##gff-version 3
##sequence-region BBa_I5310 1 946
BBa_I5310	.	terminator	901	946	.	+	0	ID=BBa_B0011;Name=BBa_B0011
BBa_I5310	.	stem_loop	913	935	.	+	0	ID=annotation1683;Name=stem_loop;Parent=BBa_B0011
BBa_I5310	.	engineered_region	901	946	.	+	0	ID=annotation7019;Name=BBa_B0011;Parent=BBa_B0011
BBa_I5310	.	promoter	1	54	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_I5310	.	promoter	43	48	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_I5310	.	promoter	20	25	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_I5310	.	operator	26	42	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_I5310	.	engineered_region	1	54	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_I5310	.	operator	3	19	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_I5310	.	engineered_region	82	843	.	+	0	ID=BBa_E0032;Name=BBa_E0032
BBa_I5310	.	stop_codon	838	843	.	+	0	ID=annotation2159;Name=2;Parent=BBa_E0032
BBa_I5310	.	CDS	82	843	.	+	0	ID=annotation2156;Name=YFP (LVA);Parent=BBa_E0032
BBa_I5310	.	engineered_region	82	843	.	+	0	ID=annotation7041;Name=BBa_E0032;Parent=BBa_E0032
BBa_I5310	.	tag	799	837	.	+	0	ID=annotation2160;Name=SsrA;Parent=BBa_E0032
BBa_I5310	.	sequence_alteration	323	323	.	+	0	ID=annotation2161;Name=A (Q->L);Parent=BBa_E0032
BBa_I5310	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_I5310	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_I5310	.	terminator	852	892	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_I5310	.	engineered_region	852	892	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_I5310	.	stop_codon	885	885	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_I5310	.	stem_loop	859	878	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_I5310	.	polyA_site	879	892	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_I5310
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatact
agatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggcca
caagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggc
aagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcaatgcttcgcccgctaccccgaccaca
tgaagctgcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacgg
caactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttc
aaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagc
agaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactacca
gcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaa
gaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagc
tgtacaagaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt