LOCUS       BBa_I53322              2505 bp    DNA     linear       16-Dec-2024
DEFINITION  p15A.Amp
ACCESSION   BBa_I53322
VERSION     BBa_I53322.1  
FEATURES             Location/Qualifiers
     misc_feature    399..1223
                     /label=p15A origin of replication
     misc_feature    1223..1450
                     /label=repeat region
     misc_feature    1..1554
                     /label=BBa_I50032
     CDS             1642..2505
                     /label=ampicillin resistance
     promoter        1593..1598
                     /label=ampR -35
     promoter        1619..1624
                     /label=ampR -10
     RBS             1633..1637
                     /label=ampR RBS
     misc_feature    1563..2505
                     /label=BBa_P1002
ORIGIN
        1 atggaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
       61 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      121 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      181 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      241 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      301 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      361 cttaacgtga gttttcgttc cactgagcgt cagacccctt aataagatga tcttcttgag
      421 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg
      481 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag
      541 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta
      601 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg
      661 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
      721 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
      781 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
      841 gcgcacgagg gagccgccag gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      901 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
      961 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
     1021 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
     1081 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg
     1141 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata
     1201 gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac
     1261 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg
     1321 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg
     1381 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt
     1441 caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1501 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgtttactag
     1561 agctaaatac attcaaatat ctatccgctc atgagacaat aaccctgata aatgcttcaa
     1621 taatattgaa aaaggaagaa tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1681 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1741 gccgaagatc agttgggtgc acgtgtgggt tacatcgaac tggacctcaa cagcggtaag
     1801 attcttgaga gttttcgccc cgaagaacgt ttcccaatga tgagcacttt taaagttctg
     1861 ctctgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1921 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggac
     1981 ggcatgacag tacgcgaatt atgcagcgct gccataacca tgagtgataa cacggcggcc
     2041 aacttacttc tgacaacgat cggaggaccg aaggagctta ccgctttttt gcacaacatg
     2101 ggtgatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2161 gacgagcgtg acaccacgat gcctgtagct atggcaacaa cgttgcgcaa actcttaact
     2221 ggcgaacttc ttactctcgc ttcccggcaa caattaatag actggatgga ggcggataaa
     2281 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2341 ggagccggtg agcgtgggtc ccgcggtatt attgcagccc tggggccaga tggtaagccc
     2401 tcccgtatcg tagttatcta cacgacgggg agccaggcaa ctatggacga acgtaatcgc
     2461 cagatcgctg agataggtgc ctccctgatt aagcattggt aataa
//