LOCUS       BBa_I53325              2845 bp    DNA     linear       22-Dec-2024
DEFINITION  p15A.Tet
ACCESSION   BBa_I53325
VERSION     BBa_I53325.1  
FEATURES             Location/Qualifiers
     misc_feature    399..1223
                     /label=p15A origin of replication
     misc_feature    1223..1450
                     /label=repeat region
     misc_feature    1..1554
                     /label=BBa_I50032
     promoter        1563..1651
                     /label=tetR promoter
     CDS             1652..2845
                     /label=tetA
     misc_feature    1563..2845
                     /label=BBa_P1005
ORIGIN
        1 atggaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
       61 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      121 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      181 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      241 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      301 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      361 cttaacgtga gttttcgttc cactgagcgt cagacccctt aataagatga tcttcttgag
      421 atcgttttgg tctgcgcgta atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg
      481 gtttttcgaa ggttctctga gctaccaact ctttgaaccg aggtaactgg cttggaggag
      541 cgcagtcacc aaaacttgtc ctttcagttt agccttaacc ggcgcatgac ttcaagacta
      601 actcctctaa atcaattacc agtggctgct gccagtggtg cttttgcatg tctttccggg
      661 ttggactcaa gacgatagtt accggataag gcgcagcggt cggactgaac ggggggttcg
      721 tgcatacagt ccagcttgga gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg
      781 agacaaacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag gaacaggaga
      841 gcgcacgagg gagccgccag gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      901 caccactgat ttgagcgtca gatttcgtga tgcttgtcag gggggcggag cctatggaaa
      961 aacggctttg ccgcggccct ctcacttccc tgttaagtat cttcctggca tcttccagga
     1021 aatctccgcc ccgttcgtaa gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg
     1081 agtcagtgag cgaggaagcg gaatatatcc tgtatcacat attctgctga cgcaccggtg
     1141 cagccttttt tctcctgcca catgaagcac ttcactgaca ccctcatcag tgccaacata
     1201 gtaagccagt atacactccg ctagcgctga ggtctgcctc gtgaagaagg tgttgctgac
     1261 tcataccagg cctgaatcgc cccatcatcc agccagaaag tgagggagcc acggttgatg
     1321 agagctttgt tgtaggtgga ccagttggtg attttgaact tttgctttgc cacggaacgg
     1381 tctgcgttgt cgggaagatg cgtgatctga tccttcaact cagcaaaagt tcgatttatt
     1441 caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata
     1501 tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg tgtttactag
     1561 aggagattct catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca
     1621 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
     1681 tctcggcacc gtcaccctgg acgctgtagg cataggcttg gttatgccgg tactgccggg
     1741 cctcttgcgg gatatcgtcc attccgacag tattgccagt cactatggcg tgctgcttgc
     1801 gctctatgcg ttgatgcaat ttctttgcgc acccgttctc ggagccctgt ccgaccgctt
     1861 tggccgccgt ccagtcctgc tcgcttcgct ccttggagcc actatcgact acgcgatcat
     1921 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggcgg gcatcacggg
     1981 tgccacaggt gcggttgctg gtgcctatat cgccgacatc accgatgggg aagatcgggc
     2041 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     2101 cgggggactg ttgggtgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     2161 cggcctcaac ctcctcctgg gctgcttcct tatgcaggaa tcgcataagg gagagcgccg
     2221 tccgatgccc ttgcgtgcct tcaatccagt cagctccttc cggtgggcgc ggggcatgac
     2281 tatcgtcgcc gcacttatga ctgttttctt tatcatgcaa ctcgtaggac aggttccggc
     2341 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
     2401 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca cgggccccgc
     2461 caccaaacgt ttcggcgaga agcaggccat tatcgcgggc atggcggccg acgcgctggg
     2521 ctacgtcttg ctggcgttcg cgacgcgcgg ctggatggcc ttccccatta tgattcttct
     2581 cgcttccggc ggcatcggta tgcccgcgtt gcaggccatg ctgtcccgcc aagtagatga
     2641 cgaccatcag ggacagcttc aagggtcgct cgcggctctt accagcctca cttcgatcat
     2701 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
     2761 atggattgta ggtgccgccc tttaccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
     2821 gagccgggcc acctcgacct aataa
//