgggcc acctcgacct aataa //